Author(s):
Rupinder Kaur, Kanchan Chatterjee, Preeti Handa, Versha Upadhyay, Tanuja, Simrat Kaur
Email(s):
roopnarang@gmail.com
DOI:
10.52711/0974-360X.2026.00279 
Address:
Rupinder Kaur1*, Kanchan Chatterjee2, Preeti Handa3, Versha Upadhyay4, Tanuja5, Simrat Kaur6
1Department of Agriculture, Tula’s Institute, Dehradun-248001, Uttarakhand, India.
2Department of Zoology, Dev Bhoomi Uttarakhand University, Dehradun-248001, Uttarakhand, India.
3School of Agriculture, Dev Bhoomi Uttarakhand University, Dehradun-248001, Uttarakhand, India.
4Department of Botany, Maya Devi University, Dehradun-248001, Uttarakhand, India.
5Department of Applied Science and Humanities, Tula’s Institute, Dehradun-248001, Uttarakhand, India.
6Department of Chemistry, Mata Gujri College, Fatehgarh Sahib-140406, Punjab, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 4,
Year - 2026
ABSTRACT:
Neem, scientifically known as Azadirachta indica (A. Juss.) is an evergreen, temperature-resistant flowering tree native to India, though it is now found across various continents. Famous for its wide range of therapeutic uses, almost all parts of the neem tree are used in both contemporary pharmaceutical formulations and traditional medicine. Neem is effective against a range of microorganisms and ectoparasites, including bacteria, ticks, viruses, fungi, viruses, ticks, and mites. Additionally, it offers benefits such as antimalarial, neuroprotective, anticancer, antioxidant, antidiabetic, hepatoprotective, and wound-healing properties. Beyond its medicinal uses, neem is also employed in agriculture as a pesticide and fertilizer to boost crop yields. Despite its many advantages, research and reviews on neem, particularly in India, have not been as thorough as those on other medicinal plants. To fully explore its potential, there is a need for more extensive research and development, supported by government funding to advance studies on this remarkable tree.
Cite this article:
Rupinder Kaur, Kanchan Chatterjee, Preeti Handa, Versha Upadhyay, Tanuja, Simrat Kaur. Bio-active Components and Medicinal Properties of Azadirachta indica (A. Juss.): A Comprehensive Review. Research Journal of Pharmacy and Technology. 2026;19(4):1946-2. doi: 10.52711/0974-360X.2026.00279
Cite(Electronic):
Rupinder Kaur, Kanchan Chatterjee, Preeti Handa, Versha Upadhyay, Tanuja, Simrat Kaur. Bio-active Components and Medicinal Properties of Azadirachta indica (A. Juss.): A Comprehensive Review. Research Journal of Pharmacy and Technology. 2026;19(4):1946-2. doi: 10.52711/0974-360X.2026.00279 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-4-68
REFERENCES:
1. Anonymous. Neem- Growing neem, organic farming, health, animal health, environmental use, home uses, economic potential, patents, new eJBiio Electronic. 2006. https://www.arcjournals.org/journal-of-immunology-and-vaccines/volume-2-issue-2/4
2. Fathima SK. Investigations on the biology and management of Phomopsis azadirachtae on neem. Ph.D. thesis, University of Mysore, Mysore, India. 2004. https://www.researchgate.net/publication/264657693_Phomopsis_azadirachtae_-_The_Die-Back_of_Neem_Pathogen
3. Hegde NG. Neem and small farmers constraints at grass root level. Indian Forester. 1995; 121(11): 1040-1048. https://mail.i-scholar.in/index.php/indianforester/article/view/7374/0
