Author(s): Indira Prakoso, Alfero Putra Iryanto, Tiara Rahayu, Anzillina Rahma, Muhammad Nur Aziz Ar Rizqi, Viol Dhea Kharisma, Arif Nur Muhammad Ansori, Maksim Rebezov, Pavel Burkov, Marina Derkho, Belyakova Natalia, Rybakova Anna, Vikash Jakhmola, Rahadian Zainul

Email(s): rahadianzmsiphd@fmipa.unp.ac.id

DOI: 10.52711/0974-360X.2024.00003   

Address: Indira Prakoso1, Alfero Putra Iryanto2, Tiara Rahayu3, Anzillina Rahma4, Muhammad Nur Aziz Ar Rizqi5, Viol Dhea Kharisma6,7, Arif Nur Muhammad Ansori8,9, Maksim Rebezov10,11,12, Pavel Burkov13, Marina Derkho13, Belyakova Natalia14, Rybakova Anna15, Vikash Jakhmola9, Rahadian Zainul16,17*
1Department of Food Science and Biotechnology, Brawijaya University, Malang, Indonesia.
2Department of Biotechnology, Esa Unggul University, Jakarta Barat, Indonesia.
3Faculty of Military Mathematics and Natural Sciences, The Republic of Indonesia Defense University, Bogor, Indonesia.
4Department of Electrical Engineering, University of Indonesia, Depok, Indonesia.
5Department of Biology, Diponegoro University, Semarang, Indonesia.
6Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
7Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia.
8Faculty of Veterinary Medicine, Universitas Airlangg

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
Klebsiella pneumoniae is a gram-negative of bacteria that are known to cause a variety of nosocomial respiratory tract infections including pneumonia. K. pneumoniae is also included in the ESKAPE bacteria group which has high resistance to antibiotics. Therefore, alternative treatment for K. pneumoniae infection is needed, one of which is by developing a vaccine. The aim of this study was to design a vaccine against K. pneumoniae by targeting the outer membrane protein using immunoinformatics approaches. 1,708 protein of K. pneumoniae was then screened using signalP, pred-TMBB2, and Blastp to select outer membrane proteins. The selected protein, PA1_KLEPN and BAMA_KLEP7 were then predicted using T-and B-cell Epitope Prediction on IEDB to obtain epitope regions. Vaccine design of K. pneumoniae consists of 1 BCL epitope, 2 CTL epitopes, 1 HTL epitope, an adjuvant and PADRE sequences constructed with linkers using Benchling. This vaccine construction is predicted to be non-toxic/allergenic and have a strong binding affinity with human TLR-4 with the HADDOCK score of -93.2kcal/mol, RMSD 0.5 and Z-score -2.5. According to the computer-aided studies conducted for this study, the chosen epitopes may provide excellent vaccine candidates to stop K. pneumoniae infections in people. However, in order to further confirm the efficacy of this suggested vaccine candidate, in vitro and in vivo validation is required.


Cite this article:
Indira Prakoso, Alfero Putra Iryanto, Tiara Rahayu, Anzillina Rahma, Muhammad Nur Aziz Ar Rizqi, Viol Dhea Kharisma, Arif Nur Muhammad Ansori, Maksim Rebezov, Pavel Burkov, Marina Derkho, Belyakova Natalia, Rybakova Anna, Vikash Jakhmola, Rahadian Zainul. Multi-epitopes Vaccine Design against Klebsiella pneumoniae based on Outer Membrane Protein using Immunoinformatics Approaches. Research Journal of Pharmacy and Technology. 2024; 17(1):11-8. doi: 10.52711/0974-360X.2024.00003

Cite(Electronic):
Indira Prakoso, Alfero Putra Iryanto, Tiara Rahayu, Anzillina Rahma, Muhammad Nur Aziz Ar Rizqi, Viol Dhea Kharisma, Arif Nur Muhammad Ansori, Maksim Rebezov, Pavel Burkov, Marina Derkho, Belyakova Natalia, Rybakova Anna, Vikash Jakhmola, Rahadian Zainul. Multi-epitopes Vaccine Design against Klebsiella pneumoniae based on Outer Membrane Protein using Immunoinformatics Approaches. Research Journal of Pharmacy and Technology. 2024; 17(1):11-8. doi: 10.52711/0974-360X.2024.00003   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-3


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