Author(s):
Mariam Ulfah, Like Efriani, Tonasih, Ismanurrahman Hadi, Ade Irawan, Teguh Adiyas Putra, I Made Bayu Anggriawan
Email(s):
mariamulfah24@gmail.com
DOI:
10.52711/0974-360X.2026.00053 
Address:
Mariam Ulfah1*, Like Efriani1, Tonasih2, Ismanurrahman Hadi1, Ade Irawan1, Teguh Adiyas Putra1, I Made Bayu Anggriawan3
1Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Ahmad Dahlan Cirebon, Indonesia
2Department of Midwifery, Faculty of Health Sciences, Universitas Muhammadiyah Ahmad Dahlan Cirebon, Indonesia.
3College of Medicine, Veterinary and Life Science, School of Medicine, Densitiry and Nursing, Clinical Pharmacology Wolfson Medical School University Glasglow, London.
*Corresponding Author
Published In:
Volume - 19,
Issue - 1,
Year - 2026
ABSTRACT:
The world's biggest cause of death for women is breast cancer. High glucose levels in diabetes mellitus can support the development of cancer. The use of traditional medicine is increasingly urgent due to its potential therapeutic benefits for the treatment of diabetes and cancer. White lotus (Nymphaea alba) and mangrove (Rhizophora mucronata) are aquatic plants that have many pharmacological activities, including antidiabetic, anticancer, and antioxidant. In this study, a combination of the two extracts was carried out to obtain better anticancer and antidiabetic activity. The combination of the extracts was mangrove: lotus = 25%: 75% (combination extract A), mangrove: lotus = 50%: 50% (combination extract B), and mangrove: lotus = 75%: 25% (combination extract C). The process involved macerating mangrove and lotus leaves with ethanol solvent, then combining the resulting extracts to create extracts A, B, and C. The biological activity of these three extracts, as well as the lotus and mangrove extracts, was subsequently examined in vitro test. Specifically, the resazurin method was used to assess the extracts' anticancer on MCF-7 cancer cells and antidiabetic effects toward the alpha glucosidase enzyme, respectively. The results of this study are that mangrove has the best antidiabetic and anticancer activity with IC50 values of 19.90 ± 0.62 and 50.11 µg/mL, respectively. The best combination of extracts is combination C with a ratio of mangrove and lotus extracts of 75%: 25% with IC50 antidiabetic and anticancer values of 1.68 ± 0.70 (highly active) and 76.26 µg/mL (active), respectively. The conclusion of this study is that the combination of white lotus leaf and mangrove extracts has great potential as an antidiabetic and anticancer agent.
Cite this article:
Mariam Ulfah, Like Efriani, Tonasih, Ismanurrahman Hadi, Ade Irawan, Teguh Adiyas Putra, I Made Bayu Anggriawan. Antibreast Cancer and Antidiabetic Activity of Combination of Nymphae alba and Rhizophora mucronata Ethanolic Leaf Extract. Research Journal of Pharmacy and Technology. 2026;19(1):364-1. doi: 10.52711/0974-360X.2026.00053
Cite(Electronic):
Mariam Ulfah, Like Efriani, Tonasih, Ismanurrahman Hadi, Ade Irawan, Teguh Adiyas Putra, I Made Bayu Anggriawan. Antibreast Cancer and Antidiabetic Activity of Combination of Nymphae alba and Rhizophora mucronata Ethanolic Leaf Extract. Research Journal of Pharmacy and Technology. 2026;19(1):364-1. doi: 10.52711/0974-360X.2026.00053 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-1-53
REFERENCES:
1. World Health Organization. Global Report on Diabetes. Isbn. 2016; 978: 88. doi: ISBN 978 92 4 156525 7
2. Webber S. International Diabetes Federation. Vol 102.; 2013. doi:10.1016/j.diabres.2013.10.013
3. Release P. World Cancer Day: Breast cancer overtakes lung cancer in terms of number of new cancer cases worldwide. IARC showcases key research projects to address breast cancer – IARC. 2021; (February):2020-2022.
