Author(s):
Sayali D. Waghchoure, Nilima S. Dharkar, Asmita A. Wele
Email(s):
asmita.wele@gmail.com
DOI:
10.52711/0974-360X.2026.00493 
Address:
Sayali D. Waghchoure1, Nilima S. Dharkar2, Asmita A. Wele3*
1Department of Rasashastra and Bhaishajya Kalpana, Dr. D. Y. Patil College of Ayurved and Research Centre, Pimpri, Pune - 411018, Maharashtra, India.
2Department of Rasashastra and Bhaishajya Kalpana, Dr. D. Y. Patil College of Ayurved and Research Centre, Pimpri, Pune - 411018, Maharashtra, India.
3Department of Rasashastra and Bhaishajya Kalpana, Dr. D. Y. Patil College of Ayurved and Research Centre, Pimpri, Pune - 411018, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 8,
Year - 2026
ABSTRACT:
Background: To treat Melasma, a mask of Laksha (Laccifer lacca) resin and Shirisha (Albizia lebbeck) seeds is recommended. The permeation of the actives from mask has limitations due to particle size. Objectives: To formulate a LS nanogel using Laksha (Laccifer lacca) resin and Shirisha (Albizia lebbeck) seeds and to assess the short term/accelerated stability of LS nanogel. Method: Aqueous extracts of Laccifer lacca resin and Albizia lebbeck seeds using Soxhlet’s apparatus were extracted. A 100mL stock solution of AgNO3 was prepared, and the extracts of both drugs were mixed with the prepared AgNO3 stock solution in 1:9 ratio. A LS decoction of Laccifer lacca resin and Albizia lebbeck seeds was prepared following Ayurvedic guidelines and divided into three glass containers. Carbopol 940p, DMDM, glycerin, and the activated AgNO3-solution were added to the decoction. After allowing the gelling agent to swell for 1–2 hours, vigorous stirring was performed to achieve a gel-like consistency. Triethanolamine (TEA) was then added to neutralize the samples. Total six pilot batches were prepared and sample from three batches sent for accelerated stability study. Results: Short term stability study for 30 days: 4°C±2°C (cold storage), 25°C±2°C (room temperature), and 40°C±2°C (accelerated conditions) temperature conditions showed that LS nanogel maintained consistent visual appearance, colour, odour, state, pH, and viscosity over a month. These results indicate that the preformulated products from Batch 1, 2 and 3 preserved its quality without degradation, confirming a good shelf life for 30 days. Conclusion: A standard operating procedure (SOP) was developed for formulating a Laksha-Shirisha (LS) nanogel using Laccifer lacca resin and Albizia lebbeck seed. Furthermore, accelerated stability test was performed to evaluate the product's quality and shelf life.
Cite this article:
Sayali D. Waghchoure, Nilima S. Dharkar, Asmita A. Wele. Development and Accelerated Stability Assessment of Laksha-Shirisha (LS) Nanogel. Research Journal of Pharmacy and Technology. 2026;19(8):3482-8. doi: 10.52711/0974-360X.2026.00493
Cite(Electronic):
Sayali D. Waghchoure, Nilima S. Dharkar, Asmita A. Wele. Development and Accelerated Stability Assessment of Laksha-Shirisha (LS) Nanogel. Research Journal of Pharmacy and Technology. 2026;19(8):3482-8. doi: 10.52711/0974-360X.2026.00493 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-8-10
REFERENCES:
1. Sindhu RK, Gupta R, Wadhera G, Kumar P. Modern Herbal Nanogels: Formulation, Delivery Methods, and Applications. Gels. 2022 Feb 7; 8(2): 97. doi: 10.3390/gels8020097. PMID: 35200478; PMCID: PMC8872030.
2. Molina M., Asadian-Birjand M., Balach J., Bergueiro J., Miceli E., Calderón M. Stimuli-responsive nanogel composites and their application in nanomedicine. Chem. Soc. Rev. 2015; 44: 6161–6186. doi: 10.1039/C5CS00199D
3. Suryawanshi NR, Nagoba SN, Agarkar P, Chavan R, Patil R, Mehtre P, et al. Design, development and evaluation of herbal nanogel containing Achyranthes aspera. Res J Pharm Technol. 2025; 18(7): 2961-6. doi:10.52711/0974-360X.2025.00424.
4. Natarajan T, Prasanth KG, Mahendran S, Varghese JV, Gupta DPK, Sneha VR, et al. Development and in vivo evaluation of nanogel drug delivery system for promoting wound healing in diabetic induced rats. Res J Pharm Technol. 2024; 17(8): 3807-13. doi:10.52711/0974-360X.2024.00591.
5. Kabanov AV, Vinogradov SV. Nanogels as pharmaceutical carriers: finite networks of infinite capabilities. Angew Chem Int Ed Engl. 2009; 48(30): 5418–29. doi:10.1002/anie.200900441
6. Solanki SS, Patel KB, Patel JG, Patel MP, Patel JK. Transdermal drug delivery systems: a review. Res J Pharm Technol. 2012; 5(6): 757-63
7. Nahas E, Fakhry G, Shereen et al. Effect of various penetration enhancers on diclofenac sodium. Asian J Pharm. 2011; 5: 33.
8. Government of India, Ministry of AYUSH. The Ayurvedic Pharmacopoeia of India. Part I, Vol. I. New Delhi: Department of Indian Systems of Medicine and Homoeopathy; 2001. p. Appendix–2.1, General Guidelines for Churna
9. Government of India, Ministry of AYUSH. The Ayurvedic Formulary of India. Part I. 2nd ed. New Delhi: Department of AYUSH; 2003. p. Appendix–3, Preparation of Lepa and Churna.
