Author(s):
Rajendra. O. Ganjiwale, Deorao. M. Awari
Email(s):
dev_awari@rediffmail.com
DOI:
10.52711/0974-360X.2026.00158 
Address:
Rajendra. O. Ganjiwale1,2, Deorao. M. Awari1,2*
1Institute of Pharmaceutical Education and Research, Borgaon Meghe, Wardha, Maharashtra, India.
2Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 3,
Year - 2026
ABSTRACT:
Background: The researcher reported that phenolic and flavonoid compounds are the major contributors to antioxidant activity, acting through the inhibition of enzymes responsible for increasing oxidative stress. Objectives: The present study aimed to evaluate the in vitro antioxidant activity of the bio-guided fraction of Artemisia pallens by the DPPH method. Methods: The bio-guided fractionation of the ethanolic extract was done using Hexane, Chloroform, Ethyl acetate, and Water. The phytochemical investigation, qualitative and quantitative analysis, and Chromatographic analysis were done. The IC50 value was calculated by the regression method. Result: The phytochemical analysis of Artemisia pallens reveals the presence of flavonoids, phenolic compounds, glycosides, terpenoids, and alkaloids in different solvent fractions. Ethyl acetate and water fractions showed significant activity (IC50 = 171.17 and IC50 = 16.76µg/ml, respectively). The water fraction, having a low IC50 value (16.76µg/mL) indicates more antioxidant activity, but the ethyl acetate fraction, having a higher IC50 (171.17µg/mL), was selected as an effective fraction for further analysis due to its moderate polarity and feasible extraction yield, . Though the water fraction showed potent in vitro activity, in contrast, the highly polar constituents of the water fraction may exhibit limited absorption and bioavailability in vivo. lipophilic fractions exhibited superior in vivo antioxidant effects compared to highly polar fractions, despite weaker in vitro performance. Conclusion: The phytochemical analysis and in-vitro DPPH assay of the ethyl acetate fraction showed promising antioxidant activity, probably due to the higher concentration of flavonoids and phenolic compounds. Therefore, the ethyl acetate fraction was considered more suitable for antioxidant activity, as it represents bioactive constituents capable of effective in vivo antioxidant defence rather than radical scavenging. Therefore, these findings support that Artemisia pallens may be useful as an option for the management of stress as an antioxidant agent. Further in-vivo studies are needed to correlate antioxidant potential with possible neuroprotective outcomes.
Cite this article:
Rajendra. O. Ganjiwale, Deorao. M. Awari. In-vitro Antioxidant Activity of Bio-guided Fraction of Artemisia pallens. Research Journal Pharmacy and Technology. 2026;19(3):1118-4. doi: 10.52711/0974-360X.2026.00158
Cite(Electronic):
Rajendra. O. Ganjiwale, Deorao. M. Awari. In-vitro Antioxidant Activity of Bio-guided Fraction of Artemisia pallens. Research Journal Pharmacy and Technology. 2026;19(3):1118-4. doi: 10.52711/0974-360X.2026.00158 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-3-19
REFERENCES:
1. Suresh J, Kumar P, Ramesh C, Meena V. Phytochemical and pharmacological properties of Artemisia pallens. International Journal of Pharmaceutical Sciences and Research. 2011; 2(12): 3081–3088.
2. Sharma S, Rana M, Kumar H, Parashar B. It’s era to move towards nature for getting beneficial effects of plants having antioxidant activity to fight against deleterious diseases. Asian Journal of Pharmaceutical Research. 2013; 3(2): 103–106.
3. Jaiswal YS, Williams LL. A glimpse of Ayurveda—the forgotten history and principles of Indian traditional medicine. Journal of Traditional and Complementary Medicine. 2017; 7(1): 50–53.
4. Alakararao GS, Prasad JG, Rajendra Y. Investigations on the antifungal activity of the essential oil from Artemisia pallens and Artemisia vulgaris Linn. Indian Perfumer. 1981; 2: 112–114.
5. Government of India. The Ayurvedic Pharmacopoeia of India. Part I, Vol. V. New Delhi: Ministry of Health and Family Welfare, Department of AYUSH; 2001: 210.
6. Pandey AK, Singh P. The genus Artemisia: A literature review on chemical composition, antimicrobial, insecticidal and antioxidant activities of essential oils. Medicines. 2017; 4(3): 68.
7. Praveen N, Murthy HN, Hahn EJ, Paek KY. Production of bioactive compounds from cell suspension cultures of Artemisia pallens. Plant Cell Tissue and Organ Culture. 2016; 126(1): 39–50.
8. Das S, Dey A, Ghosh S. Fractionation and phytochemical screening of plant extracts: Recent advances. Journal of Pharmacognosy and Phytochemistry. 2019; 8(3): 3000–3008.
9. Singh R, Kumar N, Singh S. Evaluation of antioxidant potential and phytochemical analysis of different solvent fractions of medicinal plants. Indian Journal of Experimental Biology. 2020; 58(5): 327–333.
