Author(s):
Erna Prawita Setyowati, Diah Tri Utami, Sari Haryanti, Rony Abdi Syahputra, Aminah Dalimunthe, Yahdiana Harahap, Bonglee Kim
Email(s):
erna_prawita@ugm.ac.id
DOI:
10.52711/0974-360X.2026.00095 
Address:
Erna Prawita Setyowati1*, Diah Tri Utami2, Sari Haryanti3, Rony Abdi Syahputra4, Aminah Dalimunthe4, Yahdiana Harahap5, Bonglee Kim6
1Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
2Department of Pharmacy, Faculty of Medicine and Health Sciences, Universitas Jambi, Indonesia.
3Medicinal Plant and Traditional Medicine Research and Development Centre, Ministry of Health, Indonesia. 4Faculty of Pharmacy, University of North Sumatra, Indonesia.
5Faculty of Pharmacy, University of Indonesia, Indonesia.
6Kyung Hee University, Republic of Korea.
*Corresponding Author
Published In:
Volume - 19,
Issue - 2,
Year - 2026
ABSTRACT:
Research on the antioxidant and cytotoxic activity of Litsea cubeba (LC) extracts on B1610, 3T3, and Vero cell lines has been conducted. This study also analyzed the compound content of LC peel, fruit, and leaf extracts using the HRMS method. This research is expected to determine the most potent and safe extract for use as a cosmetic agent, specifically a whitening agent from the Litsea cubeba (Lour.) Pers plant. The results showed that using the DPPH and BCB methods, the LC leaf extract provided the most substantial IC50 results. Using the ABTS method, the most substantial results were obtained from the LC fruit extract. Compared with other LC cell parts, the LC leaf extract had the highest potential cytotoxic activity with an IC50 value of 35.81±1.85µg/ml. The SI values of 8.48 and 12.73, respectively, indicate that the extract is more selective against B16F10 melanoma cells than normal Vero and 3T3 cells. HRMS results showed that the extracts from the three LC parts contained approximately 600-650 compounds. There were similarities in the compound content, namely 9-Oxo-10(E), 12(E)-octadecadienoate, and (-)-Caryophyllene oxide, which were in the range of 0.5-1.4%. The LC bark extract gave a selectivity index <1, possibly due to the high content of the toxic compound gaxsolidine (7.78%).
Cite this article:
Erna Prawita Setyowati, Diah Tri Utami, Sari Haryanti, Rony Abdi Syahputra, Aminah Dalimunthe, Yahdiana Harahap, Bonglee Kim. Analysis of Compound content in Bark, Fruit and Leaves of Listea cubeba (LOUR.) Pers using HRMS: Antioxidant and Cytotoxic activity against B16F10, 3T3 and Vero Cells. Research Journal of Pharmacy and Technology. 2026;19(2):651-6. doi: 10.52711/0974-360X.2026.00095
Cite(Electronic):
Erna Prawita Setyowati, Diah Tri Utami, Sari Haryanti, Rony Abdi Syahputra, Aminah Dalimunthe, Yahdiana Harahap, Bonglee Kim. Analysis of Compound content in Bark, Fruit and Leaves of Listea cubeba (LOUR.) Pers using HRMS: Antioxidant and Cytotoxic activity against B16F10, 3T3 and Vero Cells. Research Journal of Pharmacy and Technology. 2026;19(2):651-6. doi: 10.52711/0974-360X.2026.00095 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-2-24
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