ABSTRACT:
There are three new, modest, true and exact methods validated and developed on the criteria of ICH regulations for evaluation of Evogliptin tartrate in tablet and its pure form. Method 1: AUC by UV spectrophotometry technique utilizing 262 to 266nm for measurement of analyte in tablet. Method 2: 1st derivative spectrophotometry utilizing 258nm for estimation of analyte in the tablet. The range and linearity were found to be 270 to 10µg/ml for both methods. The standard and sample solutions were prepared from methanol. Less than 2 was found less than 2 and recovery study of both Method. Method 3 consists RP-HPLC method using enable C18 column and containing Water and Methanol mobile phase in the ratio of 30 and 70. The 1ml/min of flow rate, with detection at 267nm, and temperature 250C. 2.806min was the retention time found. 10-80µg/ml was the linearity range which found. Y = 4006.6714 x + 713.6349 was the linear regression equation and Correlation coefficient (r2) = 0.9999. Less than 2 is the %RSD in the robustness, reclamation and precision investigations of the programmed method. Reported method was robust, accurate and precise in nature. Method-3 is more accurate and precise in comparison to method 1 and 2.