Author(s):
Susanti Erikania, Ronnawan Juniatmoko, Arikha A. Susilowati, Tatiana S.Wardani, Rahmat A Hi Wahid
Email(s):
susantierikania@stikes-bhm.ac.id
DOI:
10.52711/0974-360X.2025.00432 
Address:
Susanti Erikania1*, Ronnawan Juniatmoko2, Arikha A. Susilowati1, Tatiana S.Wardani3, Rahmat A Hi Wahid4
1Faculty of Pharmacy, STIKES Bhakti Husada Mulia, Madiun, 63131, East Java, Indonesia.
2Department of Science and Technology, Universitas Islam Negeri Raden Mas Said, Surakarta, 57168, Central Java, Indonesia.
3Department of Pharmacy, Universitas Duta Bangsa Surakarta, Surakarta, 57154, Central Java, Indonesia.
4Department of Pharmacy, Faculty of Science and Technology, Universitas PGRI Yogyakarta, Bantul, 55182, Special Region of Yogyakarta, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 7,
Year - 2025
ABSTRACT:
Antioxidants are compounds that have the ability to bind free radicals, which are known to contribute to the development of cancer, and the accumulation of free radicals in the body can trigger the growth of cancer cells. Numerous flavonoid compounds have been reported to have potent antioxidant activity, and mangosteen leaves contain these compounds, indicating the potential of their development to prevent cancer. Therefore, this study was conducted to determine mangosteen leaves’ antioxidant and cytotoxic activities against HepG-2 liver cancer cells using the ethanol extract of mangosteen leaves obtained by maceration. Cytotoxic activity testing was carried out using the MTT assay with eight different concentrations, namely 100, 50, 25, 12.5, 6.25, 3.125, 1.56, and 0.78 µg/mL. The flavonoid content in the mangosteen leaf extract was determined using UV-Vis spectrophotometry. This study showed that the ethanol extract of mangosteen leaves contained 11.8% flavonoids and exhibited potent antioxidant activity with an IC50 value of 4.09 ± 0.04 ppm. The mangosteen leaf extract demonstrated cytotoxicity against Hep-G2 liver cancer cells, with an IC50 value of 9.277 µg/mL, classifying it as cytotoxic. Based on the findings above, it can be concluded that mangosteen leaf extract contains flavonoids with antioxidant and cytotoxic activities.
Cite this article:
Susanti Erikania, Ronnawan Juniatmoko, Arikha A. Susilowati, Tatiana S.Wardani, Rahmat A Hi Wahid. Antioxidant and Anticancer Activity of Mangosteen (Garcina mangostana L.) Leaf Extract against Human Hepatocellular Carcinoma (HepG-2) Cells. Research Journal of Pharmacy and Technology. 2025;18(7):3017-3. doi: 10.52711/0974-360X.2025.00432
Cite(Electronic):
Susanti Erikania, Ronnawan Juniatmoko, Arikha A. Susilowati, Tatiana S.Wardani, Rahmat A Hi Wahid. Antioxidant and Anticancer Activity of Mangosteen (Garcina mangostana L.) Leaf Extract against Human Hepatocellular Carcinoma (HepG-2) Cells. Research Journal of Pharmacy and Technology. 2025;18(7):3017-3. doi: 10.52711/0974-360X.2025.00432 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-7-12
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