Author(s):
K. Vara Prasada Rao, Sridevi Ranjitha Karanam, Y. Srinivasa Rao, P. Rohan Ratnakar, J. Kumari
Email(s):
ranjitha.karanam@gmail.com
DOI:
10.52711/0974-360X.2026.00578 
Address:
K. Vara Prasada Rao, Sridevi Ranjitha Karanam*, Y. Srinivasa Rao, P. Rohan Ratnakar, J. Kumari
Department of Pharmaceutical Analysis, Vignan Institute of Pharmaceutical Technology (Autonomous), Visakhapatnam, Andhra Pradesh - 530049, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 9,
Year - 2026
ABSTRACT:
A simple, precise, and eco-friendly UV spectroscopic technique was created and approved for the quantitative measurement of dutasteride in tablet and bulk medication forms of administration. The method employs ethanol as the solvent, with maximum absorbance observed at 213nm. With a correlation coefficient (r2) of 0.999, linearity was confirmed over the concentration range of 10–60µg/mL, suggesting a good linear connection between absorbance and concentration. Sandell's sensitivity was 0.0625µg/cm², and the molar absorptivity (e) was 6.63 × 10²L/mol/cm. The concentration was calculated using the regression equation y = 0.016x + 0.0084. High accuracy with an average recovery of 99.2% and precision with intraday and interday %RSD values of 1.62% and 1.35%, respectively, were proven by method validation carried out in compliance with ICH Q2(R1) requirements. With LOD and LOQ values of 0.37µg/mL and 1.11 µg/mL, respectively, the technique showed good sensitivity. Greenness assessment, conducted using the Analytical GREEnness (AGREE) metric and MoGAPI, indicated strong compliance with eco-friendly principles, with maximum scores for avoidance of derivatization, minimal waste generation, elimination of toxic reagents, and integration of processes to reduce energy use. Moderate scores were observed for low energy consumption and operator safety, while relatively lower scores were noted for in-situ applicability and the use of renewable-source reagents. The new approach is appropriate for continuous quality monitoring of dutasteride in the drug formulations since it is accurate, economical, and environmentally friendly.
Cite this article:
K. Vara Prasada Rao, Sridevi Ranjitha Karanam, Y. Srinivasa Rao, P. Rohan Ratnakar, J. Kumari. Development and Validation of a Green UV-spectroscopic Approach for Estimating Dutasteride in Tablet and Bulk forms of Administration with Greenness Evaluation by AGREE and MoGAPI. Research Journal Pharmacy and Technology. 2026;19(9):4126-2. doi: 10.52711/0974-360X.2026.00578
Cite(Electronic):
K. Vara Prasada Rao, Sridevi Ranjitha Karanam, Y. Srinivasa Rao, P. Rohan Ratnakar, J. Kumari. Development and Validation of a Green UV-spectroscopic Approach for Estimating Dutasteride in Tablet and Bulk forms of Administration with Greenness Evaluation by AGREE and MoGAPI. Research Journal Pharmacy and Technology. 2026;19(9):4126-2. doi: 10.52711/0974-360X.2026.00578 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-9-24
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