Author(s):
Pradip P. Bawane, Santosh Yele
Email(s):
santoshyele@gmail.com
DOI:
10.52711/0974-360X.2026.00425 
Address:
Pradip P. Bawane1,2, Santosh Yele2*
1Department of Pharmacognosy, SVKM's NMIMS, Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, Mumbai, 400056, India.
2Department of Pharmacognosy, Shri Vile Parle Kelavani Mandal's Institute of Pharmacy, Dhule, India – 424001.
3Department of Pharmacognosy, SVKM's NMIMS, School of Pharmacy & Technology Management, Telangana, Hyderabad, 509301, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 7,
Year - 2026
ABSTRACT:
Background: Lonar Soda Lake, formed in basaltic rock by a meteorite impact, possesses unique geochemical conditions that support microorganisms with distinct metabolic capabilities, including antibiotic, biosurfactant, and biofuel production. Exploring the genetic basis of these capabilities can uncover novel compounds and biotechnological innovations. Aim: To analyze metagenomic sequences from bacterial communities in Lonar Lake to identify genetic signatures associated with antibiotic production, biosurfactant synthesis, and biofuel pathways. Methods: Metagenomic DNA was extracted from Lonar Lake samples and sequenced using high-throughput techniques. Bioinformatic analyses identified genes related to antibiotic production, biosurfactant synthesis, and biofuel pathways. DNA extraction was performed using the Qiagen gDNA kit and quantified with the Qubit dsDNA HS Assay kit. 16S rRNA gene sequencing libraries were prepared with the Ion 16S Metagenomics Kit and sequenced on the Ion Torrent S5 system. Results: Metagenomic analysis revealed a highly diverse bacterial community, comprising 19 phyla. Dominant phyla included Proteobacteria (48.12%), Firmicutes (26.44%), and Actinobacteria (0.98%). We identified 31 Actinomycetes families and 161 species, including industrially significant genera such as Pseudonocardia, Saccharomonospora, Actinomadura, Amycolatopsis, Nocardia, and Saccharopolyspora. Notable species included Mycobacterium salmoniphilum, Microbispora rosea, and Nocardiopsis alba. Genomic signatures for antibiotic production, biosurfactant synthesis, and biofuel pathways were identified. Conclusion: The metagenomic analysis of Lonar Lake bacterial communities highlights their potential for novel antimicrobial, antiviral, biosurfactant, and biofuel production, offering promising avenues for biotechnological applications and industrial wastewater treatment.
Cite this article:
Pradip P. Bawane, Santosh Yele. Exploration of Metagenomic Sequences Derived from Bacterial Assemblages in the Hypersaline Lonar Lake of India: Revelation of Distinctive Genomic Signatures Encoding Pharmaceutical Significance. Research Journal of Pharmacy and Technology. 2026;19(7):2985-1. doi: 10.52711/0974-360X.2026.00425
Cite(Electronic):
Pradip P. Bawane, Santosh Yele. Exploration of Metagenomic Sequences Derived from Bacterial Assemblages in the Hypersaline Lonar Lake of India: Revelation of Distinctive Genomic Signatures Encoding Pharmaceutical Significance. Research Journal of Pharmacy and Technology. 2026;19(7):2985-1. doi: 10.52711/0974-360X.2026.00425 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-7-12
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