Author(s):
Harshal Patil, Jyotsna Waghmare
Email(s):
harshalp54@gmail.com
DOI:
10.52711/0974-360X.2026.00528 
Address:
Harshal Patil, Jyotsna Waghmare
Department of Oils, Oleochemicals and Surfactant Technology, Institute of Chemical Technology (ICT), Nathalal Parikh Marg, Matunga (E), Mumbai - 400019, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 8,
Year - 2026
ABSTRACT:
This study explores the development of a natural makeup remover formulation using grapeseed oil microemulsions. The studies were conducted based on the synthesis of stable microemulsion systems using different non-ionic surfactants UNITOP HCO 40 and UNITOP Polysorbatee 80 in the absence of co-solvents. Optimized microemulsion compositions are determined by pseudo-ternary phase diagrams, and the derived formulations are evaluated for particle size, pH, conductivity, and stability. Two makeup remover formulations, MUR-1 and MUR-2, were prepared from the optimized microemulsions and tested for their cleaning ability against lipstick and liquid foundation. The particle sizes of the microemulsions were found to be in the range of 130-187nm, with good stability for 30 days. Both makeup remover formulations had excellent physical stability and cleaning efficiency, and the UNITOP HCO 40-based formulation was found to be more efficient in removing makeup. The development of a natural, stable, and effective makeup remover using grapeseed oil microemulsions is herein demonstrated.
Cite this article:
Harshal Patil, Jyotsna Waghmare. Formulation and Characterization of Grapeseed Oil-based Microemulsion Systems for Cosmetic Cleansing Applications. Research Journal of Pharmacy and Technology. 2026;19(8):3741-8-. doi: 10.52711/0974-360X.2026.00528
Cite(Electronic):
Harshal Patil, Jyotsna Waghmare. Formulation and Characterization of Grapeseed Oil-based Microemulsion Systems for Cosmetic Cleansing Applications. Research Journal of Pharmacy and Technology. 2026;19(8):3741-8-. doi: 10.52711/0974-360X.2026.00528 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-8-45
6. REFERENCE:
1. Shinagawa FB, Santana FCD, Araujo E, Purgatto E, Mancini-Filho J. Chemical composition of cold pressed Brazilian grape seed oil. Food Sci Technol. 2017; 38(1): 164-171. doi:10.1590/1678-457x.08317
2. P. Madawala SR, Kochhar SP, Dutta PC. Lipid components and oxidative status of selected specialty oils. Grasas Aceites. 2012; 63(2): 143-151. doi:10.3989/gya.083811
3. Nakamura Y, Tsuji S, Tonogai Y. Analysis of Proanthocyanidins in Grape Seed Extracts, Health Foods and Grape Seed Oils. J Health Sci. 2003; 49(1): 45-54. doi:10.1248/jhs.49.45
4. Passos CP, Silva RM, Da Silva FA, Coimbra MA, Silva CM. Supercritical fluid extraction of grape seed (Vitis vinifera L.) oil. Effect of the operating conditions upon oil composition and antioxidant capacity. Chem Eng J. 2010; 160(2): 634-640. doi: 10.1016/j.cej.2010.03.087
5. Sotiropoulou, E.I., Varelas, V., Liouni, M., and Nerantzis, E.T. Grape seed oil: from a winery waste to a value-added cosmetic product-a review. 2015.
