Author(s):
Yulistia Budianti Soemarie, Hasniah, Aris Fadillah, Muliyani, Nily Su’aida, Nadia Rahmi, Achmad Jaelani
Email(s):
yulistiab@gmail.com
DOI:
10.52711/0974-360X.2026.00606 
Address:
Yulistia Budianti Soemarie1*, Hasniah1, Aris Fadillah1, Muliyani1, Nily Su’aida1, Nadia Rahmi1, Achmad Jaelani2
1Faculty of Pharmacy, Islamic University of Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin, South Kalimantan, Indonesia.
2Faculty of Agriculture, Islamic University of Kalimantan Muhammad Arsyad Al Banjari, Banjarmasin, South Kalimantan, Indonesia.
*Corresponding Author
Published In:
Volume - 19,
Issue - 9,
Year - 2026
ABSTRACT:
Fermentation is one of the food processing methods involving microbial activity to enhance the quality and shelf `life of food products. Tamba Udang Vaname is a traditional fermented food from North Kalimantan made from shrimp (Litopenaeus vannamei), rice and salt, with a fermentation process lasting 14 days. This study was conducted to identify the dominant bacteria involved in the fermentation of Tamba Udang Vaname from North Kalimantan, Indonesia, using molecular-based identification methods. The shrimp were collected in North Kalimantan and then fermented using a traditional local method. After 14 days, bacterial isolation, characterization, and identification using PCR–16S rRNA were performed. The result identified four bacterial isolates, all Gram-positive with coccus morphology (two isolates) and bacillus morphology (two isolates). Sequencing analysis identified isolate E1 and E2 as Staphylococcus epidermidis (99.93% similarity; GenBank: NR_113957.1) and isolate B1 and B2 as Bacillus cereus (99.05% similarity; GenBank: NR_074540.1). In conclusion, Tamba Udang Vaname is dominated by Gram-positive halotolerant bacteria, namely Staphylococcus epidermidis and Bacillus cereus.
Cite this article:
Yulistia Budianti Soemarie, Hasniah, Aris Fadillah, Muliyani, Nily Su’aida, Nadia Rahmi, Achmad Jaelani. Molecular Identification of Dominant Bacteria in Tamba Udang Vaname (Litopenaeus vannamei) Fermentation from North Kalimantan, Indonesia. Research Journal Pharmacy and Technology. 2026;19(9):4341-6. doi: 10.52711/0974-360X.2026.00606
Cite(Electronic):
Yulistia Budianti Soemarie, Hasniah, Aris Fadillah, Muliyani, Nily Su’aida, Nadia Rahmi, Achmad Jaelani. Molecular Identification of Dominant Bacteria in Tamba Udang Vaname (Litopenaeus vannamei) Fermentation from North Kalimantan, Indonesia. Research Journal Pharmacy and Technology. 2026;19(9):4341-6. doi: 10.52711/0974-360X.2026.00606 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-9-52
REFERENCES:
1. Wati LA. Analyzing the development of Indonesia shrimp industry. IOP Conf Ser Earth Environ Sci. 2018; 137(1). doi:10.1088/1755-1315/137/1/012101
2. Dayal JS, Ponniah AG, Khan HI, Babu EPM, Ambasankar K, Vasagam KPK. Shrimps - a nutritional perspective. Curr Sci. 2013; 104(11): 1487-1491.
3. AlFaris NA, Alshammari GM, AlTamimi JZ, et al. Evaluating the effects of different processing methods on the nutritional composition of shrimp and the antioxidant activity of shrimp powder. Saudi J Biol Sci. 2022; 29(1): 640-649. doi:10.1016/j.sjbs.2021.09.029
4. Putra DF, Abbas MA, Siregar TN, Wowor D. First record on the freshwater shrimp and its ecology in Weh Island, Indonesia. Biodiversitas. 2025; 26(4): 1991-2001. doi:10.13057/biodiv/d260448
5. Farajallah A, Perwitasari-Farajallah D, Widayati K, Rachman D, Rismawati R, Purnamasari L. Notes on freshwater shrimp (Crustacea, Decapoda, Caridea) from Belitung, Indonesia. ARPHA Prepr. 2024; e120274: 1-23. https://preprints.arphahub.com/article/120274/download/pdf/%0Ahttps://doi.org/10.3897/arphapreprints.e120274%0Ahttps://preprints.arphahub.com/article/120274/
6. Kitessa DA. Review on effect of fermentation on physicochemical properties, anti-nutritional factors and sensory properties of cereal-based fermented foods and beverages. Ann Microbiol. 2024; 74(1). doi:10.1186/s13213-024-01763-w
7. Sharma R, Garg P, Kumar P, Bhatia SK, Kulshrestha S. Microbial fermentation and its role in quality improvement of fermented foods. Fermentation. 2020; 6(4): 1-20. doi:10.3390/fermentation6040106
8. Sawant SS, Park HY, Sim EY, Kim HS, Choi HS. Microbial Fermentation in Food: Impact on Functional Properties and Nutritional Enhancement—A Review of Recent Developments. Fermentation. 2025; 11(1). doi:10.3390/fermentation11010015
9. Gill A. The Importance Of Bacterial Culture To Food Microbiology In The Age Of Genomics. Front Microbiol. 2017; 8: 777.
