Author(s):
Prathibha Bharathi Mare, Subash Chandra Bose Penjuri, Siva Prasad Sagili, Ceema Mathew, Satish Kumar Meruva
Email(s):
mprprathibha@gmail.com , prathibha8086@grcp.ac.in
DOI:
10.52711/0974-360X.2025.00636 
Address:
Prathibha Bharathi Mare1*, Subash Chandra Bose Penjuri2, Siva Prasad Sagili3, Ceema Mathew4, Satish Kumar Meruva5
1,4Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad, Telangana, 500090, India.
3,2,5MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana, 502001, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 9,
Year - 2025
ABSTRACT:
The present study was designed to study the in vitro antioxidant activity and antibacterial activities of Atylosia rugosa. The antioxidant activities of the extracts were examined using different biochemical assays namely diphenlpicrylhydrazyl (DPPH), hydrogen peroxide radical scavenging activity and the antibacterial activity of the extracts was also evaluated and MIC values were calculated by broth dilution method. The assays, revealed that the ethyl acetate extract exhibited stronger antioxidant activity than that of ethanolic extract, the extracts also prevented the growth of both Gram positive and Gram negative bacteria, the MIC values of ethanolic extract has higher antibacterial than those of the ethyl acetate. The antioxidant and antibacterial activity of the extracts was found to be positively associated with the total phenolic and favonoid content of the extracts. A positive, significant linear relationship between antioxidant activity and TPC and TFC content showed that phenolic compounds and flavonoids were the dominant and possessing antioxidant and antibacterial properties of Atylosia rugosa.
Cite this article:
Prathibha Bharathi Mare, Subash Chandra Bose Penjuri, Siva Prasad Sagili, Ceema Mathew, Satish Kumar Meruva. Evaluation of Antioxidant and Antibacterial activities of Atylosia rugosa. Research Journal of Pharmacy and Technology. 2025;18(9):4435-1. doi: 10.52711/0974-360X.2025.00636
Cite(Electronic):
Prathibha Bharathi Mare, Subash Chandra Bose Penjuri, Siva Prasad Sagili, Ceema Mathew, Satish Kumar Meruva. Evaluation of Antioxidant and Antibacterial activities of Atylosia rugosa. Research Journal of Pharmacy and Technology. 2025;18(9):4435-1. doi: 10.52711/0974-360X.2025.00636 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-9-56
REFERENCES:
1. Khalid H, Abdalla WE, Abdelgadir H, Opatz T, Efferth T. Gems from traditional north-African medicine: medicinal and aromatic plants from Sudan. Nat. Prod. Bioprospect. 2012; 2(1): 92–103. doi.org/10.1007/s13659-012-0015-2.
2. Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis extracts Torilis leptophylla L. BMC Complement. Altern. Med. 2012; 12: 221. doi: 10.1186/1472-6882-12-221.
3. The world health report: reducing risks, promoting healthy life. Geneva, World Health Organization. 2002. DOI: 10.1080/1357628031000116808.
4. Barton D, Nakanishi K, Meth-Cohn O. Comprehensive natural products chemistry, Elsevier Science Ltd, London 1999.4(1). DOI: 10.1186/1472-6882-12-221.
5. Rizvi SAA, Einstein GP, Tulp OL, Sainvil F, Branly R. Introduction to Traditional Medicine and Their Role in Prevention and Treatment of Emerging and Re-Emerging Diseases. Biomolecules. 2022; 12(10): 1442. doi: 10.3390/biom12101442.
6. Halliwell B. Oxidative stress and neurodegeneration; where are we now?. J. Neuro chem, 2006; 97: 1634–1658. doi: 10.1111/j.1471-4159.2006.03907.
7. Halliwell B, Oxidative stress and cancer: Have we moved forward?, Biochem. J, 2007; 401: 1–11. doi: 10.1042/BJ20061131.
8. Ferguson L.R. Chronic inflammation and mutagenesis. Mutat. Res. Fund. Mol. M. 2007; 690: 3–11. doi: 10.1016/j.mrfmmm.2010.03.007.
9. Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas E.N, Lakshminarasaiah U, etal. Antioxidants and Human Diseases. Clin. Chim. Acta. 2014; 436: 332–347. doi.org/10.1016/j.cca.2014.06.004.
10. Sampaio TL, Menezes RR, da Costa MF, Meneses GC, Arrieta M.C, Filho AJC. In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan. Phyto Med. 2016; 23(1): 1843–1852. doi: 10.1007/s13659-018-0155.
