Author(s): Natarajan K, Thayumanavan Tha, Subashkumar R

Email(s): rsubashkumar@gmail.com

DOI: 10.52711/0974-360X.2025.00353   

Address: Natarajan K1, Thayumanavan Tha2, Subashkumar R3*
1Department of Biomedical Engineering, Vinayaka Mission’s Kirupananda Variyar Engineering College, Vinayaka Mission’s Research Foundation (Deemed to be University), Salem – 636308, Tamil Nadu, India.
2Department of Biotechnology, KIT - Kalaignarkarunanidhi Institute of Technology, Coimbatore - 641402, India.
3Department of Biotechnology, Sri Ramakrishna College of Arts and Science, Coimbatore - 641006, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 18,      Issue - 6,     Year - 2025


ABSTRACT:
The tea infusion is a drink that made from the leaves of Camellia sinensis. Numerous chemicals with biological activity can be found in large quantities in tea plants. The objective of current investigation was to characterize the antioxidant, antibacterial, and antifungal activity of methanol extract obtained from the tea leaves. The total phenolic content and total flavonoids in the crude extract were estimated as 55.2 ± 1.14 mg/g and 33.88 ± 0.54 mg/g respectively. The antioxidant activities of the extract from tea leaves were found to vary from 14.16 to 81.66% and the IC50 value was 63.12mg/mL. The antimicrobial activity was very effective against E.coli, Klebsiella sp., Mycobacterium philei and Streptococcus sp., Aspergillus flavus, Collectotrichum sp., Mucor sp., and Penicillium sp. The test organism, Bacillus sp., Salmonella typhi, and Aspergillus niger showed resistance to the methanol extract.


Cite this article:
Natarajan K, Thayumanavan Tha, Subashkumar R. Antioxidant, Antibacterial and Antifungal Activity of Camellia sinensis. Research Journal of Pharmacy and Technology. 2025;18(6):2475-9. doi: 10.52711/0974-360X.2025.00353

Cite(Electronic):
Natarajan K, Thayumanavan Tha, Subashkumar R. Antioxidant, Antibacterial and Antifungal Activity of Camellia sinensis. Research Journal of Pharmacy and Technology. 2025;18(6):2475-9. doi: 10.52711/0974-360X.2025.00353   Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-6-6


