Author(s):
Wahyu Widyaningsih, Dwi Utami, Widyanari Febriyana Wulansari, Nafilah Nur Irsyad, Nafila Hana Rosyidawati
Email(s):
wahyu.widyaningsih@pharm.uad.ac.id
DOI:
10.52711/0974-360X.2025.00452 
Address:
Wahyu Widyaningsih*, Dwi Utami, Widyanari Febriyana Wulansari, Nafilah Nur Irsyad, Nafila Hana Rosyidawati
Faculty of Pharmacy, Ahmad Dahlan University, Jl. Prof. Dr. Soepomo, Umbulharjo, 55164, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
These days, inflammation and pain are major health issues. One plant that may have analgesic and anti-inflammatory properties is green algae (Ulva lactuca L). Melatonin and sulfated polysaccharides found in green algae may have analgesic and anti-inflammatory properties. This work aims to explore the potential analgesic and anti-inflammatory properties of water extract from green algae. The chemical stimulation approach was utilized to assess analgesic efficacy in vivo at a concentration of 1% acetic acid. There were four groups of mice. Group (K) 1 was given CMC Na 1% (10mg/ml) as a pain reliever; K2 was given Na Diclofenac 0.13mg/20g BW as a positive control; K3 was given 10.4mg/20g BW of green algae; and K4 was given 20.8mg/20g BW of green algae. The pain response was measured as the total quantity of writhing for five minutes over an hour. We looked at the anti-inflammatory qualities in vitro. Tests for anti-inflammatory activity were conducted both in vivo and in vitro. The red blood cell membrane is kept at 54°C using an induction heating method in the in vitro procedure. 2% carrageenin was utilized as an edema inducer to assess the anti-inflammatory activity in vivo. Four groups of Wistar rats were utilized as test subjects, and each group received a different treatment: group 1 received CMC Na, group 2 received 1.8mg/200gBW of diclofenac sodium, group 3 received 36mg/200 gBW of green algae, and group 4 received 72mg/200gBW of the same medication. The anti-inflammatory activity was assessed by measuring the quantity of rat paw edema at 0, 60, 120, 180, 240, and 300 minutes after an hour of carrageenin treatment. The statistical data analysis was done using SPSS. At a dose of 20.8mg/20gBW, green algae water extract demonstrates 63.70% protection percentage and analgesic efficacy. With an IC-50 of 4.2051 ±0.0797mg/mL, green algae have anti-inflammatory qualities in vitro. The anti-inflammatory potency of green algae was found to be 31.78% at 36mg/200gBW and 19.45% at 72mg/200gBW. According to our research, water extracts of green algae have been shown to have analgesic and anti-inflammatory properties.
Cite this article:
Wahyu Widyaningsih, Dwi Utami, Widyanari Febriyana Wulansari, Nafilah Nur Irsyad, Nafila Hana Rosyidawati. Analgesic and Anti-Inflammatory Activities of Water Extract from Green Algae (Ulva lactuca L.). Research Journal of Pharmacy and Technology. 2025;18(7):3146-2. doi: 10.52711/0974-360X.2025.00452
Cite(Electronic):
Wahyu Widyaningsih, Dwi Utami, Widyanari Febriyana Wulansari, Nafilah Nur Irsyad, Nafila Hana Rosyidawati. Analgesic and Anti-Inflammatory Activities of Water Extract from Green Algae (Ulva lactuca L.). Research Journal of Pharmacy and Technology. 2025;18(7):3146-2. doi: 10.52711/0974-360X.2025.00452 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-7-32
REFERENCES:
1. Sianipar EA, Gunardi SI. Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia. J Herbmed Pharmacol. 2023; 12(3): 407-412. doi:10.34172/jhp.2023.44
2. Manurung NRM, Sumiwi SA. Aktivitas Antiinflamasi Berbagai Tanaman Diduga Berasal Dari Flavonoid. Farmaka. 2016; 14(2): 111-122. doi:10.24198/jf.v14i2.10816
3. Sari DP, Rufaida Z, Lestari SWP. Nyeri Persalinan. E-Book Penerbit STIKes Majapahit. Published online November 10, 2018:1-30.
4. H.M JA, Dwimartyono F, Muliyadi FE, et al. Pola Penggunaan Analgesik Pasien Bedah Orthopedi di Ruang Gawat Darurat Rs. Ibnu Sina Makassar. Fakumi Medical Journal: Jurnal Mahasiswa Kedokteran. 2022; 2(7): 496-503. doi:10.33096/fmj.v2i7.99
5. Mangampa I, Nugroho TE. Pengaruh Pemberian Natrium Diklofenak Dosis 1,4 Mg/Kgbb Dan 2,8 Mg/Kgbb Terhadap Kadar Serum Kreatinin Tikus Wistar. other. Faculty of Medicine; 2015. Accessed June 11, 2024. http://eprints.undip.ac.id/46711/
6. Subekti LW, Nugroho TE. Pengaruh Pemberian Natrium Diklofenak Dosis 1,4 Mg/Kgbb Dan 2,8 Mg/Kgbb Terhadap Kadar Ureum Tikus Wistar. other. Faculty of Medicine. 2015. Accessed June 11, 2024. http://eprints.undip.ac.id/46312/
7. Soleha M, Isnawati A, Fitri N, Adelina R, Soblia HT, Winarsih. Profil Penggunaan Obat Antiinflamasi Nonstreoid di Indonesia | Jurnal Kefarmasian Indonesia. 2018;8(2):109-117.
8. Aravind R, Bindu A r, Bindu K, Kanthlal S, AnilKumar B. Anti-Inflammatory Evaluation of the Cinnamomum malabatrum (Burm. F).Blume Leaves using Carrageenan Induced Rat Paw Oedema Method. Research Journal of Pharmacy and Technology. 2013; 6(7): 746-748.