11. Youssef FS, Ashour ML, Sobeh M, El-Beshbishy HA, Singab AN, M. Wink. Phyto Med. 2016; 23: 1484–1493. doi: 10.3390/molecules25061341.
12. Suchal K, Malik S, Gamad N, Malhotra RK, Goyal SN, Bhatia J. Kampeferol Protects Against Oxidative Stress and Apoptotic Damage in Experimental Model of Isoproterenol-Induced Cardiac Toxicity in Rat. Phyto Med. 2016; 23(1): 1401–1408. doi: 10.1016/j.phymed.2016.07.015.
13. Subedi L, Gaire BP, Do MH, Lee TH, Kim SY. Anti-neuroinflammatory and Neuroprotective Effects of the Lindera Neesiana Fruit in Vitro. Phyto Med. 2016; 23(1): 872–881. doi: 10.1016/j.phymed.2016.05.002.
14. Simeonova R, Vitcheva V, Krasteva I, Zdraveva P, Konstantinov S, Ionkova I . Hepatoprotective effects of saponarin, isolated from Gypsophila trichotoma Wend. on cocaine-induced oxidative stress in rats. Phyto Med. 2016; 23(1): 483–490. 3. doi: 10.1016/j.phymed.2016.05.002.
15. Cragg GM, Newman DJ. Natural product drug discovery in the next millennium. Pharm Biol. 2001; 39 (1): 8- doi: 10.1076/phbi.39.s1.8.0009.
16. Rates SM. Plants as source of drugs. Toxicon. 2001; 39: 603-613. doi: 10.1016/s0041-0101(00)00154-9.
17. Devasagayam TP. Introduction to serial reviews: Recent Advances in Indian Herbal Drug Research. J Clin Biochem Nutr. 2007; 40: 73. DOI: 10.3164/jcbn.40.73.
18. Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TP. Indian Herbs and Herbal Drugs used for the treatment of Diabetes. J Clin Biochem Nutr. 2007; 40(1): 163 -173. doi: 10.5455/jice.20141030015241.
19. Heritage JM, Zali FH, Gascoyne-Binzi D, Hawkey PM. Evolution and spread of SHV extended-spectrum beta-lactamases in gram-negative bacteria. J Antimicrob Chemother. 1999; 449(2): 309-318. doi: 10.1093/jac/44.3.309.
20. Andersson DI, Hughes D. Antibiotic resistance and its cost: is it possible to reverse resistance?. Nature Rev Microbiol. 2010; 8: 260-271. doi: 10.1093/jac/44.3.309.
21. Panigrahy B, Grumbles LC, Millar D, Naqi S.A, Hall CF. Antibiotic-induced immunosuppression and Levamisole-induced immunopotentiation in Turkey. Avian Diseases. 1979; 23: 401-408. doi: 10.4314/ovj.v9i4.13.
22. Tsuruga M, Nakajima H, Magae J. Immunosuppressive activity of 4-O-Methylascochlorin, The Journal of Antibiotics. 2007: 60: 20-26. doi: 10.1038/ja.2007.3.
23. Peschel W, Sanchez-Rabaneda F, Dieckmann W, Plescher A, Gartzia, I. An industrial approach in the search of natural antioxidants from vegetable and fruits wastes. Food Chem. 2006; 97: 137-150. doi:10.1016/j.foodchem.2005.03.033.
24. Aravanan P, Jayakumari S. Preliminary Phytochemical studies on Bauhinia racemosa Roots. Research J. Pharm. and Tech. 2021; 14(2):797-799. doi: 10.5958/0974-360X.2021.00139.6.
25. Jenila Jose Jancy V, Kalaichelvan V, Balakrishnan N. Phytochemical Analysis and Anti-Oxidant Activity of various Extracts of Plant Cassia auriculata. Research J. Pharm. and Tech. 2020; 13(12): 6150-6155. doi: 10.5958/0974-360X.2020.01073.2..
26. Sarvan Kumar G, Narender M, Umasankar K, Koteswar Rao GSN, Anka Rao A, Sadhana N. Phytochemical investigation and In vitro Thrombolytic activity of Terminalia pallida Brandis leaves Research J. Pharm. and Tech. 2021; 14(2): 879-882. DOI: 10.5958/0974-360X.2021.00156.6.