10. Sharma P, Gupta R, Verma A. Bioassay-guided fractionation and antioxidant activity of medicinal plant extracts. Journal of Pharmacology Research International. 2021; 33(2): 45–52.
11. Rangasamy P, Hansiya VS, Maheswari PU, Suman T, Geetha N. Phytochemical analysis and in-vitro antioxidant and anti-urolithiatic potential of Clitoria ternatea. Asian Journal of Pharmaceutical Analysis. 2019; 9(2): 67–76.
12. Kondragunta VK, Karuppuraj V, Perumal K. Antioxidant activity and folic acid content in indigenous isolates of Ganoderma lucidum. Asian Journal of Pharmaceutical Analysis. 2016; 6(4): 213–215.
13. Samal PK. Antioxidant activity of Strobilanthes asperrimus in albino rats. Asian Journal of Pharmaceutical Research. 2013; 3(2): 71–74.
14. Madhu C, Swapna J, Neelima K, Shah MV. Comparative evaluation of antioxidant activity of some medicinal plants used in India. Asian Journal of Research in Pharmaceutical Sciences. 2012; 2(3): 98–100.
15. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998: 60–66.
16. Trease GE, Evans WC. Pharmacognosy. 16th ed. Edinburgh: Saunders Elsevier; 2009: 134–148.
17. Patel DS, Shah PB, Managoli NB. In-vitro antioxidant and free radical scavenging activities of Withania somnifera and Aloe vera. Asian Journal of Pharmaceutical Technology. 2012; 2(4): 143–147.
18. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols by Folin–Ciocalteu reagent. Methods in Enzymology. 1999; 299: 152–178.
19. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content by colorimetric methods. Journal of Food and Drug Analysis. 2002; 10(3): 178–182.
20. Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography Atlas. 2nd ed. Berlin: Springer-Verlag. 1996: 195–210.
21. Blois MS. Antioxidant determinations using a stable free radical. Nature. 1958; 181: 1199–1200.
22. Abriyani E, Fikayuniar L. Phytochemical screening, antioxidant activity and vitamin C assay of Begonia versicolar. Asian Journal of Pharmaceutical Research. 2020; 10(3): 183–187.
23. Ashfaq MH, Siddique A, Shahid S. Antioxidant activity of Cinnamomum zeylanicum: A review. Asian Journal of Pharmaceutical Research. 2021; 11(2): 106–110.
24. Brand-Williams W, Cuvelier ME, Berset C. Free radical method to evaluate antioxidant activity. LWT–Food Science and Technology. 1995; 28(1): 25–30.
25. Boots AW, Haenen GR, Bast A. Health effects of quercetin. European Journal of Pharmacology. 2008; 585(2–3): 325–337.
26. Hasni S, Khelil A, Habita S, et al. Effect of feeding system on antioxidant activity of camel urine. Asian Journal of Pharmaceutical Research. 2022; 12(4): 261–266.
27. Malathi R, Ahamed John S, Cholarajan A. Antioxidant activity of Tylophora asthmatica leaves. Asian Journal of Research in Pharmaceutical Sciences. 2012; 2(2): 80–82.
28. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry and metabolism. Journal of Nutritional Biochemistry. 2002; 13(10): 572–584.
29. Pacher P, Nivorozhkin A, Szabó C. Therapeutic effects of xanthine oxidase inhibitors. Pharmacological Reviews. 2006; 58(1): 87–114.
30. Okamoto K, Eger BT, Nishino T, et al. Crystal structure of xanthine oxidoreductase–allopurinol complex. Journal of Biological Chemistry. 2004; 279(32): 31759–31762.
31. Trott O, Olson AJ. AutoDock Vina: Improving docking speed and accuracy. Journal of Computational Chemistry. 2010; 31(2): 455–461.
32. Pettersen EF, Goddard TD, Huang CC, et al. UCSF Chimera—a visualization system. Journal of Computational Chemistry. 2004; 25(13): 1605–1612.
33. Prior RL, Wu X, Schaich K. Standardized methods for antioxidant capacity determination. Journal of Agricultural and Food Chemistry. 2005; 53(10): 4290–4302.
34. Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: Antioxidants and beyond. American Journal of Clinical Nutrition. 2002; 81(1 Suppl): 215S–217S.
35. Castro-e-Silva JH, et al. Pharmacological potential of flavonoids against neurotropic viruses. Pharmaceuticals. 2022; 15(9): 1149.
36. Aruoma OI. Free radicals and antioxidants in human health. Journal of the American Oil Chemists’ Society. 1998; 75(2): 199–212.
37. Pietta PG. Flavonoids as antioxidants. Journal of Natural Products. 2000; 63(7): 1035–1042.
38. Saha MR, Hasan SMR, Akter R, et al. In-vitro free radical scavenging activity of Terminalia chebula. Pharmacologyonline. 2008; 3: 440–449.