Author(s):
G. M. Srimyvizhiy, K. B. Ilango, Prince Paulraj. W, Prakash K, Navaneethakrishnan. S, Edvin Sherin. S, Nila. S. S, Padmavathi. P, Pragathi. S, Sindhu. R, Pooja. M
Email(s):
princebieber28@gmail.com
DOI:
10.52711/0974-360X.2026.00216 
Address:
G. M. Srimyvizhiy1*, K. B. Ilango2, Prince Paulraj. W3, Prakash K4, Navaneethakrishnan. S5, Edvin Sherin. S6, Nila. S. S7, Padmavathi. P8, Pragathi. S9, Sindhu. R10, Pooja. M11
1Department of Pharmaceutical Analysis, Shree Venkateshwara College of Paramedical Sciences, Othakuthirai, Gobi, Erode, Tamil Nadu, India. Affiliated to the Tamil Nadu Dr. M.G.R. Medical University, Chennai, India.
2Department of Pharmaceutics, Shree Venkateshwara College of Paramedical Sciences, Othakuthirai, Gobi, Erode, Tamil Nadu, India. Affiliated to the Tamil Nadu Dr. M.G.R. Medical University, Chennai, India.
3-11B. Pharm Final Year, Shree Venkateshwara College of Paramedical Sciences, Othakuthirai, Gobi, Erode, Tamil Nadu, India. Affiliated to the Tamil Nadu Dr. M.G.R. Medical University, Chennai, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 4,
Year - 2026
ABSTRACT:
Quercetin, a flavonoid widely present in fruits, vegetables, and medicinal plants, exhibits potent antioxidant, anti-inflammatory, antimicrobial, and cardiovascular protective effects. This study aimed to develop and validate a simple, rapid, sensitive, and cost-effective UV-visible spectrophotometric method for the quantification of quercetin in marketed Amla tablets. Standard and sample solutions were prepared and scanned at 256 nm, which was identified as the ?max. The method was validated according to ICH Q2(R1) guidelines for specificity, linearity (2–10 µg/mL, R² = 0.9993), accuracy (99.81–100.7% recovery), precision (%RSD <2%), LOD (0.206 µg/mL), and LOQ (0.624 µg/mL). The proposed method showed no interference from excipients, demonstrated robustness, and was successfully applied to commercially available Amla tablets. The method can be used for routine quality control and estimation of quercetin in pharmaceutical formulations.
Cite this article:
G. M. Srimyvizhiy, K. B. Ilango, Prince Paulraj. W, Prakash K, Navaneethakrishnan. S, Edvin Sherin. S, Nila. S. S, Padmavathi. P, Pragathi. S, Sindhu. R, Pooja. M. Development and Validation of a UV Spectrophotometric Method for the Quantification of Quercetin in Amla Tablets. Research Journal of Pharmacy and Technology. 2026;19(4):1506-0. doi: 10.52711/0974-360X.2026.00216
Cite(Electronic):
G. M. Srimyvizhiy, K. B. Ilango, Prince Paulraj. W, Prakash K, Navaneethakrishnan. S, Edvin Sherin. S, Nila. S. S, Padmavathi. P, Pragathi. S, Sindhu. R, Pooja. M. Development and Validation of a UV Spectrophotometric Method for the Quantification of Quercetin in Amla Tablets. Research Journal of Pharmacy and Technology. 2026;19(4):1506-0. doi: 10.52711/0974-360X.2026.00216 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-4-5
REFERENCES:
1. Sharma S, Arora V. Simultaneous spectrophotometric estimation of curcumin and quercetin in mixture. Res J Pharm Technol. 2022; 15(8): 3502-3506. doi:10.52711/0974-360X.2022.00587.
2. Garg S, Chahal R, Kaushik D, Kumar R, Mittal V. RP-HPLC method development and validation for simultaneous estimation of rutin and quercetin in Morus alba L. leaf extract. Res J Pharm Technol. 2023; 16(5): 2327-2332. doi:10.52711/0974-360X.2023.00383.
3. Wahdaningsih S, Rizkifani S, Untari EK, Yap I. Qualitative and quantitative analysis of quercetin from Hylocereus polyrhizus peel methanol extract by HPTLC. Res J Pharm Technol. 2025; 18(1): 63-66. doi:10.52711/0974-360X.2025.00010.