4. Zhao XB, Ren GS. Diabetes mellitus and prognosis in women with breast cancer: A systematic review and meta-analysis. Med (United States). 2016; 95(49): e5602. doi:10.1097/MD.0000000000005602
5. Li W, Zhang X, Sang H, et al. Effects of hyperglycemia on the progression of tumor diseases. J Exp Clin Cancer Res. 2019; 38(1): 1-7. doi:10.1186/s13046-019-1309-6
6. Dattatraya SK, Dattatray SK, Sunil KG, Suryakant MS. Formulation and evaluation of herbal antidiabetic tablet. Asian J Res Pharm Sci. 2020; 10(3): 145. doi:10.5958/2231-5659.2020.00027.2
7. Babu G, Bhaumik A, Krishnamachary K, Kalyani P. Synthetic Novel Flavanoid derivatives act as potential Antidiabetic agent against Streptozocin induced in diabetic Rats. Asian J Res Chem. 2018; 11(5): 799. doi:10.5958/0974-4150.2018.00141.4
8. Bose A, Ray S, Sahoo M. Evaluation of analgesic and antioxidant potential of ethanolic extract of <i>Nymphaea alba</i> rhizome. Oxid Antioxid Med Sci. 2012; 1(3): 217. doi:10.5455/oams.140912.or.017
9. Yildirim AB, Karakas FP, Turker AU. In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey. Asian Pac J Trop Med. 2013; 6(8): 616-624. doi:10.1016/S1995-7645(13)60106-6
10. Irawan A, Ulfah M, Putra TA, et al. Aktivitas Antijamur Ekstrak Aseton Daun Teratai Putih (Nymphae alba) Terhadap Jamur Candida albicans. 2023; 6(2):794-800.
11. Singh G, Agnihotri S, Verma SK. In vivo and in vitro Antidiabetic Characterization of Nymphaea alba leaves. Int J Pharm Sci Drug Res. Published online September 30, 2020: 565-571. doi:10.25004/ijpsdr.2020.120520
12. Ulfah M, Irawan A, Putra TA. Jurnal Farmasi Sains Dan Praktis Aktivitas Antibakteri Ekstrak Aseton Dan Etil Asetat Daun, Batang Dan Rimpang Teratai Putih (Nymphaeae Alba) Terhadap Bakteri Staphylococcus Aureus Dan Escherichia Coli Antibacterial Activity of Acetone and Ethyl Acetate Leaves, Stems and Rhizomes Extract of White Water Lily (Nymphaeae Alba) Against Staphylococcus Aureus and Escherichia Coli. Vol 5.; 2019. http://journal.ummgl.ac.id/index.php/pharmacy
13. Ulfah M, Irawan A, Putra TA. Tunas Medika Jurnal Kedokteran & Kesehatan Aktivitas Antibakteri Ekstrak Aseton Teratai Putih (Nymphaeae Alba) Terhadap Bakteri Streptococcus Pyogenes. http://jurnal.unswagati.ac.id/index.php/tumed
14. Ulfah M, Putra TA, Irawan A. Antiobesity activity of White lotus (Nymphaea alba) acetone extract in wistar strains rats (Rattus norvegicus L ). 2022; IX(2): 14-20.
15. Gosavi N, D. Chaudhari D, E. Jagdale D, R. Jaiswal N. Formulation and Evaluation of Polyherbal Lotus Oil. Res J Top Cosmet Sci. 2023; 14(1): 29-44. doi:10.52711/2321-5844.2023.00006
16. Kasitowati RD, Yamindago A, Safitri M. Potensi Antioksidan Dan Skrining Fitokimia Ekstrak Daun Mangrove Rhizophora mucronata, Pilang Probolinggo. JFMR-Journal Fish Mar Res. 2017; 1(2): 72-77. doi:10.21776/ub.jfmr.2017.001.02.4
17. Rahmawati R, Sanjaya YA, Pratiwi YS. Potency of Mangrove Leaves (Rhizophora mucronata) Containing Bioactive Compounds as Source of Antioxidant : A Review. 2023; 04(2): 1-7.
