Author(s):
G. Sriram Prasath, U. S. Mahadeva Rao, S. Ragunandhakumar, Muhammed Anfas P M, Lee Wan Zhen, Norsuriani Samsudin
Email(s):
raousm@unisza.edu.my
DOI:
10.52711/0974-360X.2026.00567 
Address:
G. Sriram Prasath1, U. S. Mahadeva Rao2*, S. Ragunandhakumar3, Muhammed Anfas P M4, Lee Wan Zhen 5,6, Norsuriani Samsudin5
1Assistant Professor, Post graduate and Research Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam, Chennai-106, Tamilnadu India.
2Professor, School of Basic Medical Sciences, Faculty of Medicine, Kampus Perubatan, Universiti sultan Zainal Abdin 20400 Kuala Terengganu Malaysia.
3Associate Professor, Cancer and Stem Cell Research Lab, Department of Pharmacology, Saveetha Dental College, Chennai-600 077.
4Post Graduate Student, PG and Research Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam, Chennai-106 Tamilnadu India.
5Department of Wellness, Faculty of Hospitality, Tourism And Wellness (FHPK), Universiti Malaysia Kelantan, Kampus Kota, Kelantan, Malaysia.
6Exercise and Sports Science Programme, School of Health Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, M
Published In:
Volume - 19,
Issue - 9,
Year - 2026
ABSTRACT:
Cervical cancer is still a cause of death from cancer among women all over the world especially in countries that have low or medium income. This shows how important it is to find more effective ways to treat this disease. This study wanted to make silver nanoparticles using leaf extract from Ormocarpum cochinchinense in a way. The nanoparticles made were checked using UV– spectroscopy, Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). UV–Visible testing showed that the nanoparticles were made and had a peak at 396 nm. FTIR testing showed that the plant made groups that helped in making and keeping the nanoparticles stable. XRD showed that the silver had a crystal structure that's face-centred cubic. The study tested the ability of Ormocarpum cochinchinense-mediated silver nanoparticles (OC-AgNPs) to kill cancer cells. The OC-AgNPs killed cells in a way that depended on how much was used. The highest amount used 200 µg/mL caused the cell death, similar to cisplatin. The treatment made reactive oxygen species inside the cells and caused changes that are linked to cell death, such as cells shrinking, getting rounder not sticking to surfaces having their DNA pack tightly and their nuclei breaking apart. Staining with AO/EtBr showed cell death as the amount of OC-AgNPs increased. Overall, the results show that silver nanoparticles made with Ormocarpum cochinchinense have ability to fight cervical cancer cells. They might work by causing stress from oxygen species and, by making the cells die. More studies are needed to check if they're safe and how well they work in real life.
Cite this article:
G. Sriram Prasath, U. S. Mahadeva Rao, S. Ragunandhakumar, Muhammed Anfas P M, Lee Wan Zhen, Norsuriani Samsudin. Mechanistic Insights into ROS-Mediated Apoptosis and Migration Inhibition Induced by Green-Synthesized Ormocarpum cochinchinense Derived Silver Nanoparticles in Human Cervical Cancer Cells. Research Journal Pharmacy and Technology. 2026;19(9):4042-8. doi: 10.52711/0974-360X.2026.00567
Cite(Electronic):
G. Sriram Prasath, U. S. Mahadeva Rao, S. Ragunandhakumar, Muhammed Anfas P M, Lee Wan Zhen, Norsuriani Samsudin. Mechanistic Insights into ROS-Mediated Apoptosis and Migration Inhibition Induced by Green-Synthesized Ormocarpum cochinchinense Derived Silver Nanoparticles in Human Cervical Cancer Cells. Research Journal Pharmacy and Technology. 2026;19(9):4042-8. doi: 10.52711/0974-360X.2026.00567 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-9-13
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