10. Deshmukh B, Dubey S, Pandey RK, Shukla SS. Development of quality control parameter of Hingwashtak Churna. Res J Pharm Technol. 2018; 11(4): 1319-26. doi:10.5958/0974-360X.2018.00246.9.
11. Indian Pharmacopoeia Commission. Indian Pharmacopoeia 2022. Ghaziabad: IPC; 2022
12. Usharani B. Extraction of essential oils from Cymbopogon citratus using organic solvents. Res J Pharm Technol. 2021; 14(11): 5709-12. doi:10.52711/0974-360X.2021.00992
13. World Health Organization. WHO Guidelines on Good Agricultural and Collection Practices (GACP) for Medicinal Plants. Geneva: WHO; 2003. p. 38.
14. Indian Pharmacopoeia Commission. Indian Pharmacopoeia 2022. Vol I. Ghaziabad: IPC; 2022. p. A-95
15. Ahmed S, Saifullah, Ahmad M, Swami BL, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci. 2016; 9(1): 1–7. doi:10.1016/j.jrras.2015.06.006
16. Sharangadhara. Sharangadhara Samhita, translated by Srikantha Murthy KR. 6th ed. Varanasi: Chaukhambha Orientalia; 2006. Madhyama Khanda, Chapter 2, Verses 1–4.
17. Panda P, Das B. A comprehensive review of Ayurvedic pharmaceutical dosage forms: historical perspectives and modern innovations. Res J Pharm Technol. 2025; 18(4): 1931-6. doi:10.52711/0974-360X.2025.00276.
18. Mukherjee S, Maity S, Ghosh B, Mondal A. Accelerated stability study of preformulated glyburide loaded lyophilized lipid nanoparticles. Res J Pharm Technol. 2020; 13(7): 3323-5. doi:10.5958/0974-360X.2020.00589.2
19. Ministry of AYUSH. Guidelines for Stability Study of Ayurvedic, Siddha and Unani Drugs. New Delhi: Department of AYUSH, Government of India; 2008.) (World Health Organization. Stability Testing of Active Pharmaceutical Ingredients and Finished Pharmaceutical Products. WHO Technical Report Series, No. 1010, Annex 10. Geneva: WHO; 2018
20. Ministry of AYUSH. The Ayurvedic Pharmacopoeia of India. Part I, Vol I–VII. New Delhi: Government of India, Ministry of AYUSH; 2001–2022.
21. (Bureau of Indian Standards. IS 5402:2012 – Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the Enumeration of Microorganisms – Colony Count Technique at 30°C. New Delhi: BIS; 2012.)
22. Nair R, Sevukarajan M. Formulation and evaluation of silver nanoparticles incorporated herbal gel for antimicrobial activity. Asian J Pharm Clin Res. 2013;6(Suppl 3):186–90.
23. Singh P, et al. Formulation Development and Investigations on Therapeutic Potential of Nanogel from Beta vulgaris L. Extract in Testosterone Induced Alopecia. PMC. 2023
24. Hamzah ML. Formulation and evaluation of flurbiprofen nanogel. Res J Pharm Technol. 2020; 13(11): 5183-8. doi:10.5958/0974-360X.2020.00906.3.
25. Maheshwari S, Tiwari RK, Singh L. Green expertise: synthesis of silver nanoparticles for wound healing application – an overview. Res J Pharm Technol. 2021; 14(2): 1149-54. doi:10.5958/0974-360X.2021.00206.7
26. Bose S, Shinde H, Karikalan K, Lalitha P, Mandal AKA. Antibacterial, anti-inflammatory, and antiproliferative activity of silver nanoparticles synthesized from leaf extract of Azadirachta indica A. Juss. Res J Pharm Technol. 2016; 9(12): 2422-6. doi:10.5958/0974-360X.2016.00483.2
27. Rowe RC, Sheskey PJ, Quinn ME, editors. Handbook of Pharmaceutical Excipients. 6th ed. London: Pharmaceutical Press; 2009. p. 109–111
28. Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem. 2011;13(10):2638–50. doi:10.1039/C1GC15386B
29. Singh P, Kim YJ, Zhang D, Yang DC. Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol. 2016;34(7):588–99. doi:10.1016/j.tibtech.2016.02.006
30. Mizińska K, et al. Formulation and Evaluation of Nanogels of Tapinarof for Psoriasis. Gels. 2024; 10(11): 675. doi:10.3390/gels10110675
31. Verma A, Singh S, Kumari A, Pathak K. Development and evaluation of herbal carbopol gel containing extracts of Curcuma longa and Aloe vera. Pharmacogn J. 2017; 9(3): 350–6. doi:10.5530/pj.2017.3.58
32. Rowe RC, Sheskey PJ, Quinn ME, editors. Handbook of Pharmaceutical Excipients. 6th ed. London: Pharmaceutical Press; 2009. p. 109–111.
33. Mudgil P, Dhabhai R, Chourasia A, Mishra G, Chourasia MK. Formulation development and evaluation of a nanogel from Beta vulgaris L. extract for testosterone-induced alopecia. Pharmaceutics. 2023; 15(1): 100–114.
34. Nair R, Sevukarajan M. Formulation and evaluation of silver nanoparticles incorporated herbal gel for antimicrobial activity. Asian J Pharm Clin Res. 2013; 6(Suppl 3): 186–90.