18. Saragih G, Tamrin, Marpongahtun, Nasution DY, Abdillah. Phytochemical screening and toxicity of ethanolic extract of mangrove (Rhizophora mucronata) leaves from Langsa, Aceh Timur. Rasayan J Chem. 2020; 13(1): 476-480. doi:10.31788/RJC.2020.1315524
19. Chandrika P, Prasad KRS, Phani.R. S. Ch. Phytochemical screening and evaluation of anti-bacterial activity and various anti-oxidant studies of root extracts of mangrove plant dalbergia spinosa. Res J Pharm Technol. 2020; 13(6): 2551-2555. doi:10.5958/0974-360X.2020.00454.0
20. Youssef AMM, Maaty DAM, Al-Saraireh YM. Phytochemistry and Anticancer Effects of Mangrove (Rhizophora mucronata Lam.) Leaves and Stems Extract against Different Cancer Cell Lines. Pharmaceuticals. 2023; 16(1). doi:10.3390/ph16010004
21. Efriani L, Hadi I, Irawan A, Ulfah M, Adiyas Putra T. UJI Aktivitas Inhibisi Enzim a-Glukosidase Terhadap Ekstrak Aseton, Etanol, DAN Methanol Daun Mangrove (Rhizopora Mucronata) Sebagai Antidiabetes. Med Sains J Ilm Kefarmasian. 2023; 8(4): 1371-1378. doi:10.37874/ms.v8i4.651
22. Mesta SC, Onkarappa R, Meghana SH, Manu HM, Kavana S, Talib M. Phosphate solubilizing endophytic actinomycetes from mangrove plants Rhizophora mucronata and Sonneratia caseolaris and its effect on seedling vigour. Res J Pharm Technol. 2018; 11(3): 1172-1178. doi:10.5958/0974-360X.2018.00219.6
23. Shelar MK, Patil MJ, Bhujbal SS. Phytochemical and pharmacognostical evaluation of milky mangrove excoecaria agallocha linn. Res J Pharm Technol. 2019; 12(3): 1289-1293. doi:10.5958/0974-360X.2019.00216.6
24. Kesuma RA, Kustanti A, Hilmanto R. Diameter increment growth of bakau kurap (Rhizophora mucronata) in Lampung Mangrove center. J Sylva Lestari. 2016; 4(3): 97-106.
25. Ramena GO, V Wuisang CE, P Siregar FO. Pengaruh Aktivitas Masyarakat Terhadap Ekosistem Mangrove Di Kecamatan Mananggu. J Spasial. 2020; 7(3): 343-351.