10. Jalili M, Nazari M, Magkos F. Fermented Foods in the Management of Obesity: Mechanisms of Action and Future Challenges. Int J Mol Sci. 2023; 24(3). doi:10.3390/ijms24032665
11. Siddiqui SA, Erol Z, Rugji J, et al. An overview of fermentation in the food industry - looking back from a new perspective. Bioresour Bioprocess. 2023; 10(1). doi:10.1186/s40643-023-00702-y
12. Nuraida L. A Review: Health Promoting Lactic Acid Bacteria In Traditional Indonesian Fermented Foods. Food Sci Hum Wellness. 2015; 4(2): 47-55.
13. Adesemoye ET, Sanni AI, Spano G, Capozzi V, Fragasso M. Lactic Acid Bacteria Diversity in Fermented Foods as Potential Bio-Resources Contributing to Alleviate Malnutrition in Developing Countries: Nigeria as a Case Study. Fermentation. 2025; 11(2): 1-25. doi:10.3390/fermentation11020103
14. Soemarie Y, Pratama R, Milanda T, Barliana M. Characterization of Lactococcus garvieae Isolated from Wadi Papuyu (Anabas testudineus Bloch) Fermentation of Indonesian Origin as a Probiotic Candidate. Sci Pharm. 2025; 4(2): 89-95. doi:10.58920/sciphar0402317
15. Soemarie YB, Milanda T, Barliana MI. Isolation, Characterization, and Identification Candidate of Probiotic Bacteria Isolated from Wadi Papuyu (Anabas testudineus Bloch.) a Fermented Fish Product from Central Kalimantan, Indonesia. Int J Food Sci. 2022;2022. doi:10.1155/2022/4241531
16. Junitasari A, Rosahdi TD, Lestari YS. Isolation and Identification of Lactic Acid Bacteria form Chinese Cabbage Waste by 16s rRNA Amplification using The Polymerase Chain Reaction (PCR) Method. J Kim Val. 2023; 9(1): 136-144.
17. Prastujati AU, Hilmi M, Khusna A, Arief II, Makmur S, Maulida Q. Isolation and Identification of Lactic Acid Bacteria of Bekamal (Banyuwangi Traditional Fermented Meat). IOP Conf Ser Earth Environ Sci. 2022; 1020(1). doi:10.1088/1755-1315/1020/1/012026
18. Kyule DN, Maingi JM, Njeru EM, Nyamache AK. Molecular Characterization and Diversity of Bacteria Isolated from Fish and Fish Products Retailed in Kenyan Markets. Int J Food Sci. 2022; 2022. doi:10.1155/2022/2379323
19. Abdullah RH, Hassoni AA. A Novel Method of Extraction and Purification of Bacterial DNA for PCR Detection of MecA, 16SrRNA, VanB. Eur J Med Genet Clin Biol. 2024; 1(4): 43-51. doi:10.61796/jmgcb.v1i4.403
20. Hong Y, Guo M, Wang J. ENJ algorithm can construct triple phylogenetic trees. Mol Ther Nucleic Acids. 2021; 23(March): 286-293. doi:10.1016/j.omtn.2020.11.004
21. Tamang JP, Watanabe K, Holzapfel WH. Review: Diversity of microorganisms in global fermented foods and beverages. Front Microbiol. 2016;7(MAR). doi:10.3389/fmicb.2016.00377
22. Khairina R, Nooryantini N, Ristiarini S, Nata IF. Combination of Salt and Rice Ratio on The Nutritional and Microbiological Qualities of Ronto, a Shrimp Fermented Product from Kalimantan, Indonesia. Int J Adv Trop Food. 2021; 3(1): 23-34. doi:10.26877/ijatf.v3i1.9305
23. Sionek B, Szydłowska A, Küçükgöz K, Kołożyn-Krajewska D. Traditional and New Microorganisms in Lactic Acid Fermentation of Food. Fermentation. 2023; 9(12): 1-21. doi:10.3390/fermentation9121019