4. Biswas K. Chattopadhyay I. Banerjee RK. Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Current Science. 2002; 82: 1336-1345. https://www.jstor.org/stable/24106000
5. Subapriya R. Nagini S. Medicinal properties of neem leaves: a review. Curr. Med. Chem. Anticancer Agents. 2005; 5: 149-160. DOI: 10.2174/1568011053174828
6. Verkerk R. Wright D. Biological activity of neem seed kernel extract and synthetic azadirachtin against larvae of Plutella xylostellal, Pesticide Science. 1993; 37: 83-91. https://imskolkata.org/article1/015-03-23.pdf
7. Parotta JA. Healing plants of Peninsular India., New York, CABI Publishing 2001; 495-96. https://www.scirp.org/reference/referencespapers?referenceid=1671390
8. Ali A. Textbook of Pharmacognosy. New Delhi, India: Publication and Information Directorate. 1993. https://www.sciepub.com/reference/38183
9. Kokate CK. Purohit AP. Gokhale SB. Text book of Pharmacognosy. Published by Nirali Prakashan. 2010. https://www.amazon.in/Pharmacognosy-Dr-C-K-Kokate-ebook/dp/B07JHNNMWB
10. Govindachari TR. Suresh G. Gopalakrishnan G. Banumathy B. Masilamani S. Identification of antifungal compounds from the seed oil of Azadirachta indica. Phytoparasitica. 1998; 26(2): 109-116. https://link.springer.com/article/10.1007/BF02980677
11. Naik MR. Bhattacharya A. Behera R. Agrawal D. Dehury S. Kumar S. Study of anti-inflammatory effect of neem seed oil (Azadirachta indica) on infected albino rats. Journal of Health Research and Reviews (In Developing Countries). 2014; 1(3): 66-69. DOI: 10.4103/2394-2010.153880
12. Schumacher M. Cerella C. Reuter S. Dicato M. Diederich M. Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway. Genes & Nutrition. 2011; 6: 149-160. https://doi.org/10.1007/s12263-010-0194-6
13. Batista FLA. Lima LM. Abrante IA. de Araújo JIF. Batista FLA. Abrante IA. Magalhaes FEA. Antinociceptive activity of ethanolic extract of Azadirachta indica A. Juss (Neem, Meliaceae) fruit through opioid, glutamatergic and acid-sensitive ion pathways in adult zebrafish (Danio rerio). Biomedicine & Pharmacotherapy. 2018; 108: 408-416. DOI: 10.1016/j.biopha.2018.08.160
14. Prakash A. Tewari R. Mathur R. Non-hormonal post-coital contraceptive action of Neem oil in rats. J Ethnopharmacol. 2006; 23(1): 53-59. DOI: 10.1016/0378-8741(88)90114-6
15. Brototi B. Kaplay RD. Azadirachta indica (Neem): It’s Economic utility and chances for commercial planned plantation in Nanded District. International Journal of Pharm. 2011; 1(2): 100-104. https://www.pharmascholars.com/archive/ijp-volume-1-issue-2-year-2011.html
16. Ross IA. Medicinal plants of the world: Chemical constituents, Traditional and modern medicinal uses, Totowa, New Jersy. 2001; 2: 81-85. http://ndl.ethernet.edu.et/bitstream/123456789/44034/1/63.Ivan%20A.%20Ross.pdf
17. Noorul A. Gayathri. Beneficial effects of Neem oil-An updated review. Journal of Pharm. Sci. and Res. 2012; 8(8): 756-758. https://www.jpsr.pharmainfo.in/Documents/Volumes/vol8Issue08/jpsr08081613.pdf
18. Sharma P. Tomar L. Bachwani M. Bansal V. Review on Neem (Azadirachta indica): Thousand Problem One Solution, International Res. J. of Pharmacy. 2011; 2(12): 97-102. https://www.researchgate.net/publication/288971662_Review_on_neem_Azadirachta_indica_Thousand_problems_one_solution