27. Jenila Jose Jancy V, Kalaichelvan V, Balakrishnan N. Phytochemical Analysis and Anti-Oxidant Activity of various Extracts of Plant Cassia auriculata. Research J. Pharm. And Tech., 2020; 13(12):6150-6155. doi: 10.5958/0974-360X.2020.01073.2.
28. Kaur and Kapoor, Kaur C, Kapoor H C. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Technol. 2002; 37: 153–161. doi.org/10.1046/j.1365-2621.2002.00552.
29. Chang et al., Chang C, Yang M, Wen, H, Chern. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 2002; 10: 178–182. doi: 10.12691/jfnr-8-11-3.
30. Asha Monica A, Senthilkumar S R. Green synthesis and characterization of Silver nanoparticles from Ocimum basilicum and their Antimicrobial Antioxidant and Anticancer activity. Research J. Pharm. and Tech. 2020; 13(12): 5711-5715.doi: 10.5958/0974-360X.2020.00994.4.
31. Mrinmoy B, Moksood Ahmed L, Priyanka G. Evaluation of Anti-oxidant and Anti-pyretic activity of fruits of Garcinia pedunculata. Research Journal of Pharmacy and Technology. 2021; 14(5): 2635-8. doi: 10.52711/0974-360X.2021.00464 .
32. Amiri H. Essential oils composition and antioxidant properties of three Thymus species. Evid Based Complement Alternat Med., 2012: 1-8. doi: 10.1155/2012/728065.
33. Tiwari SS, Pandey MM, Srivastava S, Rawat AK. TLC densitometric quantification of picrosides (picroside-I and picroside-II) in Picrorhiza kurroa and its substitute Picrorhiza scrophulariiflora and their antioxidant studies. Biomed Chromatogr., 2012; 26: 61-8. doi: 10.1002/bmc.1626.
34. Geetha B, Mahendran S, Shrishailappa B. In-vitro Antioxidant and Cytotoxic properties of Strobilanthes kunthianus. Research Journal of Pharmacy and Technology., 2021; 14(5): 2522-8. doi: 10.52711/0974-360X.2021.00444 .
35. Monica Joicy C, Sivaraj C, Arumugam P. In-vitro Antioxidant, Antidiabetic, Antibacterial and Cytotoxic Activities of Essential Oil extracted from Flowers of Illicium verum L. Research Journal of Pharmacy and Technology., 2021; 14(5): 2452-8. doi: 10.52711/0974-360X.2021.00431.
36. Kumaran A, and Joel Karunakaran R. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT-Food Sci Techno., 2007; 40(2): 344-352. doi.org/10.1016/j.lwt.2005.09.011.
37. Gupta M, Mazumdar U.K, Gomathi P, Ramanathan S.K. Antioxidant and Free Radical Scavenging Activities of Ervatamia coronaria Stapf. Leaves. Iran J Pharm Res., 2004: 2,:119-126. doi.org/10.22037/ijpr.2010.587.
38. Shirwaikar A, Shirwaikar A, Rajendran K, and Punitha I.S.R. In vitro antioxidant studies on the Benzyl Tetra Isoquinoline Alkaloid Berberine. Biol Pharm Bull., 2006; 29(9): 1906-1910. doi: 10.1248/bpb.29.1906.
39. Sundar S, Padmalatha K, Apsana SK, Himaja P, Nandini V, Sirisha D. Antibacterial and Antifungal Activity of Carica papaya L Seed Extracts. Research J. Pharm. and Tech., 2021; 14(2): 1085-1090. doi: 10.5958/0974-360X.2021.00195.5.
40. Ritmaleni, Sardjiman, Indah Purwantini. Antimicrobial Activity of Curcumin Analog PGV-6, HGV-6 and GVT-6. Research J. Pharm. and Tech. 2021; 14(2): 599-604. doi: 10.5958/0974-360X.2021.00107.4.
41. Agati G, Azzarello E, Pollastri S, Tattini M. Flavonoids as antioxidants in plants: location and functional significance. Plant Sci. 2012; 19: 67-76. doi:10.1016/j.plantsci.2012.07.014.
42. S. Balavivekananthan, T. Francis Xavier, S. R. Senthil Kumar, R. Sabitha. Antibacterial activity of Solanum elaeagnifolium Cav. stem and leaf extract against human pathogenic bacteria. Research J. Pharm. and Tech. 2021; 14(3): 1339-1345. doi: 10.5958/0974-360X.2021.00238.9.