ABSTRACT:
This work seeks to develop and validate a straightforward, rapid, economical, sensitive, and extractive spectrophotometric technique for the quantification of bromocriptine mesylate (BMC) in both pure form and pharmaceutical formulations. The devised methods rely on the production of ion-pair complexes between bromocriptine mesylate and three dyes: bromocresol purple (BCP), methyl orange (MO), and alizarin red S (ARS) in acidic buffer solutions. The various parameters influencing the response between bromocriptine mesylate and the dyes were examined and optimized. The synthesized complexes were extracted using methylene chloride and analyzed at 409, 424, and 428nm with BCP, MO, and ARS, respectively. Beer’s law was adhered to within the ranges of 1.0–16, 1.0–24, and 1.0–20µg mL-1 while employing BCP, MO, and ARS, respectively, under optimal conditions. The ion pairs were determined to have a 1:1 composition. The molar absorptivity, Sandell’s sensitivity, and the limits of detection and quantification were computed. Additional method validation metrics, including intra-day and inter-day accuracy and precision, robustness, ruggedness, and selectivity, have been assessed. The proposed methodologies have been effectively utilized for the analysis of bromocriptine mesylate in both its pure form and pharmaceutical formulations. The methods' reliability was further confirmed through recovery trials utilizing the usual addition technique. A statistical comparison of the results with the reported approach was conducted using Student's t-test and F-test, revealing no significant statistical differences.