9. Gobinath T, Kaviya K, Ravichandran S. Chitosan/Ulva lactuca polysaccharide Hydrogel containing copper (II) beneficial for Biofilm-associated wound infection: Formulation Characterizations and in vitro study. Research Journal of Pharmacy and Technology. 2020; 13(9): 4224-4230. doi:10.5958/0974-360X.2020.00746.5
10. Tarocco A, Caroccia N, Morciano G, et al. Melatonin as a master regulator of cell death and inflammation: molecular mechanisms and clinical implications for newborn care. Cell Death Dis. 2019; 10(4): 317. doi:10.1038/s41419-019-1556-7
11. Widyaningsih W, Edityaningrum CA, Ningsih RS, Hasanah K. Effects of Ethanol Extract of Green Algae (Ulva lactuca L) on Histological Description, COX-1 Expression and COX-2 in Ethanol-Induced Rat’s Gastric. Research Journal of Pharmacy and Technology. 2023; 16(2): 727-732. doi:10.52711/0974-360X.2023.00124
12. Ramadhan W, Uju U, Hardiningtyas SD, Pari RF, Nurhayati N, Sevica D. Ekstraksi Polisakarida Ulvan dari Rumput Laut Ulva lactuca Berbantu Gelombang Ultrasonik pada Suhu Rendah : 1. 2022;25(1):132-142. doi:10.17844/jphpi.v25i1.40407
13. Tziveleka LA, Ioannou E, Roussis V. Ulvan, a bioactive marine sulphated polysaccharide as a key constituent of hybrid biomaterials: A review. Carbohydrate Polymers. 2019; 218: 355-370. doi:10.1016/j.carbpol.2019.04.074
14. Peasura N, Laohakunjit N, Kerdchoechuen O, Vongsawasdi P, Chao LK. Assessment of biochemical and immunomodulatory activity of sulphated polysaccharides from Ulva intestinalis. International Journal of Biological Macromolecules. 2016; 91: 269-277. doi:10.1016/j.ijbiomac.2016.05.062
15. Tabarsa M, You S, Dabaghian EH, Surayot U. Water-soluble polysaccharides from Ulva intestinalis: Molecular properties, structural elucidation and immunomodulatory activities. Journal of Food and Drug Analysis. 2018; 26(2): 599-608. doi:10.1016/j.jfda.2017.07.016
16. Utami D, Wahyudi R, Widyaningsih W. The sulphated polysaccharide compounds from green algae (Ulva lactuca L) as a potential natural anti-inflammatory agent based on molecular docking study targeting cyclooxygenase-2 receptor. Pharmaciana. 2023; 13(2): 146-158.
17. Umami M, Pratama IS, Sriasih M. Evaluasi Sifat Fisik dan Skrining Fitokimia Teh Biji Pepaya (Carica papaya L.). Jurnal Kedokteran. 2021; 10(2): 451-455. doi:10.29303/jku.v10i2.522
18. Widyasanti A, Halimah T, Rohdiana D. Ekstraksi Teh Putih Berbantu Ultrasonik pada Berbagai Amplitudo. Jurnal Aplikasi Teknologi Pangan. 2018;7(3). doi:10.14710/baf.%v.%i.%Y.200-214
19. Fadhilla G, Adnyana IK, Chaniago R. Aktivitas Analgetik Ekstrak Etanol Daun Ciplukan (Physalis Peruviana L.) pada Mencit Swiss Webster Jantan dengan Metode Geliat (Sigmund). Jurnal Ilmiah Farmako Bahari. 2020; 11(1): 75-88. doi:10.52434/jfb.v11i1.716
20. Ardina R, Rosalinda S. Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, dan Kombinasi Wright-Giemsa. Jurnal Surya Medika (JSM). 2018; 3(2): 5-12. doi:10.33084/jsm.v3i2.91
21. Kosala K, Widodo MA, Santoso S, Karyono S. In vitro and In vivo Anti-inflammatory Activities of Coptosapelta flavescens Korth Root’s Methanol Extract. J App Pharm Sci. 2018; 8,(9): 042-048. doi:10.7324/JAPS.2018.8907
22. Ariyanti D, Arsita N. Isolasi dan Uji Daya Hambat Bakteri Endofit Alga Hijau (Ulva lactuca) terhadap Bakteri Staphylococcus aureus dan Salmonella thyphii. Jurnal Tambora. 2023; 7(2): 24-32.
23. Muthusamy P, Nivedhitha M, Jayshree N. Analgesic and Anti-Inflammatory Activities of Datura metel Linn. Root in Experimental Animal Models. Research Journal of Pharmacy and Technology. 2010;3(3):897-899.
24. Yazidah N, Fakhruddin F, Jaluri PDC. Pengaruh Pemberian Ekstrak Etanol Batang Karamunting (Rhodomyrtus Tomentosa (W.Ait.) Hassk) sebagai Analgesik terhadap Mencit Putih yang Diinduksi Asam Asetat. Jurnal Kesehatan Borneo Cendekia. 2022;6(1):88-96. doi:10.54411/jbc.v6i1.279
25. Amilia R, Kresnamurti A, Faizah AK. Uji Aktivitas Analgesik Minyak Ikan pada Mencit Putih (Mus musculus) Jantan Galur BALB/c dengan Metode Writhing Test. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia). 2020;17(1):13-21. doi:10.30595/pharmacy.v17i1.5049
26. de Araújo IWF, Rodrigues JAG, Quinderé ALG, et al. Analgesic and anti-inflammatory actions on bradykinin route of a polysulfated fraction from alga Ulva lactuca. Int J Biol Macromol. 2016; 92: 820-830. doi:10.1016/j.ijbiomac.2016.07.094