19. Dora imefonudoh. Naomi udo. Asamudo. Bal DN. Enwongo O. Inhibitory effect of varying concentrations of leaves extracts of Centella asiatica (gotu kola) on some microorganisms of medical importance. 2012; 3(2): 142-148. https://www.researchgate.net/publication/372189792_Inhibitory_Effect_of_Varying_Concentrations_of_Leaves'_Extracts_of_Centella_Asiatica_Gotu_Kola_on_Some_Microorganisms_of_Medical_Importance
20. Siddiqui BS. Afshan F. Gulzar T. Tetracyclic triterpenoids from the leaves of Azadirachta indica. Phytochemistry. 2004; 65: 2363-2367. DOI: 10.1016/j.phytochem.2004.04.031
21. Khillare B. Shrivastav TG. Spermicidal activity of Azadirachta indica (neem) leaf extract. Contraception., 2003; 68: 225-229. DOI: 10.1016/s0010-7824(03)00165-3
22. Ascher KRS. Nonconventional insecticidal effects of pesticides available from the neem tree, Azadirachta indica. Archives of Insect Biochemistry and Physiology. 1993; 22: 433-449. https://doi.org/10.1002/arch.940220311
23. Koul O. Isman MB. Ketkar CM. Properties and uses of neem, Azadirachta indica. Can. J. of Botany. 1987; 68:1-11. https://doi.org/10.1139/b90-001
24. Gomase PV. Rangari VD. Verma PR. Phytochemical evaluation and hepatoprotective activity of fresh juice of young stem (tender) bark of Azadirachta indica A. Juss. International Journal of Phar and Pharma Science. 2011; 3(2): 55-59. https://www.innovareacademics.in/journal/ijpps/Vol3Suppl2/994.pdf
25. Mahfuzul Hoque MD. Bari ML. Inatsu Y. Juneja VK. Kawamoto S. Antibacterial activity of guava (Psidium guajava L.) and neem (Azadirachta indica A. Juss.) extracts against food borne pathogens and spoilage bacteria, Food borne Pathogens and Disease. 2007; 4(4): 481-488. DOI: 10.1089/fpd.2007.0040
26. Yerima M. Jodi S. Oyinbo K. Maishanu H. Farouq A. Junaidu AU. Effect of neem extracts (Azadirachta indica) on bacteria isolated from adult mouth. Journal of Basic and Appl. Sci. 2012; 20: 64-67. https://www.ajol.info/index.php/njbas/article/view/81588
27. Chava VR. Manjunath SM. Rajanikanth AV. Sridevi N. The efficacy of neem extract on four microorganisms responsible for causing dental caries viz Streptococcus mutans, Streptococcus salivarius, Streptococcus mitis and Streptococcus sanguis: an in vitro study. J. of Contemp. Dent. Pract. 2012; 13(6): 769-772. DOI: 10.5005/jp-journals-10024-1227
28. Vanwyk BE. Wink M. Medicinal Plants of the world, Times Editions, Briza Publications, Arcadia. 2004; 64. https://www.scirp.org/reference/referencespapers?referenceid=2178431
29. Saradhajyothi K. Subbarao B. Antimicrobial potential of the extracts of the leave of Azadirachta indica, Linn. Natural Sci. Biol. 2011; 3(1): 65-69. https://doi.org/10.15835/nsb315470
30. Shravan KD. Ramakrishna R. Santhosh KM. Kannappan N. In vivo Antidiabetic evaluation of Neem leaf extract in alloxan induced rats. Journal of Applied Pharma. Science. 2011; 1(4): 100-105. https://japsonline.com/abstract.php?article_id=68&sts=2
31. Mondali NK. Mojumdar A. Chatterje SK. Banerjee A. Datta JK. Gupta S. Antifungal activities and chemical characterization of neem leaf extract on the growth of some selected fungal species in vitro culture medium. Journal of Applied Science and Env. Manag. 2009; 13(1): 49-53. https://www.bioline.org.br/pdf?ja09008