4. Bachri M, Nasution LR, Ginting MB. Extraction and determination of quercetin and ascorbic acid simultaneously in tomatoes (Solanum lycopersicum L.) using mean centering of ratio spectra. Res J Pharm Technol. 2025; 18(2): 823-828. doi:10.52711/0974-360X.2025.00122.
5. Daharwal SJ, Shrivastava S. HPTLC method development and validation for quantification of quercetin in Thuja occidentalis mother tincture. Res J Pharm Technol. 2019; 12(9): 4102-4106. doi:10.5958/0974-360X.2019.00707.8.
6. Chaurasia U, Soni V, Sahu RK. Separation of gallic acid and quercetin from Cassia tora extract using thin layer chromatography: simple and cost-effective approach. Res J Pharm Technol. 2024; 17(10): 4847-4850. doi:10.52711/0974-360X.2024.00745.
7. Shrivastava AK, Verma S, Awasthi H. HPTLC phytochemical profiling and simultaneous quantification of quercetin and gallic acid in Prosopis juliflora (Sw.). Res J Pharm Technol. 2024; 17(8): 3801-3806. doi:10.52711/0974-360X.2024.00590.
8. Pinisetti D, Kakadiya J, Patel AB. Role of quercetin as an effective bioenhancer in curcumin absorption: in vitro study. Res J Pharm Technol. 2022; 15(11): 4867-4870. doi:10.52711/0974-360X.2022.00817.
9. Ngamkhae N, Chulikhit Y, Monthakantirat O, et al. Development and validation of HPLC method for simultaneous determination of five active compounds in Kleeb Bua Daeng formula. Res J Pharm Technol. 2022; 15(8): 3618-3626. doi:10.52711/0974-360X.2022.00606.
10. Wisudyaningsih B, Sallama S, Setyawan D, Siswandono. The effect of pH and cocrystal quercetin-isonicotinamide on solubility enhancement. Res J Pharm Technol. 2021; 14(9): 4231-4235. doi:10.5958/0974-360X.2021.00791.0.
11. Surve RR, Alegaon S, Patil S, et al. Marker-based standardization of quercetin in marketed herbal formulations using novel UV-spectrophotometric methods. Indian J Pharm Educ Res. 2023; 57(3 Suppl): S772-S785.
12. Thakkar M, Sharma S. Development and validation of UV-visible spectrophotometric method for the estimation of quercetin in herbal dosage forms. Int J Chem Sci. 2019; 17(1): 1-8.
13. Patidar M, Gopkumar P, Sridevi G, Behera CC, Pillai S. Development and validation of RP-HPLC method for simultaneous determination of resveratrol and curcumin in pure form. Res J Pharm Technol. 2013; 6(9): 990-992.
14. Sahu S, Kumari K, Muduli NR, Moharana AK. Development of UV spectrophotometry absorption correction method for estimation of curcumin and aspirin from bulk. Res J Pharm Technol. 2019; 12(10): 4857-4860.
15. Nagarnaik M, Sarjoshi A, Bodkhe A, Khanal B, Pise M, Pandya G. Characterization of active constituents in turmeric powder and validation of method for curcumin in samples. Asian J Res Chem. 2015; 8(10): 643-647.
16. Rathod SD, Patil PM, Jadhav SB, et al. UV spectrophotometric simultaneous determination of metformin hydrochloride and pioglitazone in combined dosage forms. Asian J Pharm Anal. 2012; 2(1): 5-9.
17. Jumle RS, Mundhey AS, Wate SP, Dangare SS, Ramteke UD. UV-spectrophotometric method development and validation for estimation of tizanidine and aceclofenac in tablet formulation. Asian J Pharm Anal. 2012; 2(4): 101-103.
18. Jain M, Yogesh A, Chavhan R, Bari M. UV spectrophotometric methods for simultaneous estimation of levosulpiride and esomeprazole in capsule dosage form. Asian J Pharm Anal. 2012; 2(4): 106-109.
19. Daharwal SJ. Development and validation of UV spectrophotometric methods for simultaneous estimation of diazepam and propranolol in bulk drug and its formulation. Asian J Pharm Anal. 2013; 3(1): 20-23.
20. Gupta SK, Kumar B, Sharma PK. Development and validation of a spectrophotometric method for estimation of triamcinolone in solid dosage form. Asian J Pharm Anal. 2013; 3(2): 42-43.
