Author(s):
Bhagya Lakshmi E, Sugishna M, Sreejesh Pilakkavil Chirakkara
Email(s):
pc.sreejesh@gmail.com
DOI:
10.52711/0974-360X.2026.00118 
Address:
Bhagya Lakshmi E, Sugishna M, Sreejesh Pilakkavil Chirakkara*
School of Lifesciences, Department of Biotechnology, St Aloysius Deemed to be University, Mangalore - 575003, Karnataka, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 2,
Year - 2026
ABSTRACT:
This study explores the green synthesis of silver nanoparticles (AgNPs) using methanolic bark extract from Holigarna arnottiana, aiming to develop an eco-friendly and efficient method for nanoparticle production. The synthesis was rapidly achieved within 2 h by optimizing key parameters, including silver nitrate concentration, extract concentration, and pH. The resulting H. arnottiana mediated silver nanoparticles (HAgNPs) were characterized using UV-Vis spectroscopy, Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-Ray Diffraction (XRD), confirming their spherical shape, crystalline structure, and an average particle size of approximately 77.27 ± 1.14 nm. Antioxidant activity of the HAgNPs was evaluated through the DPPH free radical scavenging assay, showing significant activity with an IC50 value of 232.33 µg/mL. Their anticancer potential was assessed using the MTT assay on PA-1 human ovarian teratocarcinoma cells, revealing a dose-dependent cytotoxic effect with an IC50 value of 216.03 µg/mL. These bioactivities are attributed to the presence of phytochemicals such as glycosides, terpenoids, and carbohydrates in the bark extract, which play key roles in reducing silver ions and stabilizing the nanoparticles. FTIR analysis further supports the involvement of these compounds in nanoparticle formation and biological activity. Overall, this work highlights the potential of H. arnottiana as a valuable source for the green synthesis of bioactive silver nanoparticles, with promising applications in antioxidant and anticancer therapies. The findings provide a foundation for future research into plant-based nanomaterials for biomedical applications.
Cite this article:
Bhagya Lakshmi E, Sugishna M, Sreejesh Pilakkavil Chirakkara. The Healing Paradox: Exploiting Poisonous Holigarna arnottiana Bark for Green-Synthesized Antioxidant and Anticancer Silver Nanoparticles. Research Journal of Pharmacy and Technology. 2026;19(2):827-4. doi: 10.52711/0974-360X.2026.00118
Cite(Electronic):
Bhagya Lakshmi E, Sugishna M, Sreejesh Pilakkavil Chirakkara. The Healing Paradox: Exploiting Poisonous Holigarna arnottiana Bark for Green-Synthesized Antioxidant and Anticancer Silver Nanoparticles. Research Journal of Pharmacy and Technology. 2026;19(2):827-4. doi: 10.52711/0974-360X.2026.00118 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-2-47
REFERENCES:
1. Yetisgin AA, Cetinel S, Zuvin M, et al. Therapeutic nanoparticles and their targeted delivery applications. Molecules. 2020; 25(9): 2193. Doi: 10.3390/molecules25092193.
2. Balram, Navneet Kaur, Kamal, Gurvirender Singh, Deepika Aggarwal. Nanotechnology in herbal drug delivery systems: enhancing therapeutic efficacy and patient compliance. Research Journal of Pharmacy and Technology. 2024; 17(2): 934-8. Doi: 10.52711/0974-360X.2024.00145
3. Yadav S, Raman APS, Singh MB, et al. Green nanoparticles for advanced corrosion protection: Current perspectives and future prospects. Applied Surface Science Advances. 2024; 21: 100605. Doi: 10.1016/j.apsadv.2024.100605.
4. Lokapur V, Jayakar V, Shantaram M. Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species-endemic plant species of western ghats. Biomedicine (India) 2020; 40(4): 460-466. Doi: /10.51248/.v40i4.319.
5. Manilal A, Idhayadhulla A. Potential in vitro antimicrobial efficacy of Holigarna arnottiana (Hook F). Asian Pac J Trop Biomed. 2014; 4(1): 25-9. Doi: 10.1016/S2221.
6. Ajaykumar AP, Sabira O, Binitha VS, et al. Bio-fabricated silver nanoparticles from the leaf extract of the poisonous plant, Holigarna arnottiana: Assessment of antimicrobial, antimitotic, anticancer, and radical-scavenging properties. Pharmaceutics. 2023; 15. Doi: 10.3390/pharmaceutics15102468.
7. Srinivasan M, Chandane AY, Tagalpallewar AA, et al. Kerala’s anticancer flora: A comprehensive review. J Appl Pharm Sci. 2024; 14(09): 018–045. Doi:10.7324/JAPS.2024.180248.
8. Ramezani T, Nabiuni M, Baharara J, et al. Sensitization of resistance ovarian cancer cells to cisplatin by biogenic synthesized silver nanoparticles through p53 activation. Iran J Pharm Res. 2019; 18(1): 222-231.Doi:https://doi.org/10.22037/ijpr.2019.2358.