32. Aarati N. Ranganath N. Soumya G. Kishore B. Mithun K. Evaluation of Antibacterial and Anticandidal efficacy of aqueous and alcoholic extract of Neem (Azadirachta indica) an in vitro study. International Journal of Res. in Ayurveda & Pharmacy. 2011; 2(1): 230-235. https://www.researchgate.net/publication/289220639_Evaluation_of_antibacterial_and_anti_candidial_efficay_of_aqueous_and_alcoholic_extract_of_Neem_Azadirachta_indica_-_an_in_vitro_study
33. Tiwari V. Darmani NA. Yue BY. Shukla D. In vitro antiviral activity of neem (Azadirachta indica L.) bark extract against herpes simplex virus type-1 infection. Phyto. Res., 2010; 24(8): 1132-1140. DOI: 10.1002/ptr.3085
34. Bhowmik D. Chiranjib. Yadav J. Tripathi K. Kumar K. Herbal remedies of Azadirachta indica and its medicinal application. Journal of Chemical and Pharmaceutical Research. 2010; 2(1): 62-67. https://www.jocpr.com/articles/herbal-remedies-of-azadirachta-indica-and-its-medicinal-application.pdf
35. Mbah AU. Udeinya IJ. Shu EN. Chijioke CP. Nubila T. Udeinya F. Fractionated neem leaf extract is safe and increases CD4+ cell levels in HIV/AIDS patients, Am. J. Ther. 2007; 14(4): 369-374. DOI: 10.1097/MJT.0b013e3180a72199
36. Ben-Erick Van Wyk. Michael Wink. Medicinal Plants of the world, Times Editions, Singapore. 2004; pp. 64. https://books.google.co.in/books/about/Medicinal_Plants_of_the_World.html?id=KC-_AQAACAAJ&redir_esc=y
37. Sateesh MK. Microbiological investigations on die-back disease of neem (Azadirachta indica A. Juss.). Ph.D. thesis. University of Mysore. Mysore, India. 1998. https://www.researchgate.net/publication/264657693_Phomopsis_azadirachtae_-_The_Die-Back_of_Neem_Pathogen
38. Nunes XP. Silva FS. Guedes JR. Almeida S. Biological oxidations and antioxidant activity of natural products, in Phytochemicals as Nutraceuticals-Global Approaches to Their Role in Nutrition and Health, In Tech. 2012. DOI:10.5772/26956
39. Rahmani AH. Aly SM. Nigella sativa and its active constituents thymoquinone shows pivotal role in the disease’s prevention and treatment. Asian J. of Pharma. and Cli. Res. 2015; 8(1): 48-53. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=a9130fa459bdb68d9a44bad0bcf4023dd5b8e218
40. Priyadarsini RV. Manikandan P. Kumar GH. Nagini S. The neem limonoids azadirachtin and nimbolide inhibit hamster cheek pouch carcinogenesis by modulating xenobiotic metabolizing enzymes, DNA damage, antioxidants, invasion and angiogenesis. Free Radical Research. 2009; 43(5): 492-504. DOI: 10.1080/10715760902870637
41. Lavanya U. Garge VN. Kadam VJ. Evaluation of anti-angiogenesis activity of neem root using zebra fish model. International Journal of Pharma. Science and Research. 2015; 6(6): 2437-2440. DOI: 10.13040/IJPSR.0975-8232.6(6).2437-40
42. Osunwoke EA. Olotu EJ. Allison T. Onyekwere J. The wound healing effects of aqueous leave extracts of Azadirachta indica on wistar rats. Journal of Natural Science and Res. 2013;3(6). https://core.ac.uk/download/pdf/234654141.pdf
43. El-Hawary Z. Kholief T. Antifertility effect of neem. Archives of Pharmacology and Research. 1990; 13: 108-112. doi:10.1016/j.jep.2004.05.011
44. Chattopadhyay RR. Chattopadhyay RN. Nandy AK. Poddar G. Maitra SK. Preliminary report on antihyperglycemic effect of fraction of fresh leaves of Azadiracta indica (Beng neem). Bull. of the Calcutta. Sch. of Trop. Med. 1987a; 35: 29-32. https://www.researchgate.net/publication/352507719_Antihyperglycemic_effects_of_Azadirachta_indica_Neem_An_overview