26. Usman, Muh Amir M, Erika F, Nurdin M, Kuncoro H. Antidiabetic activity of leaf extract from three types of mangrove originating from sambera coastal region Indonesia. Res J Pharm Technol. 2019; 12(4): 1707-1712. doi:10.5958/0974-360X.2019.00284.1
27. Lalitha P, Sachithanandam V, Swarnakumar NS, Sridhar R. Review on Anti-inflammatory Properties of Mangrove plants. Asian J Pharm Res. 2019; 9(4): 273. doi:10.5958/2231-5691.2019.00045.5
28. Hari R, Vadivu R, Radha R. Effect of leaf extract from mangrove species rhizophora mucronata poir on homocysteine induced coagulation factors. Res J Pharm Technol. 2019; 12(10): 4807-4811. doi:10.5958/0974-360X.2019.00831.X
29. Rajani Kumari P, Prasanna Kumari YASJ, Vijaya Kumari C. In-vitro pharmacological evaluation of leaf extracts of a medicinal mangrove plant bruguiera gymnorhiza L. Res J Pharm Technol. 2020; 13(4): 1865-1870. doi:10.5958/0974-360X.2020.00336.4
30. Ranjith Babu K, Kumar N, Solepure AB, Shaikh R. International Journal of Biomedical Research Comparison of hematological parameters in primary hypertensives and normotensives of Sangareddy. Int J Biomed Res. 2015; 6(05): 5. doi:10.7439/ijbr
31. Dewi RT, Tachibana S, Darmawan A. Effect on α-glucosidase inhibition and antioxidant activities of butyrolactone derivatives from Aspergillus terreus MC751. Med Chem Res. 2014; 23(1): 454-460. doi:10.1007/s00044-013-0659-4
32. Petiti J, Revel L, Divieto C. Standard Operating Procedure to Optimize Resazurin-Based Viability Assays. Biosensors. 2024; 14(4). doi:10.3390/bios14040156
33. Kumaradewi DAP, Subaidah WA, Andayani Y, Al-Mokaram A. Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method. J Penelit Pendidik IPA. 2021; 7(2): 275. doi:10.29303/jppipa.v7i2.675
34. Cudalbeanu M, Ghinea IO, Furdui B, et al. Exploring New Antioxidant and Mineral Compounds from Nymphaea alba Wild-Grown in Danube Delta Biosphere.:1-16. doi:10.3390/molecules23061247
35. Culas S, Marapana RAUJ, Palangasinghe IR, Liyanage AC. Development of Liquid-Based Tea and Its Antidiabetic Effect. J Chem. 2021; 2021. doi:10.1155/2021/8863936
36. Vieira-da-Silva B, Castanho MARB. Resazurin Reduction-Based Assays Revisited: Guidelines for Accurate Reporting of Relative Differences on Metabolic Status. Molecules. 2023; 28(5). doi:10.3390/molecules28052283
37. Atjanasuppat K, Wongkham W, Meepowpan P, et al. In vitro screening for anthelmintic and antitumour activity of ethnomedicinal plants from Thailand. J Ethnopharmacol. 2009; 123(3): 475-482. doi:10.1016/j.jep.2009.03.010
38. Kumaradewi DAP, Subaidah WA, Andayani Y, Al-Mokaram A. Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method. J Penelit Pendidik IPA. 2021; 7(2): 275. doi:10.29303/jppipa.v7i2.675
39. Olsson AR, Lindgren H, Pero RW, Leanderson T. Mechanism of action for N-substituted benzamide-induced apoptosis. Br J Cancer. 2002;86:971-978. doi:10.1038/sj/bjc/6600136
40. Ali A. Development of antidiabetic drugs from benzamide derivatives as glucokinase activator: A computational approach. Saudi J Biol Sci. 2022; 29(5): 3313-3325. doi:10.1016/j.sjbs.2022.01.058
41. Thakal S, Singh A, Singh V. In vitro and in silico evaluation of N-(alkyl/aryl)-2-chloro-4-nitro-5- [(4-nitrophenyl)sulfamoyl]benzamide derivatives for antidiabetic potential using docking and molecular dynamic simulations. J Biomol Struct Dyn. 2022; 40(9): 4140-4163. doi:10.1080/07391102.2020.1854116
42. Bawadi HA, Bansode RR, Trappey A, Truax RE, Losso JN. Inhibition of Caco-2 colon, MCF-7 and Hs578T breast, and DU 145 prostatic cancer cell proliferation by water-soluble black bean condensed tannins. Cancer Lett. 2005; 218(2): 153-162. doi:10.1016/j.canlet.2004.06.021
43. Huang Q, Chai WM, Ma ZY, et al. Inhibition of α-glucosidase activity and non-enzymatic glycation by tannic acid: Inhibitory activity and molecular mechanism. Int J Biol Macromol. 2019; 141: 358-368. doi:10.1016/j.ijbiomac.2019.09.010