Author(s):
Anila Mishra, Zeeshan Fatima, Akash Ved, Sajal Srivastava, Mohammad Yasir
Email(s):
zfatima@amity.edu
DOI:
10.52711/0974-360X.2025.00470 
Address:
Anila Mishra1, Zeeshan Fatima1, Akash Ved2, Sajal Srivastava1, Mohammad Yasir1
1Amity Institute of Pharmacy, Lucknow, Amity University, Uttar Pradesh, Sector -125, Noida - 201313, India.
2Faculty of Pharmacy, Dr. A.P.J. Abdul Kalam Technical University, Lucknow - 226031, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
Molecules containing imidazole possess a diverse array of pharmacological activities. Recently, a new library of 2,4,5-trisubstituted imidazoles was synthesised in our previous study utilising a novel organocatalyzed synthetic pathway. Further, these molecules were screened for in vitro enzymatic activity against target a-glucosidase at different concentrations (0–1000µg/mL) of trisubstituted imidazoles. The absorbance was measured at 405 and 540nm using a multiplate reader, and the percentage of a-glucosidase inhibitory activity with the IC 50 values of 2,4,5-trisubstituted imidazoles was calculated. Among them, compounds 3a, 3b, and 3e were found to have good a-glucosidase inhibition with an IC 50 of 4.8, 5.3, and 4.3µg/mL, respectively, when compared to the standard drug acarbose. Additionally, molecular docking studies were performed to comprehend the molecular interaction between the molecule and imidazole-sensitive hypoglycemic target a-glucosidase (PDB: 3WY1). The majority of 2,4,5-trisubstituted imidazoles showed better binding energy (-7.4 to -8.2Kcal/mol) with the binding pockets of a-glucosidase. Swiss ADME was employed to predict the adsorption, distribution, metabolism, and elimination properties of the synthesised compounds.
Cite this article:
Anila Mishra, Zeeshan Fatima, Akash Ved, Sajal Srivastava, Mohammad Yasir. Unravelling the Potential of Trisubstituted Imidazole Derivatives to Combat Hyperglycemia: An In vitro and In silico Approach. Research Journal of Pharmacy and Technology. 2025;18(7):3262-7. doi: 10.52711/0974-360X.2025.00470
Cite(Electronic):
Anila Mishra, Zeeshan Fatima, Akash Ved, Sajal Srivastava, Mohammad Yasir. Unravelling the Potential of Trisubstituted Imidazole Derivatives to Combat Hyperglycemia: An In vitro and In silico Approach. Research Journal of Pharmacy and Technology. 2025;18(7):3262-7. doi: 10.52711/0974-360X.2025.00470 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-7-50
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