Author(s):
Suhas P. Padmane, Prajakta G. Patekar, Jyotiram A. Sawale
Email(s):
suhaspadmane@yahoo.co.in
DOI:
10.52711/0974-360X.2026.00263 
Address:
Suhas P. Padmane1*, Prajakta G. Patekar1, Jyotiram A. Sawale2
1Gurunanak College of Pharmacy, Mauza-Nari, Near Dixit Nagar, Kamptee Road, Nagpur - 440026, Maharashtra State, India.
2Department of Pharmacognosy, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth, Karad - 415539, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 4,
Year - 2026
ABSTRACT:
A derivative UV-spectrophotometric method has been developed and validated for estimation of ozenoxacin in bulk drug and cream formulations. The standard solution of ozenoxacin in 0.1N HCl was prepared and wavelength 245nm was selected for method development. The third order derivative spectrum was selected for determination of ozenoxacin in presence of preservative benzoic acid where the latter shows zero absorption at 245 nm. The linearity of the method was found in range 5-25µg/ml in 0.1N HCl with regression value of 0.9996. The recovery of ozenoxacin was found to be around 100%, indicating accuracy of the method. The method was found to be precise with maximum RSD of 1.176%. The LOD and LOQ were found to be 0.568µg/ml and 1.721µg/ml respectively. The developed method was specific for the estimation of ozenoxacin in the presence of preservative benzoic acid in cream formulation, and can be effectively used for routine quality control in industry.
Cite this article:
Suhas P. Padmane, Prajakta G. Patekar, Jyotiram A. Sawale. Novel Third Order Derivative UV-Spectrophotometric Method and Validation for Estimation of Ozenoxacin in Cream Formulations. Research Journal of Pharmacy and Technology. 2026;19(4):1837-1. doi: 10.52711/0974-360X.2026.00263
Cite(Electronic):
Suhas P. Padmane, Prajakta G. Patekar, Jyotiram A. Sawale. Novel Third Order Derivative UV-Spectrophotometric Method and Validation for Estimation of Ozenoxacin in Cream Formulations. Research Journal of Pharmacy and Technology. 2026;19(4):1837-1. doi: 10.52711/0974-360X.2026.00263 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-4-52
6. REFERENCES:
1. Available at https://go.drugbank.com/drugs/DB12924.
2. Wren C, Bell E, Eiland LS. Ozenoxacin: a novel topical quinolone for impetigo. Annals of Pharmacotherapy. 2018 Dec; 52(12): 1233-7.
3. Ramireddy AR, Behara DK. Analytical method validation on simultaneous estimation of Ozenoxacin and Benzoic acid in pharmaceutical formulation. Acta Chromatographica. 2023 Jul 18; 35(3): 278-85.
4. Ameya L. Stability indicating assay method (Siam) for the estimation of ozenoxacin using RP-HPLC method. Asian Journal of Pharmaceutical Research and Development. 2024 May; 12(4): 58-68.
5. Santos B, Ortiz J, Gropper S. In vitro percutaneous absorption and metabolism of ozenoxacin in excised human skin. Future Microbiology. 2014 Aug 1; 9(sup8): S3-9.
6. Ibrahim AE, Taher MF, El Gindy A, Ibrahim EA. Novel green HPTLC and organic solvent-free micellar LC methods for the simultaneous determination of Ozenoxacin and benzoic acid; greenness assessment and applications. Sustainable Chemistry and Pharmacy. 2023 Dec 1; 36: 101277.
7. Mali AD, Manojkumar P. Estimation of ranitidine in bulk and formulation by first order derivative area under curve UV-spectrophotometry methods. Asian Journal of Pharmaceutical Analysis. 2015; 5(2): 61-6.
8. Mali A, Kolekar S, Panachery J, Tamboli A. Simultaneous determination of paracetamol and domperidone in pharmaceutical dosage form by first order derivative UV spectrophotometry. Asian Journal of Pharmceutical Research. 2016 Jan; 6(1): 22-6.
9. Kumaraswamy G, Lalitha R, Kumar DS. Validated first order derivative spectrophotometric method for simultaneous estimation of lansoprazole and aspirin in tablet dosage forms. Asian Journal of Research in Pharmaceutical Science. 2016 Sep 1; 6(3): 185-190.
10. Aaraf TH, Raj HA, Jain VC, Sutariya V. Development and validation of ratio derivative spectrophotometric method for estimation of metronidazole benzoate and related impurity in bulk and pharmaceutical formulation. Asian Journal of Pharmacy and Technology. 2015; 5(2): 66-70.
11. Thamake SL, Jadhav SD, Pishawikar SA. Development and validation of method for simultaneous estimation of atorvastatin calcium and ramipril from capsule dosage form by first order derivative spectroscopy. Asian Journal of Research in Chemistry. 2009; 2(1): 52-3.
12. Bhatia NM, Gavali AY. Development and validation of method for simultaneous estimation of atenolol and lercanidipine from tablet dosage form by second order derivative spectroscopy. Asian Journal of Research in Chemistry. 2009; 2(4): 398-400.
13. Bhadoriya AS, Padekar SS, Patil VD, Shinde SN, Choudhari VP, Kuchekar BS. Development and validation of ratio spectra derivative spectrophotometric method for determination of ternary mixture of Aspirin, Atenolol and Amlodipine Besylate in formulation. Asian Journal of Research in Chemistry. 2010; 3(3): 562-5.
14. Anusha T, Sri RS. Derivative UV spectroscopic cleaning method development and validation of darunavir API incorporated into dissolving oral film. Research Journal of Science and Technology. 2024; 16(2): 107-14.
15. Singh M, Charde M, Shukla R, Charde RM. Determination of calcipotriene in calcipotriene cream 0.05% w/w by RP-HPLC method development and validation. Research Journal of Pharmacy and Technology. 2011; 4(8): 1219-23.
16. Nanda RK, Kulkarni AA, Ranjane MN, Ranjane PN. A stability-indicating HPTLC method for estimation of nadifloxacin in topical cream. Research Journal of Topical and Cosmetic Sciences. 2010; 1(1): 25-9.
17. Available at https://pubchem.ncbi.nlm.nih.gov/compound/ Benzoic-acid. Accessed on 22 August, 2025.
18. Issa HM, Mohammed DH. A critical review on the journey of benzoic acid in the pharmaceutical industry from manufacturing processes through various uses to disposal: An environmental perspective. Environmental Analysis, Health and Toxicology. 2025 Mar 5; 40: e2025007.
19. Roy J. An introduction to pharmaceutical sciences- production, chemistry, techniques and technology. Woodhead Publishing, 2011 Jul, 170.
20. Kar M, Chourasiya Y, Maheshwari R, Tekade RK. Current developments in excipient science: implication of quantitative selection of each excipient in product development. InBasic Fundamentals of Drug Delivery 2019 Jan 1 (pp. 29-83). Academic Press.
21. ICH Q2 (R1): Validation of Analytical Procedures: Text and Methodology. In International Conference on Harmonization, Geneva 2005 Nov 6 (Vol. 2005).