6. Carmona-Jiménez Y, Igartuburu JM, Guillén-Sánchez DA, García-Moreno MV. Fatty Acid and Tocopherol Composition of Pomace and Seed Oil from Five Grape Varieties Southern Spain. Molecules. 2022; 27(20): 6980. doi:10.3390/molecules27206980
7. Sumaiyah, M. Leisyah B. The Effect of Antioxidant of Grapeseed Oil As Skin Anti-aging in Nanoemulsion and Emulsion Preparations. Rasayan J Chem. 2019; 12(3): 1185-1194. doi:10.31788/RJC.2019.1235337
8. Aburjai T, Natsheh FM. Plants used in cosmetics. Phytother Res. 2003;17(9): 987-1000. doi:10.1002/ptr.1363
9. Kamel BS, Dawson H, Kakuda Y. Characteristics and composition of melon and grape seed oils and cakes. J Am Oil Chem Soc. 1985; 62(5): 881-883. doi:10.1007/BF02541750
10. Patil H, Waghmare J. Harnessing the Power of Non-Conventional Vegetable Oils: A Review of Sustainable Product Development. Int J Technol. Published online September 20, 2024: 59-65. doi:10.52711/2231-3915.2024.00009
11. Febriyenti F, Suharti N, Putri RF. Formulation and Evaluation of Patchouli Oil Microemulsion and Microemulgel for Inhibit the Bacterial Growth. Res J Pharm Technol. 2022: 51-55. doi:10.52711/0974-360X.2022.00010
12. Lin TK, Zhong L, Santiago J. Anti-Inflammatory and Skin Barrier Repair Effects of Topical Application of Some Plant Oils. Int J Mol Sci. 2017; 19(1): 70. doi:10.3390/ijms19010070
13. Ansorena D, Ramírez R, Lopez De Cerain A, Azqueta A, Astiasaran I. Oxidative Stability and Genotoxic Activity of Vegetable Oils Subjected to Accelerated Oxidation and Cooking Conditions. Foods. 2023; 12(11): 2186. doi:10.3390/foods12112186
14. Gitea MA, Bungau SG, Gitea D, Pasca BM, Purza AL, Radu AF. Evaluation of the Phytochemistry–Therapeutic Activity Relationship for Grape Seeds Oil. Life. 2023; 13(1): 178. doi:10.3390/life13010178
15. Pant A, Agarwal S, Singh M. Bacteriostatic activity of Melaleuca alternifolia loaded Microemulsion targeting microbial skin infection by Topical Delivery. Res J Top Cosmet Sci. 2019; 10(2): 48. doi:10.5958/2321-5844.2019.00011.6
16. Pakkang N, Uraki Y, Koda K, Nithitanakul M, Charoensaeng A. Preparation of Water‐in‐Oil Microemulsion from the Mixtures of Castor Oil and Sunflower Oil as Makeup Remover. J Surfactants Deterg. 2018; 21(6): 809-816. doi:10.1002/jsde.12189
17. Najeebullah1 ST. Formulation and Evaluation of Herbal Makeup Remover Oil. Published online September 5, 2024. doi:10.5281/ZENODO.13695536
18. Kim EJ, Kong BJ, Kwon SS, Jang HN, Park SN. Preparation and characterization of W/O microemulsion for removal of oily make‐up cosmetics. Int J Cosmet Sci. 2014; 36(6): 606-612. doi:10.1111/ics.12163
19. Slomkowski S, Alemán JV, Gilbert RG, et al. Terminology of polymers and polymerization processes in dispersed systems (IUPAC Recommendations 2011). Pure Appl Chem. 2011; 83(12): 2229-2259. doi:10.1351/PAC-REC-10-06-03
20. Gaikwad R, Dange P, Nayakal P, Ramugade P, Pallavipatil P. Emerging Trends of Nanomaterials in Cosmeceuticals. Asian J Pharm Res. Published online March 22, 2023: 18-24. doi:10.52711/2231-5691.2023.00003
21. Choursiya S, Gawande P, Rathore A. A Review on Regulation and Need of Cosmetovigilance in India. Asian J Res Pharm Sci. 2021; 11(1): 86-91. doi:10.5958/2231-5659.2021. 00014.X
22. Gautam A, Upadhyay S. Formulation and Standardization of Polyherbal Face Wash Gel for Acne Management. Res J Pharm Technol. Published online September 28, 2022: 3931-3935. doi:10.52711/0974-360X.2022.00658
23. Bodkhe AA, Bedi RS, Upadhayay A, Kale MK. Ophthalmic Microemulsion: Formulation Design and Process Optimization. Res J Pharm Technol. 2018; 11(12): 5474. doi:10.5958/0974-360X.2018.00998.8
24. Danielsson I, Lindman B. The definition of microemulsion. Colloids Surf. 1981; 3(4): 391-392. doi:10.1016/0166-6622(81)80064-9
25. Date AA, Nagarsenker MS. Parenteral microemulsions: An overview. Int J Pharm. 2008; 355(1-2): 19-30. doi: 10.1016/j.ijpharm.2008.01.004
26. Jagtap SR, Phadtare DG, Saudagar RB. Microemulsion: A Current Review. Res J Pharm Dos Forms Technol. 2016; 8(2): 161. doi:10.5958/0975-4377.2016.00021.5
27. Lawrence MJ. Microemulsions as drug delivery vehicles. Curr Opin Colloid Interface Sci. 1996; 1(6): 826-832. doi:10.1016/S1359-0294(96)80087-2
28. N. Ande S, B. Sonone K, L. Bakal R, V. Ajmire P, S. Sawarkar H. Role of Surfactant and Co-surfactant in Microemulsion: A Review. Res J Pharm Technol. Published online October 21, 2022: 4829-4834. doi:10.52711/0974-360X.2022.00811
29. P. Sanap D, P. Sapkal N, S. Daud A. Design and Characterization of Microemulsion System for Fentanyl citrate. Res J Pharm Technol. Published online March 31, 2023: 1319-1326. doi:10.52711/0974-360X.2023.00217
30. Kumawat R, Patil H, Waghmare J. Unlocking the Potential of Hazelnut oil: Formulation and Characterization of a Novel Microemulsion for Enhanced Applications. Res J Sci Technol. Published online May 29, 2024: 119-124. doi:10.52711/2349-2988.2024.00018
31. Harshal Patil, Jyotsna Waghmare. Nanoemulsion: Current state and perspectives. Res. J. Topical and Cosmetic Sci. 2013; 4(1): 32-40.
