Author(s):
Sneha Patil, Divya Parsekar, Pranali Mehta, Akash Jakkannavar
Email(s):
snehabpatil11@gmail.com
DOI:
10.52711/0974-360X.2026.00120 
Address:
Sneha Patil1*, Divya Parsekar2, Pranali Mehta2, Akash Jakkannavar3
1Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India-590010.
2Department of Pharmaceutical Quality Assurance, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Belagavi, Karnataka, India-590010
3Department of Pharmacognosy, JSS College of Pharmacy, Mysore, JSS Academy of Higher Education and Resaerch, Mysore , Karnataka, India - 570015
*Corresponding Author
Published In:
Volume - 19,
Issue - 2,
Year - 2026
ABSTRACT:
The present study aims to produce and validate easy, accurate, precise, inexpensive, rapid and sensitive UV-spectrophotometric technique to determine Berberine and Gallic acid simultaneously in polyherbal churna. Method was developed and validated in terms of LOD, LOQ, robustness, accuracy and roughness. The developed method was used for determination of Berberine and Gallic acid in polyherbal churna. The maximum absorbance of the drugs was found at 229nm for Berberine and 217nm for Gallic acid, respectively. The linearity response concentration range (10-50µg/mL) of Berberine and Gallic acid was observed with ( Y = 1.9577x +0.0184, r2 =0.998 and Y = 1.8506x +0.0028) respectively. The accuracy was found between 98.25% and 101.9%. The precision for intra and interday measurements were found to be within the limits. LOD and LOQ were calculated to determine the method's sensitivity and the values were found to be 0.014µg/mL and 0.042µg/mL for berberine, gallic acid's values were found to be 0.012µg/mL and 0.038µg/mL, respectively. The suggested approach has been verified and can be applied to the regular examination of Berberine and Gallic acid in pharmaceutical dosage forms.
Cite this article:
Sneha Patil, Divya Parsekar, Pranali Mehta, Akash Jakkannavar. Simultaneous UV-Spectrophotometric Method for Quantifying Berberine and Gallic Acid in Polyherbal Formulation and Evaluation of its Antioxidant Potential. Research Journal of Pharmacy and Technology. 2026;19(2):845-0. doi: 10.52711/0974-360X.2026.00120
Cite(Electronic):
Sneha Patil, Divya Parsekar, Pranali Mehta, Akash Jakkannavar. Simultaneous UV-Spectrophotometric Method for Quantifying Berberine and Gallic Acid in Polyherbal Formulation and Evaluation of its Antioxidant Potential. Research Journal of Pharmacy and Technology. 2026;19(2):845-0. doi: 10.52711/0974-360X.2026.00120 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-2-49
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