REFERENCES:
1.    Sharma VK, Bhattacharya A, Kumar A, Sharma H.K.  Health benefits of tea consumption. Tropical Journal of Pharmaceutical Research. 2007; 6 (3): 785-792.
2.    Chopade VV, Phatak AA, Upaganlawar AB, Tankar AA. Green tea (Camellia sinensis): Chemistry, Traditional, Medicinal uses and its Pharmacological activities- a review. Pharmacognosy Reviews. 2008; Jan; 2(3): 157-162.
3.    Anil P., Mahesh M.P. Global Production and Prospective of Green Tea. Asian J. Management. 2013; 4(4): 297-300.
4.    Gupta S, Chaudhuri T, Seth P, Ganguly DK, Giri AK. Antimutagenic effects of black tea (World Blend) and its two active polyphenols theaflavins and thearubigins in Salmonella assays. Phytother Res. 2002; 16(7): 655-61. doi: 10.1002/ptr.1038.
5.    R. Caroline Jeba, G. Abeetha Sandhya, Niranjan Das, C. Suchoritha Shau, S. Ajith Kumar. Anticancer Activity of Microwave Assisted Polyphenolic Compounds Extracted from Combinations of Curcuma longa and Camellia sinensis against Lung Cancer Cell Line. Research Journal of Pharmacy and Technology. 2023; 16(5): 2192-6. doi: 10.52711/0974-360X.2023.00360.
6.    Yang CS, Lambert JD, Sang S. Antioxidative and anti-carcinogenic activities of tea polyphenols. Arch Toxicol. 2009; Jan; 83(1): 11-21. doi: 10.1007/s00204-008-0372-0.
7.    Dhrubajyoti Sarkar, Subhashis Debnath, Sekhar Kumar Bose. Phytochemical analysis and assessment of Antioxidant properties of black tea extract obtained from Camellia sinensis. Research J. Pharm. and Tech. 2020; 13(10): 4539-4544.
8.    Anita P, Sivasamy S, Madan Kumar PD, Balan IN, Ethiraj S. In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms. J Basic Clin Pharm. 2014; Dec; 6(1): 35-9. doi: 10.4103/0976-0105.145777.
9.    Akroum S. Antifungal activity of Camellia sinensis crude extracts against four species of Candida and Microsporum persicolor. J Mycol Med. 2018; Sep; 28(3): 424-427. doi: 10.1016/j.mycmed.2018.06.003. 
10.    Viol Dhea Kharisma, Sasando Dhohan Kharisma, Arif Nur Muhammad Ansori, Hanny Priskila Kurniawan, Adiana Mutamsari Witaningrum, Amaq Fadholly, Martia Rani Tacharina. Antiretroviral Effect Simulation from Black Tea (Camellia sinensis) via Dual Inhibitors Mechanism in HIV-1 and its Social Perspective in Indonesia. Research J. Pharm. and Tech. 2021; 14(1):455-460.
11.    Shaheen N, Akhter KT, Khan IN, Irfan NM. Total phenol content, Anti-inflammatory, Anti-allergic and Antioxidant activity in differently processed Camellia Sinensis of Bangladesh. Bioresearch Communications. 2022 July; 4(1):456-463.
12.    Sumpio BE, Cordova AC, Berke-Schlessel DW, Qin F, Chen QH. Green tea, the “Asian Paradox”, and cardiovascular disease. J. Am. Coll. Surg. 2006; 202(5): 813-820. doi: 10.1016/j.jamcollsurg.2006.01.018.
13.    Prabhakar Semwal, Taranjeet Kapoor , Rahul Vikram Singh. Comparative Phytochemical Screening of Six Different Plant Species of Uttarakhand Region. Asian J. Pharm. Tech. 2014; 4(2): 50-52.
14.    Cabrera C, Artacho R, Giménez R. Beneficial effects of green tea--a review. J Am Coll Nutr. 2006; 25(2): 79-99. doi: 10.1080/07315724.2006.10719518. 
15.    Kuroda Y, Hara Y. Antimutagenic and anticarcinogenic activity of tea polyphenols. Mutat Res. 1999; Jan; 436(1): 69-97. doi: 10.1016/s1383-5742(98)00019-2.
16.    Wang H, Provan GJ, Helliwell K. Tea flavonoids: Their functions, utilization and analysis. Trends Food. Sci. Technol. 2000 Apr; 11:152-160. doi.org/10.1016/S0924-2244(00)00061-3.
17.    Hodgson JM, Croft KD. Tea flavonoids and cardiovascular health. Mol Aspects Med. 2010; 31(6): 495-502. doi: 10.1016/j.mam.2010.09.004.
18.    Chan EW, Soh EY, Tie PP, Law YP. Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacognosy Res. 2011; Oct; 3(4): 266-72. doi: 10.4103/0974-8490.89748.
19.    Koech KR, Wachira FN, Ngure RM, Orina IA, Wanyoko JK, Bii C, Karori SM. Antifungal activity of crude tea extracts. 2013; May; 8(19): 2086-2089. doi: 10.5897/AJAR2013.6742
20.    Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. J Agric Food Chem. 2003; 51: 609-614. doi: 10.1021/jf020782a.
21.    Robert EB. Method for estimation of tannin in grain sorghum. Agrochemical Journal, 1971; 63(3): 511-12.
22.    Liyana-Pathiranan CM, Shahidi F. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L) as affected by gastric pH conditions. J Agric Food Chem. 2005; 53:2433-2440. doi: 10.1021/jf049320i.
23.    Chan EW, Soh EY, Tie PP, Law YP. Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacognosy Res. 2011; 3(4): 266-72. doi: 10.4103/0974-8490.89748. 
24.    Shovon LS, Prianka S, Nigarin S. In vitro evaluation of phytochemical components and antimicrobial activity of the methanolic extract of Tridax procumbens L. against pathogenic microorganisms. Journal of Pharmacognosy and Phytochemistry. 2016; 5(5): 42-46.
25.    Rababah TM, Hettiarachchy NS, Horax R. Total phenolics and antioxidant activities of fenugreek, green tea, black tea, grape seed, ginger, rosemary, gotu kola, and ginkgo extracts, vitamin E, and tert-butylhydroquinone. J Agric Food Chem. 2004; Aug; 52(16): 5183-6. doi: 10.1021/jf049645z.
26.    Friedman M. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Mol Nutr Food Res. 2007; 51(1): 116-34. doi: 10.1002/mnfr.200600173.
27.    Ng KS, Mohd ZZ, MohdMaidin N, Mamat H, Juhari NH, Zainol MK. High-performance liquid chromatography (HPLC) analysis for flavonoids profiling of Napier grass herbal tea. Food Research. 2021; 5(1): 65-71.
28.    Eka Indra Setyawan, Erna Prawita Setyowati, Abdul Rohman, Akhmad Kharis Nugroho. Simultaneous Determination of Epigallocatechin Gallate, Catechin, and Caffeine from Green Tea Leaves (Camellia sinensis L) Extract by RP-HPLC. Research J. Pharm. and Tech. 2020; 13(3): 1489-1494.
29.    Friedman M. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Mol Nutr Food Res. 2007; Jan; 51(1): 116-34. doi: 10.1002/mnfr.200600173.
30.    Satish S, Mohana DC, Raghavendra MP, Raveesha KA. Antifungal activity of some plant extracts against important seed borne pathogens of Aspergillus sp. Journal of Agricultural Technology. 2007; 3(1): 109-119.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available