21. Santhi N, Rajendran SS, Nallasivan PK, et al. Simultaneous estimation of cefixime and ofloxacin in bulk and tablet dosage form. Asian J Pharm Anal. 2011; 1(3): 50-52.
22. Sharma YR. Ultraviolet and visible spectroscopy. In: Sharma YR, editor. Elementary Organic Spectroscopy. 1st ed. New Delhi: S. Chand and Co. Ltd.; 2004. p. 9-60.
23. Willard HH, Merritt LL, Dean J, Frank AS. Instrumental Methods of Analysis. 7th ed. New Delhi: CBS Publishers and Distributors; 1986. p. 185-190.
24. Verma G, Mishra DR. Development and optimization of UV-Vis spectroscopy – a review. World J Pharm Res. 2018; 7(11): 1170-1180.
25. Skoog DA, Holler FJ, Nieman DA. Introduction to UV spectroscopy. In: Skoog DA, Holler FJ, Nieman DA, editors. Principles of Instrumental Analysis. 5th ed. Cole Publication; 2004. p. 215-218.
26. Gandhimathi R, Vijayaraj S, Jyothirmaie MP. Analytical process of drugs by ultraviolet (UV) spectroscopy – a review. Int J Pharm Res Anal. 2012; 2(2): 72-78.
27. Lakhanpal P, Rai DK. Quercetin: a versatile flavonoid. Internet J Med Update. 2007; 2(2): 22-37.
28. Aghababaei F, Hadidi M. Recent advances in potential health benefits of quercetin. Pharmaceuticals. 2023; 16(7): 1020.
29. Al-Shakarchi W, Abdulaziz NT, Mustafa YF. A review of the chemical, pharmacokinetic, and pharmacological aspects of quercetin. Eurasian Chem Commun. 2022; 4(7): 645-656.
30. Shabir I, Pandey VK, Shams R, Dar AH, Dash KK, Khan SA, et al. Promising bioactive properties of quercetin for potential food applications and health benefits: a review. Front Nutr. 2022; 9: 999752.
31. Shetti PP, Farheen H, Kubade MY, Gurao RP. Development and validation of UV-visible spectrophotometric method for estimation of quercetin. J Chem Health Risks. 2024; 14(4): 1784-1792.
32. Patil V, Angadi S, Devdhe S. Determination of quercetin by UV spectroscopy as a quality control parameter in herbal plant Cocculus hirsutus. J Chem Pharm Res. 2015;7(1):99-104.
33. Duan Y. Ultraviolet-visible spectrum characterizations of quercetin in aqueous ethanol solution with different pH values. J Chem Pharm Res. 2014; 6(9): 236-240.
34. Askal HF, Saleh GA, Backheet EY. A selective spectrophotometric method for determination of quercetin in the presence of other flavonoids. Talanta. 1992; 39(3): 259-263.
35. Thakkar M, Sharma S. Method optimization and wavelength selection for UV-visible spectrophotometric estimation of quercetin in herbal dosage forms. Int J Chem Sci. 2019; 17(1): 1-8.
36. Shetti PP, Gurao RP. Validation of UV method for quercetin analysis in marketed herbal formulations. J Chem Health Risks. 2024; 14(4): 1784-1792.
37. Surve RR, Patil S. Standardization of quercetin in herbal tablets using UV-visible spectroscopy. Indian J Pharm Educ Res. 2023; 57(3 Suppl): S772-S785.
38. Patil V, Angadi S. Solvent selection and method validation for quercetin in plant formulations. J Chem Pharm Res. 2015; 7(1): 99-104.
39. Duan Y. Effect of pH on UV-visible spectrum and λmax of quercetin in aqueous ethanol solutions. J Chem Pharm Res. 2014; 6(9): 236-240.
40. Askal HF, Saleh GA. Selectivity of UV-visible method for quercetin in presence of flavonoids. Talanta. 1992; 39(3): 259-263.
41. Thakkar M, Sharma S. Precision and accuracy assessment in UV spectrophotometric method for quercetin. Int J Chem Sci. 2019;17(1):1-8.
Shetti PP, Farheen H. Application of validated UV-visible method for marketed Amla tablet analysis. J Chem Health Risks. 2024; 14(4): 1784-1792.