Author(s):
Fathia Kesuma Dinanti, Mae Sri Hartati Wahyuningsih, Marsetyawan Soesatyo, Jajah Fachiroh, Ary Setya Hernanda, Benny Diah Madusari
Email(s):
fathiakesumadinanti@mail.ugm.ac.id
DOI:
10.52711/0974-360X.2026.00406 
Address:
Fathia Kesuma Dinanti1,2*, Mae Sri Hartati Wahyuningsih3,4, Marsetyawan Soesatyo5, Jajah Fachiroh5, Ary Setya Hernanda6, Benny Diah Madusari7
1Doctoral Program of Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Sleman Regency, 55284, Yogyakarta, Indonesia.
2Department of Biology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, 50112, Central Java, Indonesia.
3Center for Herbal Medicine, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Sleman Regency, 55284, Yogyakarta, Indonesia.
4Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Sleman Regency, 55284, Yogyakarta, Indonesia.
5Department of Histology and Cell Biology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Sleman Regency, 55284, Yogyakarta, Indonesia.
6Department of Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115, East Java, Indone
Published In:
Volume - 19,
Issue - 6,
Year - 2026
ABSTRACT:
Mangrove plants grow widely along coastal areas in Sigandu mangrove forest, Indonesia and are often used as medicinal plants by the society, but they remain scientifically underexplored. This is the first study which assess the bioactive compounds, antioxidant, and anti-inflammation activity of mangrove plants from Sigandu, Indonesia. We identified 5 mangrove species based on their morphology, including Rhizophora apiculata, Rhizophora mucronata, Avicennia alba, Avicennia marina, and Bruguiera gymnorrhiza. Mangrove leaves were extracted using ethanol 90%. The extracts were analyzed through phytochemical screening. The DPPH (1,1-diphenyl-2-picrylhydrazil) assay was used to evaluate their antioxidant potential. The anti-inflammatory potential was assessed by examining its impacts on carrageenan-decrease paw edema in rats. Interleukin (IL)-1ß and tumor necrosis factor (TNF)-a concentrations in the inflamed paw were determined using ELISA kits. The findings showed that all extracts contained flavonoids, phenolics, terpenoids, tannin, and only Rhizophora apiculata did not contain saponin compounds. The antioxidant activity of five extracts were very strong, with Bruguiera gymnorrhiza being the strongest with IC50 value of 6.74 µg/mL. In vivo study showed that Avicennia alba provided the most significantly different (p<0.05) paw edema thickness compared to the negative control. Similarly, we found that five species of mangrove leave significantly lowered the concentration of pro-inflammatory cytokines. Moreover, Avicennia alba at dosage 400 mg/kg BW possessed the ability to reduce the concentration of TNF-a inside the inflamed tissue as effective as ibuprofen as drug standard. Thus, the ethanolic extracts of mangrove leaves from Sigandu are potential source of antioxidant and anti-inflammatory agents, which may be caused by secondary metabolites such as flavonoid, phenolics, terpenoids, and tannin.
Cite this article:
Fathia Kesuma Dinanti, Mae Sri Hartati Wahyuningsih, Marsetyawan Soesatyo, Jajah Fachiroh, Ary Setya Hernanda, Benny Diah Madusari. Phytochemical Analysis, Antioxidant Activity, and Anti-Inflammatory Potential of Mangrove Leaves from Sigandu, Indonesia. Research Journal Pharmacy and Technology. 2026;19(6):2843-2. doi: 10.52711/0974-360X.2026.00406
Cite(Electronic):
Fathia Kesuma Dinanti, Mae Sri Hartati Wahyuningsih, Marsetyawan Soesatyo, Jajah Fachiroh, Ary Setya Hernanda, Benny Diah Madusari. Phytochemical Analysis, Antioxidant Activity, and Anti-Inflammatory Potential of Mangrove Leaves from Sigandu, Indonesia. Research Journal Pharmacy and Technology. 2026;19(6):2843-2. doi: 10.52711/0974-360X.2026.00406 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-6-63
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