MENU

Author(s): Suganthi M, Senthilkumar P, Abirami G, Thenmozhi M, Jayanthi M

Email(s): mpsenthilkumar@gmail.com

DOI: 10.5958/0974-360X.2020.00229.2   

Address: Suganthi M1, Senthilkumar P2, Abirami G1, Thenmozhi M1, Jayanthi M1
1Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram 600117, Tamil Nadu, India.
2Department of Genetic Engineering, SRM University, Kattankulathur 603203, Chennai, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 3,     Year - 2020


ABSTRACT:
Endophytes are endosymbiont, often a bacterium or fungus that lives within a plant for atleast part of its life cycle without causing apparent disease. Apart from the role of endophytes in host growth, nutrient acquisition, which decrease biotic stresses by enhancing plant resistance to insects, pathogens and herbivores. Replacement of chemical pesticides by alternate control measures is expected to make a significant contribution for the protection of human health. Chitin and chitinolytic enzyme are gaining importance for their biotechnological application, especially the chitinases exploited in agriculture fields to control pests/pathogens. In this view, present study initiated to isolate and screen the chitinolytic endophytic bacteria from Datura metel L. The bacterial isolates obtained will be very useful for the production of chitinase which can be employed for the biocontrol of harmful insects and pathogens. This study presents a first time report of chitinase producing endophytic bacteria from Datura metel L.


Cite this article:
Suganthi M, Senthilkumar P, Abirami G, Thenmozhi M, Jayanthi M. Isolation and Screening of chitinase producing endophytic bacteria from Datura metal L. Research J. Pharm. and Tech 2020; 13(3):1243- 1247. doi: 10.5958/0974-360X.2020.00229.2

Cite(Electronic):
Suganthi M, Senthilkumar P, Abirami G, Thenmozhi M, Jayanthi M. Isolation and Screening of chitinase producing endophytic bacteria from Datura metal L. Research J. Pharm. and Tech 2020; 13(3):1243- 1247. doi: 10.5958/0974-360X.2020.00229.2   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-3-34


REFERENCES:

1.     Ryan, R. P., Germaine, K., Ashley Franks, A., Ryan, D. J., Dowling, D. N. Bacterial endophytes: recent developments and applications. FEMS Microbiol Lett, 278; 2008:1-9.

2.     Bhattachrya, D., Nagpure, A., Gupta, R. K. Bacterial Chitinases: Properties and Potential. Crit Rev Biotechnol, 27(1); 2007: 21-8.

3.     Park, J. K., Kim, W. J., Park, Y. I. Purification and characterization of an exo-type β-N-acetylglucosaminidase from Pseudomonas fluorescens JK-0412. J Appl Microbiol, 110(1); 1996; 277-86.

4.     Mendonsa, E. S., Vartak, P. H., Rao, J. U., Deshpande, M.V. An enzyme from Myrothecium verrucaria that degrades insect cuticles for biocontrol of Aedes aegypti mosquito. Biotechnol Lett, 18(4); 1996: 373-6.

5.     Shanmugaiah, V., Mathivanan, N. Optimization of cultural conditions for productions of chitinase by Bacillius laterosporous isolated from rice rhizosphere soil. Afr J Biotechnol, 7; 2008: 2562-8.

6.     Karthika, S., Chellaram, C. Isolation, screening, and characterization of chitinase producing bacteria from marine wastes. Int J Pharm Pharm Sci, 8; 2016:ISSN- 0975-1491.

7.     Cook, R. J. Assuring the safe use of microbial biocontrol agents: a need for policy based on real rather than perceived risks. Can J Plant Pathol, 18; 1996: 439-45.

8.     Roberts, W. K., Selitrennikoff, C. P. Plant and bacterial chitinases differ in antifungal activity. J Gen Microbiol 134; 1988: 169-76.

9.     Anjum, N., Ramesh, C. Endophytic bacteria: optimizaton of isolation procedure from various medicinal plants and their preliminary characterization. Asian J Pharm Clin Res, 8; 2015: 233-8.

10. Moricca, S., Ragazzi, A., Mitchelson, K. R., Assante, G. Antagonism of the two-needle pine stem rust fungi Cronartium flaccidum and Peridermium pini by Cladosporium tenuissimum in vitro and in planta. Phytopathol, 91; 2001: 457-68.

11. Suganthi, M., Arvinth, S., Raj Kumar, R., Chandrashekara, K. N. Detection of chitinase activity and its characterization from Pseudomonas fluorescens of tea rhizosphere. J Plant Crops, 43; 2015: 236-9.

12. Kloepper, J. W., Rodriguez-Kabana, R., Zehnder, G. W., Murphy, J., Sikora, E., Fernandez, C. Plant root-bacterial interactions in biological control of soil borne diseases and potential extension to systemic and foliar diseases. Aust J Plant Pathol, 28; 1999: 27-33.

13. Gray, E. J., Smith. D. L. Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signalling processes. Soil Biol Biochem, 37; 2005: 395-412.

14. Compant, S., Duffy, B., Nowak, J., Barka, E. A. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl Environ Microbiol, 71; (2005a): 4951-9.

15. Kerry, B. R. Rhizosphere interactions and the exploitation of microbial agents for the biological control of plant-parasitic nematodes. Ann Rev Phytopath, 38; 2000: 423-41.

16. Shternshis, M. V., Ovchinnikova, L. A., Duzhak, A. B., Tomilova, O. G. The efficiency of the viral and bacterial entomopathogens formulated with chitinase for biocontrol of lepidopteran cabbage pests. Arch Phytopathol Plant Prot, 35; 2002:161-9.

17. Bisht, A., Thapliyal, M., Singh, A. Screening and isolation of antibacterial proteins/peptides from seeds of millets. Int J Curr Pharm Res, 8: 2016; 96-9.

18. Broadway, R. M., Gongora, C., Kain, W. C., Sanderson, J. P., Monroy, J. A., Bennett, K. C., Warner, J. P., Hoffmann, M. P. Novel chitinolytic enzymes with biological activity against herbivorous insects. J Chem Ecol, 24; 1998: 985-98.

19. Downing, K. J., Graeme, L., Jennifer, A., Thomson. Biocontrol of the Sugarcane Borer Eldana saccharina by Expression of the Bacillus thuringiensis cry1Ac7 and Serratia marcescens chiA Genes in Sugarcane-Associated Bacteria. Appl Environ Microbiol,  2000: 2804-10.

20. Kramer, K. J., Muthukrishnan, S. Insect chitinases: molecular biology and potential use as biopesticides. Insect Biochem Mol Biol, 27;1997: 887-900.

21. Ramesh R, Thushara Mathew, Nivedita Singh. Role of Chitinolytic enzymes and volatile compounds produced by endophytic bacteria in the inhibition of mango (Mangifera indica L.) root rot pathogens. J Biol Control, 23; 2009: 433-41.

22. Kramer, K. J., Koga, D. Insect chitin: physical state, synthesis, degradation and metabolic regulation. Insect Biochem, 16; 1986: 851-77.

23. Saima, M. K., Roohi, I. Z. A. Isolation of novel chitinolytic bacteria and production optimization of extracellular chitinase. J Genetic Eng Biotechnol, 11; 2013: 39-46.

24. Ramadhan, F., Mukarramah, L., Oktavia, F. R. H., Yulian, R., Annisyah, N. H., Asyiah, I. N. Flavonoids from endophytic bacteria of cosmos caudatus kunth. leaf as anticancer and antimicrobial. Asian J Pharm Clin Res, 11; 2018: 200-4.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available