24. Anumudu CK, Miri T, Onyeaka H. Multifunctional Applications of Lactic Acid Bacteria: Enhancing Safety, Quality, and Nutritional Value in Foods and Fermented Beverages. Foods. 2024; 13(23). doi:10.3390/foods13233714
25. Shao X, Wang H, Song X, Xu N, Sun J, Xu X. Effects of different mixed starter cultures on microbial communities, taste and aroma compounds of traditional Chinese fermented sausages. Food Chem X. 2024; 21(December 2023). doi:10.1016/j.fochx.2024.101225
26. Brescó MS, Harris LG, Thompson K, et al. Pathogenic mechanisms and host interactions in Staphylococcus epidermidis device-related infection. Front Microbiol. 2017; 8(AUG). doi:10.3389/fmicb.2017.01401
27. Severn MM, Horswill AR. Staphylococcus epidermidis and its dual lifestyle in skin health and infection. Nat Rev Microbiol. 2023; 21(2): 97-111. doi:10.1038/s41579-022-00780-3
28. Ortega-Peña S, Rodríguez-Martínez S, Cancino-Diaz ME, Cancino-Diaz JC. Staphylococcus epidermidis Controls Opportunistic Pathogens in the Nose, Could It Help to Regulate SARS-CoV-2 (COVID-19) Infection? Life. 2022; 12(3). doi:10.3390/life12030341
29. Byczkowska-Rostkowska Z, Gajewska J, Chajęcka-Wierzchowska W. Whole genome analysis and antimicrobial resistance assessment of Staphylococcus epidermidis isolated from food sources. Sci Total Environ. 2025; 993(June). doi:10.1016/j.scitotenv.2025.179999
30. Yang X, Wang Z, Zhang C, et al. Assessment of the production of Bacillus cereus protease and its effect on the quality of ultra-high temperature-sterilized whole milk. J Dairy Sci. 2021; 104(6): 6577-6587. doi:10.3168/jds.2020-19818
31. Dietrich R, Jessberger N, Ehling-Schulz M, Märtlbauer E, Granum PE. The Food Poisoning Toxins of Bacillus cereus. Toxins (Basel). 2021; 13(2). doi:10.3390/TOXINS13020098
32. Koo OK, Lee SJ, Chung KR, Jang DJ, Yang HJ, Kwon DY. Korean traditional fermented fish products: jeotgal. J Ethn Foods. 2016; 3(2): 107-116. doi:10.1016/j.jef.2016.06.004
33. Jung SJ, Chae SW, Shin DH. Fermented Foods of Korea and Their Functionalities. Fermentation. 2022; 8(11). doi:10.3390/fermentation8110645
34. Narzary Y, Das S, Goyal AK, Lam SS, Sarma H, Sharma D. Fermented fish products in South and Southeast Asian cuisine: indigenous technology processes, nutrient composition, and cultural significance. J Ethn Foods. 2021; 8(1). doi:10.1186/s42779-021-00109-0
35. Lim JY, Choi YJ, Yu H, et al. Investigation of Metabolite Differences in Salted Shrimp Varieties during Fermentation. ACS Omega. 2023; 8(50): 47735-47745. doi:10.1021/acsomega.3c06046
36. Komprda T, Cwiková O, Kumbár V, et al. Key Factors Influencing Bacillus cereus Contamination in Hot Ready-to-Eat Meal Delivery. Foods. 2025; 14(15): 2605. doi:10.3390/foods14152605
37. Vatansever S, Vegi A, Garden-Robinson J, Hall III CA. The Effect of Fermentation on the Physicochemical Characteristics of Dry-Salted Vegetables. J Food Res. 2017; 6(5): 32. doi:10.5539/jfr.v6n5p32