9. Ajith S, Almomani F, Elhissi A, et al. Nanoparticle-based materials in anticancer drug delivery: Current and future prospects. Heliyon 2023; 9: e21227. Doi: 10.1016/j.heliyon.2023.e21227.
10. Karande KM, Gawade SP. Synthesis of nanosilver and its comparative evaluation of cytotoxic activity. Research Journal of Pharmacy and Technology. 2020; 13(2): 659-663. Doi: 10.5958/0974-360X.2020.00126.2
11. Sharma S, Yadav P, Singh B, et al. Antimicrobial activity of the soxhlet extraction of Plumbago zeylanica leaf extracts in-vitro conditions. Research Journal of Pharmacy and Technology. 2012; 5(10): 1297-1300.
12. Ghasemi S, Dabirian S, Kariminejad F, et al. Process optimization for green synthesis of silver nanoparticles using Rubus discolor leaves extract and its biological activities against multi-drug-resistant bacteria and cancer cells. Sci Rep; 2024; 14: 4130. Doi: 10.1038/s41598-024-54702-9.
13. Argade PA, Bhutkar MA, Magdum CS. Albizzia lebbeck extract mediated synthesis of zinc oxide nanoparticles and study of its in-vitro anti-diabetic and anti-oxidant activity. Asian Journal of Pharmacy and Technology. 2019; 9: 93.Doi:10.5958/2231-5691.2019.00001.7.
14. Chirakkara SP, George J, Abraham A. Mice ovarian microbiome investigation divulges prospective fount of anticancer and antimicrobial metabolites. Research Journal of Pharmacy and Technology. 2023; 16(10): 4847-4.Doi:10.52711/0974-360X.2023.00786.
15. Liaqat N, Jahan N, Khalil-ur-Rahman, et al. Green synthesized silver nanoparticles: Optimization, characterization, antimicrobial activity, and cytotoxicity study by hemolysis assay. Front Chem 2022; 10: 952006. Doi: 10.3389/fchem.2022.952006.
16. Fahri AN, Heryanto H, Tahir D. Mie Scattering theory for identifying surface plasmon resonances (SPR) by the finite-size model: Theoretical study of gold-silver core–shell nanospheres. Arab J Sci Eng 2023; 48: 789–801. Doi: 10.1007/s13369-022-06968-2.
17. Anandalakshmi K, Venugobal J, Ramasamy V. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Applied Nanoscience. 2016; 6: 399–408. Doi:10.1007/s13204-015-0449-z.
18. D.P. Pradeep, Oommen P. Saj. Phytochemical and antimicrobial studies of leaf and bark extracts of Holigarna arnottiana Hook f. Journal of Pure and Applied Microbiology 2010; 139–148.
19. Ail PD, Jyothi D, Sultana R, et al. Synthesis and optimization of silver nanoparticles using leaf extract of Tamarindus indica by box-behnken design for enhanced antimicrobial activity. Research Journal of Pharmacy and Technology. 2023; 16(10): 4583-0. Doi: 10.52711/0974-360X.2023.00746.
20. Vanlalveni C, Lallianrawna S, Biswas A, et al. Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature. RSC Advances. 2021; 11: 2804–2837. Doi:10.1039/D0RA09941D
21. Azarbani F, Shiravand S. Green synthesis of silver nanoparticles by Ferulago macrocarpa flowers extract and their antibacterial, antifungal and toxic effects. Green Chemistry Letters and Reviews 2020; 13: 41–49. Doi:10.1080/17518253.2020.1726504.
22. Asha S, Thirunavukkarasu P, Rajeshkumar S. Green synthesis of silver nanoparticles using Mirabilis jalapa aqueous extract and their antibacterial activity against respective microorganisms. Res J Pharm Technol. 2017; 10: 811–817. Doi: 10.5958/0974-360X.2017.00153.6.
23. Maheshwari S, Tiwari RK, Singh L. Green expertise: Synthesis of silver nanoparticles for wound healing application an overview. Research Journal of Pharmacy and Technology. 2021; 14: 1149–1154. Doi: 10.5958/0974-360X.2021.00206.7.
24. Abdulazeem L, Al Jassani MJ, Al-Sheakh MA. Free radical scavenging and antioxidant activity of silver nanoparticles synthesized from Cuminum cyminum (Cumin) seed extract. Res J Pharm Technol. 2021; 14: 4349–4354. Doi: 10.52711/0974-360X.2021.00755
25. Rathi MA. Green synthesis and characterization of silver nano particles from Alpenia purpurata. Pharmacologyonline. 2018; 1: 33–43.
26. Asha Monica A, Senthilkumar SR. Green synthesis and characterization of silver nanoparticles from Ocimum basilicum and their antimicrobial antioxidant and anticancer activity. Res J Pharm Technol. 2020; 13: 5711–5715. Doi: 10.5958/0974-360X.2020.00994.4