Author(s):
Nemala Appala Raju, Anitha Nandagopal, Anupama Koneru
Email(s):
anitha@sucp.ac.in
DOI:
10.52711/0974-360X.2026.00043 
Address:
Nemala Appala Raju1, Anitha Nandagopal2*, Anupama Koneru3
1Department of Pharmcognosy, Sultan-ul-Uloom College of Pharmacy, Road No.3, Banjara Hills, Hyderabad-500034, Telangana, India.
2Department of Pharmacology, Sultan-ul-Uloom College of Pharmacy, Road No.3, Banjara Hills, Hyderabad-500034, Telangana, India.
3Department of Pharmacology, Sultan-ul-Uloom College of Pharmacy, Road No.3, Banjara Hills, Hyderabad-500034, Telangana, India.
*Corresponding Author
Published In:
Volume - 19,
Issue - 1,
Year - 2026
ABSTRACT:
Cancer continues to pose a significant global health challenge, necessitating the development of innovative treatments. Plants containing bioactive compounds present a significant opportunity for cancer research. Averrhoa bilimbi, a relatively obscure fruit, possesses multiple traditional medicinal applications, such as the treatment of inflammation, respiratory ailments, and dermatological conditions. This research assessed the cytotoxic effects of n-hexane, ethyl acetate, and methanolic extracts from A. bilimbi leaves on many types of cancer cells, including Vero, pancreatic, lung, oesophageal squamous carcinoma (KYSE 150), and rhabdomyosarcoma (RD), employing the MTT assay. Apoptosis was evaluated utilizing Caspase 3/7 kits. The ethyl acetate extract demonstrated significant anticancer activity against RD cells (IC50: 19.09 ± 12.86), and both ethyl acetate and methanolic extracts were effective against KYSE cells. A. bilimbi leaf extracts may promote cancer cell death through apoptosis, suggesting chemotherapeutic potential.
Cite this article:
Nemala Appala Raju, Anitha Nandagopal, Anupama Koneru. Cytotoxic Effect of Averrhoa bilimbi Extracts Through MTT and Caspase 3/7 Assays in Vero, KYSE 150, RD, AsPC-1, and A549 Cell lines. Research Journal of Pharmacy and Technology. 2026;19(1):301-7. doi: 10.52711/0974-360X.2026.00043
Cite(Electronic):
Nemala Appala Raju, Anitha Nandagopal, Anupama Koneru. Cytotoxic Effect of Averrhoa bilimbi Extracts Through MTT and Caspase 3/7 Assays in Vero, KYSE 150, RD, AsPC-1, and A549 Cell lines. Research Journal of Pharmacy and Technology. 2026;19(1):301-7. doi: 10.52711/0974-360X.2026.00043 Available on: https://rjptonline.org/AbstractView.aspx?PID=2026-19-1-43
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