Author(s):
Sameea Ahmed Khan, Rajesh Sharma
Email(s):
sameeak63@gmail.com
DOI:
10.52711/0974-360X.2026.00654 
Address:
Sameea Ahmed Khan*, Rajesh Sharma
School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road (Ring Road), Indore, M.P., India, 452001.
*Corresponding Author
Published In:
Volume - 19,
Issue - 10,
Year - 2026
ABSTRACT:
In this work, we present a summary of the synthesis and characterization of Super-Paramagnetic Iron Oxide Nanoparticles (SPIONs) and their behavior in vitro and in vivo. Therefore, we have used various characterization techniques to understand the surface morphology as well as magnetic properties. These nanoparticles were coated with Ascorbic acid (AA) and the synthesized formulation was used in the treatment of iron-deficiency anemia (IDA). In vitro dissolution studies indicated the better release of AA as compared to the marketed formulation. Iron content assay was performed through UV-visible spectroscopy to determine the percentage of iron in the formulation. Furthermore, stability studies were also conducted to clearly understand the application aspect of the formulation. Finally, in vivo studies were performed and various biochemical parameters were closely observed and statistically analyzed. Histopathological studies were also performed on the isolated spleen tissues of the Wistar rats. The results presented that the iron uptake increased significantly in Wistar rats, due to the nano-sized particles and the coating of AA. It is shown that AA significantly improved the absorption of iron in the body, thereby aiding in reduced dose and better therapeutic efficacy.
Cite this article:
Sameea Ahmed Khan, Rajesh Sharma. Super-Paramagnetic Iron Oxide Nanoparticles (SPIONs): From Synthesis to In vivo Studies: A study for the treatment of Iron-Deficiency Anemia. Research Journal Pharmacy and Technology. 2026;19(10):4697-2. doi: 10.52711/0974-360X.2026.00654
Cite(Electronic):
Sameea Ahmed Khan, Rajesh Sharma. Super-Paramagnetic Iron Oxide Nanoparticles (SPIONs): From Synthesis to In vivo Studies: A study for the treatment of Iron-Deficiency Anemia. Research Journal Pharmacy and Technology. 2026;19(10):4697-2. doi: 10.52711/0974-360X.2026.00654 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-10-30
REFERENCES:
1. Kujovich JL. Evaluation of anemia. Obstetrics and Gynecology Clinics. 2016 Jun 1; 43(2): 247-64.
2. Weiss G, Ganz T, Goodnough LT. Anemia of inflammation. Blood, The Journal of the American Society of Hematology. 2019 Jan 3; 133(1): 40-50.
3. Nemeth E, Ganz T. Anemia of inflammation. Hematology/Oncology Clinics. 2014 Aug 1; 28(4): 671-81.
4. Goodnough LT, Schrier SL. Evaluation and management of anemia in the elderly. American Journal of Hematology. 2014 Jan;89(1):88-96.
5. Lanier JB, Park JJ, Callahan RC. Anemia in older adults. American Family Physician. 2018 Oct 1; 98(7): 437-42.
6. Gallagher PG. Anemia in the pediatric patient. Blood, The Journal of the American Society of Hematology. 2022 Aug 11; 140(6): 571-93.
7. Short MW, Domagalski JE. Iron deficiency anemia: evaluation and management. American Family Physician. 2013 Jan 15; 87(2): 98-104.
8. Auerbach M, Adamson JW. How we diagnose and treat iron deficiency anemia. American journal of hematology. 2016 Jan; 91(1): 31-8.
9. Gupta PM, Perrine CG, Mei Z, Scanlon KS. Iron, anemia, and iron deficiency anemia among young children in the United States. Nutrients. 2016 May 30; 8(6): 330.
10. Wang M. Iron deficiency and other types of anemia in infants and children. American Family Physician. 2016 Feb 15; 93(4): 270-8.
11. Camaschella C. Iron-deficiency anemia. New England Journal of Medicine. 2015 May 7;372(19):1832-43.
12. Jimenez K, Kulnigg-Dabsch S, Gasche C. Management of iron deficiency anemia. Gastroenterology and Hepatology. 2015 Apr; 11(4): 241.
13. Tvedten H. Classification and laboratory evaluation of anemia. Schalm's Veterinary Hematology. 2022 Apr 22: 198-208.
14. Short MW, Domagalski JE. Iron deficiency anemia: evaluation and management. American Family Physician. 2013 Jan 15; 87(2): 98-104.
15. Baviera LB. Anemia ferropénica. Pediatria Val Serrería II Val. 2016 Jun: 297-308.
16. Koury MJ. Abnormal erythropoiesis and the pathophysiology of chronic anemia. Blood Reviews. 2014 Mar 1; 28(2): 49-66.
17. Nairz M, Theurl I, Wolf D, Weiss G. Iron deficiency or anemia of inflammation? Differential diagnosis and mechanisms of anemia of inflammation. Wiener Medizinische Wochenschrift. 2016 Oct;166(13):411-23.
18. D'angelo G. Role of hepcidin in the pathophysiology and diagnosis of anemia. Blood research. 2013 Mar; 48(1): 10.
19. Gaspar BL, Sharma P, Das R. Anemia in malignancies: pathogenetic and diagnostic considerations. Hematology. 2015; 20(1): 18-25.
20. Damian MT, Vulturar R, Login CC, Damian L, Chis A, Bojan A. Anemia in sports: a narrative review. Life. 2021 Sep 20;11(9):987.
21. DeLoughery TG. Iron deficiency anemia. Medical Clinics. 2017; 101(2): 319-32.
22. Koury MJ. Abnormal erythropoiesis and the pathophysiology of chronic anemia. Blood Reviews. 2014; 28(2): 49-66.
23. Jamwal M, Sharma P, Das R. Laboratory approach to hemolytic anemia. The Indian Journal of Pediatrics. 2020; 87: 66-74.
24. Pagani A, Nai A, Silvestri L, Camaschella C. Hepcidin and anemia: a tight relationship. Frontiers in Physiology. 2019 Oct 9; 10: 494963.
25. Pasricha SR, Drakesmith H, Black J, Hipgrave D, Biggs BA. Control of iron deficiency anemia in low-and middle-income countries. Blood, the Journal of the American Society of Hematology. 2013 Apr 4; 121(14): 2607-17.
26. Sorice A, Guerriero E, Capone F, Colonna G, Castello G, Costantini S. Ascorbic acid: its role in immune system and chronic inflammation diseases. Mini Reviews in Medicinal Chemistry. 2014 Apr 1; 14(5): 444-52.
27. Gęgotek A, Skrzydlewska E. Ascorbic acid as antioxidant. Vitamins and Hormones. 2023 Jan 1; 121: 247-70.
28. Bilska K, Wojciechowska N, Alipour S, Kalemba EM. Ascorbic acid—The little-known antioxidant in woody plants. Antioxidants. 2019 Dec 14;8(12):645.
29. Talluri S, Malla RR. Superparamagnetic iron oxide nanoparticles (SPIONs) for diagnosis and treatment of breast, ovarian and cervical cancers. Current Drug Metabolism. 2019 Oct 1; 20(12): 942-5.