Author(s):
Madina Amangeldinova, Nataliya Kudrina, Gulzira Yernazarova, Svetlana Turasheva, Metin Yildirim, Aliya Ramazanova, Gulnur Admanova, Gulnarai Sabdenalieva, Gulnur Omarova
Email(s):
yernazarova.gulzira@kaznu.kz
DOI:
10.52711/0974-360X.2026.00146 
Address:
Madina Amangeldinova1,2, Nataliya Kudrina1,2, Gulzira Yernazarova3, Svetlana Turasheva4, Metin Yildirim5, Aliya Ramazanova6, Gulnur Admanova7, Gulnarai Sabdenalieva8, Gulnur Omarova9
1Al Farabi Kazakh National University, Almaty 050040, Republic of Kazakhstan.
2Institute of Genetics and Physiology, Almaty 050040, Republic of Kazakhstan.
1Al Farabi Kazakh National University, Almaty 050040, Republic of Kazakhstan.
2Institute of Genetics and Physiology, Almaty 050040, Republic of Kazakhstan.
3Al Farabi Kazakh National University, Almaty 050040, Republic of Kazakhstan.
4Al Farabi Kazakh National University, Almaty 050040, Republic of Kazakhstan
5Department of Biochemistry, Faculty of Pharmacy, Cukurova University, Adana 01330, Turkey.
6Kazakh National Women's Teacher Training University, Almaty 050000, Republic of Kazakhstan.
7K. Zhybanov Aktobe Regional University, Aktobe 030000, Republic of Kazakhstan.
8Abai Kazakh National Pedagogical University, Almaty 050010, Repu
Published In:
Volume - 19,
Issue - 3,
Year - 2026
ABSTRACT:
The increasing demand for novel antimicrobial agents has led to interest in plant-derived bioactive substances (BAS). This study focuses on the establishment of callus cultures from Rheum cordatum Losinsk. and Rheum tataricum L. under in vitro conditions and the evaluation of the antimicrobial properties of BAS synthesized in these callus cells. Callus cultures were induced from leaf and stem explants using Murashige-Skoog nutrient medium supplemented with 2,4-D (0.1mg/L) and BAP (0.01mg/L). Callus formation frequency was recorded for both species, and green plant regeneration from R. cordatum calluses was observed. Antimicrobial activity was assessed by testing callus-derived extracts against various microorganisms, including gram-positive Staphylococcus aureus 6538 and Salmonella abony NTCC 6017, gram-negative Escherichia coli NTCC 8439 and Klebsiella pneumonia 700603, and the yeast-like fungus Candida albicans R50. Callus induction was successfully achieved in both species, with formation frequencies of 60±6% for R. cordatum and 72±8% for R. tataricum. Extracts from R. tataricum callus cultures exhibited antimicrobial activity against S. aureus, E. coli, and K. pneumoniae. R. cordatum callus extracts showed moderate effects on these microorganisms. The results highlight the significant potential of callus-derived rhubarb extracts in addressing bacterial and fungal pathogens. The study demonstrates the efficacy of Murashige-Skoog medium supplemented with 2,4-D and BAP in promoting callus induction and growth in rhubarb species. Extracts from rhubarb callus tissues represent significant potential in developing new antimicrobial agents effective against a wide range of pathogens, including bacterial and fungal infections. Future studies will focus on the regeneration of plants from callus cultures and the detailed pharmacological profiling of their BAS.
Cite this article:
Madina Amangeldinova, Nataliya Kudrina, Gulzira Yernazarova, Svetlana Turasheva, Metin Yildirim, Aliya Ramazanova, Gulnur Admanova, Gulnarai Sabdenalieva, Gulnur Omarova. Investigation of the Antimicrobial Activity of Biologically Active Substances Extracted from the Callus Cell Cultures of Rheum cordatum Losinsk and Rheum tataricum L. Plants (Family Polygonaceae) using Fourier infrared Spectroscopy to Analyze the Chemical Composition and Identify Active Compounds. Research Journal Pharmacy and Technology. 2026;19(3):1033-1. doi: 10.52711/0974-360X.2026.00146
Cite(Electronic):
Madina Amangeldinova, Nataliya Kudrina, Gulzira Yernazarova, Svetlana Turasheva, Metin Yildirim, Aliya Ramazanova, Gulnur Admanova, Gulnarai Sabdenalieva, Gulnur Omarova. Investigation of the Antimicrobial Activity of Biologically Active Substances Extracted from the Callus Cell Cultures of Rheum cordatum Losinsk and Rheum tataricum L. Plants (Family Polygonaceae) using Fourier infrared Spectroscopy to Analyze the Chemical Composition and Identify Active Compounds. Research Journal Pharmacy and Technology. 2026;19(3):1033-1. doi: 10.52711/0974-360X.2026.00146 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-3-7
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