32. Mihu, G. C., and Turtoi, M. (March 2011). Use of microemulsions in food industry. Paper of the Sibiu Alma Mater University Conference. Fifth Edition, 1, 361-368.
33. Berkman M, Güleç K. Pseudo ternary phase diagrams: a practical approach for the area and centroid calculation of stable microemulsion regions. Istanbul J Pharm. 2021; 51(1): 42-9.
34. Sumaiyah M, Leisyah B. The effect of antioxidant of grapeseed oil as skin anti-aging in nanoemulsion and emulsion preparations. Rasayan J Chem. 2019;12(03): 1185-94.
35. Muhammad Asri Abd Sisa, Rusli Daik, Ramli S. Study on the effect of oil phase and co-surfactant on microemulsion systems. Malaysian J Anal Sc. 2017; 21(6): 1409-16.
36. Boonme, P. (2007), Applications of microemulsions in cosmetics. Journal of Cosmetic Dermatology, 6: 223-228.
37. Danielsson, I., and Lindman, B. The definition of microemulsion. Colloids and Surfaces. 1981; 3(4): 391-392. https://doi.org/10.1016/0166-6622(81)80064-9.
38. Mason, T. G., Wilking, J. N., Meleson, K., Chang, C. B., and Graves, S. M. Nanoemulsions: formation, structure, and physical properties. Journal of Physics: Condensed Matter. 2006; 18: 635-666.
39. Paul, B.K., Moulik, S.P: Uses and Applications of Microemulsions, Current Science, 2001; 80 (8): 990 – 1001.
40. Patel R. Mrunali. Microemulsions: As Novel Drug Delivery Vehicle, 2007; 5. 8. Madhav. S and Gupta. D. A review on microemulsion based system. International Journal of Pharmaceutical Sciences and Research. 2011; 2(8): 1888
41. Ghosh, P.K. and Murthy R.S.R. Microemulsions: A Potential Drug Delivery System. Current Drug Delivery. 2006; 3: 167-180.
42. Aboofazeli R and Lawrence M.J. Investigations into the formation and characterization of phospholipid microemulsions. I. Pseudo-ternary phase diagrams of systems containing water- lecithin-alcohol isopropyl myristate. International Journal of Pharmaceutics. 1993; 93: 161-175.
43. Sidhu, J. S., J. Al-Saqer and S. Al-Zenki, 1996. Comparison of methods for the assessment of the extent of staling in bread. Food Chemistry. 58: 161-168.
44. Yemis, O., E. Bakkalbasi and N. Artik, 2008. Antioxidative activities of grape (Vitis vinifera) seed extracts obtained from different varieties grown in Turkey. International Journal of Food Science and Technology. 43: 154-159.
45. Mildner-Szkudlarz, S., R. Zawirska, A. Szwengiel and M. Pacyňski,. Use of grape by-product as a source of dietry fibre and phenolic compounds in sourdough mixed rye bread. International Journal of Food Science and Technology. 2011; 46: 1485-1493.
46. Talegaonkar S, Azeem A, Ahmad FJ, Khar RK, Pathan SA, Khan ZI. Microemulsions: a novel approach to enhanced drug delivery. Recent Pat Drug Deliv Formul. 2008; 2(3): 238-57
47. Lourith, N., and Kanlayavattanakul, M. Development of para rubber seed oil as the efficient makeup remover. Brazilian Journal of Pharmaceutical Sciences. 2020; 56: e18029. https://doi.org/10.1590/s2175-97902019000418029.
48. Panithi Raknam, Sirirat Pinsuwan and Thanaporn Amnuaikit, Rubber Seed Cleansing Oil Formulation and its Efficacy of Makeup Remover. IJPSR. 2020; 11(1): 146-155. DOI: 10.13040/IJPSR.0975-8232.11(1).146-55
49. Sonam Pandey, Prerana Sahu, Divyansh Sahu, Harish Sharma, Gyanesh Kumar Sahu. Efficiency and Safety of Makeup Removal Using Castor Oil and Olive Oil Combination. IJIRT. 2024; 10(1).