ABSTRACT:
Many of the vegetable crops contain various health-promoting and bio-pharmaceutical nutrients. Bitter gourd (Momordica charantia L) is one of them, which contains many health-promoting phytomolecules. Each individual phytomolecule is useful for one or more deficiencies or diseases. Bitter gourd consists of more than 60 important phytonutrients, making it a functional food for regulating health. Most importantly, bitter gourd contains charantin, which consists of two glucosides, namely stigmasterol and sitosterol, which are effective against diabetes mellitus type II (DM2). Charantin is a micromolecule, so it is important to extract, quantify and formulate it to achieve an optimal dosage against diabetes. In the present study, the initial focus was on selecting a suitable solvent for maximizing charantin extraction from the dry fruit powder of bitter gourd. extraction with an organic solvent is important, and we have chosen methanol for extraction because it is polar, has a high boiling point, is available, etc. There are different extraction methods like ultra sonication, CO2 extraction, and water bath, but we used the Soxhlet extraction method. All three extracts are quantified by reverse phase high performance liquid chromatography (RP- HPLC). Charantin ranges 196 ug/g to 664 ug/ g, 283 to 927 ug/g and 159 to 780ug/g dry weight in absolute ethanol, absolute methanol and 1:1 mixture of ethanol and methanol respectively. Among these three combinations, methanol was found to be the most suitable solvent for extraction of maximum amount of charantin than ethanol and its combination.
Cite this article:
Gajanan Solunke, Arunava Das. Solvent selection for Extraction of Charantin in bitter gourd and comparative quantification by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Research Journal of Pharmacy and Technology. 2025;18(7): 2957-0. doi: 10.52711/0974-360X.2025.00423
Cite(Electronic):
Gajanan Solunke, Arunava Das. Solvent selection for Extraction of Charantin in bitter gourd and comparative quantification by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Research Journal of Pharmacy and Technology. 2025;18(7): 2957-0. doi: 10.52711/0974-360X.2025.00423 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-7-3
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