Author(s):
Varsha M. Gaikwad, Omprakash G. Bhusnure
Email(s):
varsha_gaikwad10@yahoo.com
DOI:
10.52711/0974-360X.2026.00586 
Address:
Varsha M. Gaikwad1*, Omprakash G. Bhusnure2
1Department of Pharmaceutics, Channabasweshwar Pharmacy College (Degree), Latur - 413512, Maharashtra, India.
2Department of Quality Assurance, Channabasweshwar Pharmacy College (Degree), Latur - 413512, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 9,
Year - 2026
ABSTRACT:
An initial nanofiber (NF) formulation was developed using individual drug loading with sulfasalazine (SSZ) and quercetin (QCT). Subsequently, a dual drug-loaded system (SSZ and QCT) was prepared using a composite polymer matrix of polyvinylpyrrolidone K-90 (PVP K-90) and ethyl cellulose (EC). The electrospun NF formulation was optimized using the desirability function in Design Expert software, yielding an encapsulation efficiency (EE) of 88.75% and a nanofiber diameter (ND) of 374 nm. The independent process parameters investigated included applied voltage, tip-to-collector distance, and solution flow rate. The polymer solution concentration for electrospinning was optimized to ensure extensive chain entanglement, enabling the formation of defect-free nanofibers. Fibers produced with PVP K-90 alone exhibited favorable viscosity characteristics and were smooth, uniform, cylindrical, and free from beads. Similarly, fibers produced with the composite polymer system (PVP K-90 and EC) were also smooth and non-beaded, as confirmed by scanning electron microscopy (SEM) images. The incorporation of EC into the polymer blend resulted in nanofibers with a reduced diameter, The hydrophobic nature of EC contributed to sustained release of the herbal component. The dual polymer approach enabled tailored release profiles for each drug, making the composite NF formulation suitable for meeting specific therapeutic regimens.
Cite this article:
Varsha M. Gaikwad, Omprakash G. Bhusnure. Preparation and Characterization of Dual Drug Loaded PVP-EC Electrospun Nanofiber for Rheumatoid Arthritis. Research Journal Pharmacy and Technology. 2026;19(9):4193-0. doi: 10.52711/0974-360X.2026.00586
Cite(Electronic):
Varsha M. Gaikwad, Omprakash G. Bhusnure. Preparation and Characterization of Dual Drug Loaded PVP-EC Electrospun Nanofiber for Rheumatoid Arthritis. Research Journal Pharmacy and Technology. 2026;19(9):4193-0. doi: 10.52711/0974-360X.2026.00586 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-9-32
REFERENCES:
1. Akbari, S. et. al. Preparation and characterization of diclofenac transdermal patch using ethyl cellulose and polyvinyl alcohol by electrospinning: Journal of Drug Delivery Science and Technology. 2024; 92(January). https://doi.org/10.1016/j.jddst.2024.105359
2. Aparecida et.al. Development , characterization and evaluation of the dissolution profile of sulfasalazine suspensions. 2015; 51.
3. Bhusnure, O. G., Gholve, S. B. et. al. Novel 5-flurouracil-Embedded non-woven PVA - PVP electrospun nanofibers with enhanced anti-cancer efficacy: Formulation, evaluation and in vitro anti-cancer activity. Journal of Drug Delivery Science and Technology, 2021; 64(June). https://doi.org/10.1016/j.jddst.2021.102654
4. Dimitroulas, T., Sandoo, A., et.al. Rheumatoid Arthritis. In The Heart in Rheumatic, Autoimmune and Inflammatory Diseases: Pathophysiology, Clinical Aspects and Therapeutic Approaches; 2017; 129–165. Elsevier Inc. https://doi.org/10.1016/B978-0-12-803267-1.00006-5
5. Goel, R., Bhardwaj, S., et.al.Pharmaceutical excipients. In Dosage Forms, Formulation Developments and Regulations: Recent and Future Trends in Pharmaceutics. 2023; 1: 311–348 https://doi.org/10.1016/B978-0-323-91817-6.00003-6
6. Gokhale, J. P., Mahajan, H. S., et.al. Quercetin loaded nanoemulsion-based gel for rheumatoid arthritis: In vivo and in vitro studies. Biomedicine and Pharmacotherapy. 2019; 112 (January), 108622. https://doi.org/10.1016/j.biopha.2019.108622
7. Guan, F., Wang, Q., et.al. Anti-rheumatic effect of quercetin and recent developments in nano formulation. In RSC Advances. 2021; 11: 7280–7293 Royal Society of Chemistry. https://doi.org/10.1039/d0ra08817j
8. Haleagrahara, N., Hodgson, K., et.al. Flavonoid quercetin–methotrexate combination inhibits inflammatory mediators and matrix metalloproteinase expression, providing protection to joints in collagen-induced arthritis. Inflammopharmacology. 2018; 26(5): 1219–1232. https://doi.org/10.1007/s10787-018-0464-2
9. Janakiraman, K., Krishnaswami, V., et.al. Novel nano therapeutic materials for the effective treatment of rheumatoid arthritis-recent insights. Materials Today Communications. 2018; 17: 200–213. https://doi.org/10.1016/j.mtcomm.2018.09.011
10. Joshi, M., Pathak, K., et.al. Nanotechnology-based strategies for effective delivery of phytoconstituents for the management of rheumatoid arthritis. In Pharmacological Research - Modern Chinese Medicine. 2022; 2. Elsevier B.V. https://doi.org/10.1016/j.prmcm.2022.100061
