ABSTRACT:
Aim: The purpose of this study was to understand how endogenous and exogenous antioxidants affected BAC invasion, migration, and treatment resistance. Methods: Sorensen Method was used to make a phosphate buffer solution using NaCl, KH2PO4, and Na2HPO4 in distilled water, adjusting the pH to 7.2, and filtering the solution. GSH and CWF were examined for hemolytic potential using blood samples, and their in vitro effects on breast cancer cells were assessed. The viability of the cells was assessed using the MTT test, and Cell lysates were made utilizing the freeze-thaw procedure. To assess the effect of hypoxia on BAC cells, GSH/CWF was combined with the hypoxia inducer CoCl2. Results: The study investigated the effects of CWF and GSH, with and without CoCl2, on VEGF, MMP-09 and the advancement of cells in BAC. The results revealed that both CWF and GSH acted as potent antioxidant agents, decreasing VEGF and MMP-09 expression with an increase in concentration, while CoCl2 was a good hypoxia inducer agent. Under hypoxic conditions, both drugs acted as good antioxidant agents, decreasing cell proliferation. CWF had no significant cytotoxicity effect on BAC up to 50µg/ml, while GSH had the highest cytotoxic effect. All information was examined using one-way ANOVA. Conclusions: Our study provides an important guidance on GSH and CWF, both the antioxidants prevent the invasion of breast cancer cells and migration and increasing their concentration lowers VEGF and MMP-09 protein synthesis. CoCl2 causes hypoxia and enhances the synthesis of VEGF and MMP-09, while GSH and CWF might mitigate its effects. Antioxidants also have anti-cancer properties, making them potentially effective for the purpose of treating breast adenocarcinoma.