11. Karthikeyan, K., Guhathakarta, S.,et.al. Electrospun zein/eudragit nanofibers based dual drug delivery system for the simultaneous delivery of aceclofenac and pantoprazole. International Journal of Pharmaceutics. 2012; 438(1–2): 117–122. https://doi.org/10.1016/j.ijpharm.2012.07.075
12. Köhler, B. M., Günther, J., et.al. Current therapeutic options in the treatment of rheumatoid arthritis. In Journal of Clinical Medicine. 2019; 8(7). MDPI. https://doi.org/10.3390/jcm8070938
13. Kour, G., Haq, S. A., et.al. Phytochemical add-on therapy to DMARDs therapy in rheumatoid arthritis: In vitro and in vivo bases, clinical evidence and future trends. In Pharmacological Research. 2021; 169 Academic Press. https://doi.org/10.1016/j.phrs.2021.105618
14. Kurakula, M., and Rao, et.al. Pharmaceutical assessment of polyvinylpyrrolidone (PVP): As excipient from conventional to controlled delivery systems with a spotlight on COVID-19 inhibition. Journal of Drug Delivery Science and Technology. 2020; 60; 9, 102046. https://doi.org/10.1016/j.jddst.2020.102046
15. Li, X., Li, Y., et.al. Fast Disintegrating Quercetin-Loaded Drug Delivery Systems Fabricated Using Coaxial Electrospinning. 2013; 21647–21659. https://doi.org/10.3390/ijms141121647
16. Monirul Islam, M., Hemmanahalli Ramesh, V., et.al. Optimization of process parameters for fabrication of electrospun nanofibers containing neomycin sulfate and Malva sylvestris extract for a better diabetic wound healing. Drug Delivery. 2022; 29(1): 3370–3383. https://doi.org/10.1080/10717544.2022.2144963
17. Naderi, A., Sheibani, A., et.al. Heliyon Theoretical and experimental studies on sulfasalazine interactions with poly (lactic acid): Impact of hydrogen bonding and charge transfer interactions on molecular structure, electronic and optical properties. Heliyon. 2024; 10(1): e23813. https://doi.org/10.1016/j.heliyon.2023.e23813
18. Nasra, S., Bhatia, D., et.al. Recent advances in nanoparticle-based drug delivery systems for rheumatoid arthritis treatment. In Nanoscale Advances Royal Society of Chemistry. 2022; 4; 17; 3479–3494 https://doi.org/10.1039/d2na00229a
19. Parham, S., Kharazi, A. Z., et.al. Electrospun Nano-fibers for biomedical and tissue engineering applications: A comprehensive review. In Materials. 2020; 13; 9. MDPI AG. https://doi.org/10.3390/ma13092153
20. Patil, R. B., Limbhore, et.al.Study of Solubility Enhancement of Quercetin by Inclusion Complexation with Betacyclodextrin. 2019; 11(80): 3102–3107.
21. Pelipenko, J., Kocbek, P., et.al. Critical attributes of nanofibers: Preparation, drug loading, and tissue regeneration. International Journal of Pharmaceutics. 2015; 484(1–2): 57–74. https://doi.org/10.1016/j.ijpharm.2015.02.043
22. Psimadas, D., Georgoulias, P., et.al. Molecular Nanomedicine Towards Cancer : Journal of Pharmaceutical Sciences. 2012; 101(7): 2271–2280. https://doi.org/10.1002/jps
23. Rains, C. P., Noble, S.,et.al. Erratum to Sulfasalazine: a review of its pharmacological properties and therapeutic efficacy in the treatment of rheumatoid arthritis. Drugs. 1995; 50(4): 625–625. https://doi.org/10.1007/BF03259121
24. Ravikumar, R., Ganesh, M., et.al. Tetrahydro curcumin loaded PCL-PEG electrospun transdermal nanofiber patch: Preparation, characterization, and in vitro diffusion evaluations. Journal of Drug Delivery Science and Technology. 2018; 44: 342–348. https://doi.org/10.1016/j.jddst.2018.01.016
25. Sa’adon, S., Ansari, et.al. Electrospun nanofiber and cryogel of polyvinyl alcohol transdermal patch containing diclofenac sodium: Preparation, characterization and in vitro release studies. Pharmaceutics. 2021; 13(11). https://doi.org/10.3390/pharmaceutics13111900
26. Sankpal, P. et.al. Evaluation of In-vitro Antidiabetic Activity of Quercetin Loaded Eudrgit S100 / GMO Nanoparticles. 2024; 13(2).
27. Shahriar, S. M. S., Mondal, et.al. Electrospinning nanofibers for therapeutics delivery. In Nanomaterials. 2019; 9; 4 MDPI AG. https://doi.org/10.3390/nano9040532
28. Sipos, E., Kósa, N.,et.al. Formulation and characterization of aceclofenac-loaded nanofiber based orally dissolving webs. Pharmaceutics. 2019; 11(8): 1–11. https://doi.org/10.3390/pharmaceutics11080417
29. The, I., Of, E., For, et.al. The Delivery Of Sulfasalazine : Improving The Effectiveness Of Treatment For Inflammatory Bowel Disease By Using Spray Drying Technique And Preformulation Study Of. World Journal of Pharmaceutical Developments. 2024; 13(16): 930–957. https://doi.org/10.20959/wjpr202416-33588
30. Wang, Q., Qin, X., et.al. Nanomedicines for the treatment of rheumatoid arthritis: State of art and potential therapeutic strategies. In Acta Pharmaceutica Sinica B: 2019; 11, 5; 1158–1174. Chinese Academy of Medical Sciences. https://doi.org/10.1016/j.apsb.2021.03.013
31. Zhao, J., Chen, X., et.al. Nanotechnology for diagnosis and therapy of rheumatoid arthritis: Evolution towards theranostic approaches. Chinese Chemical Letters. 2021; 32(1): 66–86. https://doi.org/10.1016/j.cclet.2020.11.048