ABSTRACT:
Objective: The existing intervention was intended to explore the relative examination of in- vitro thrombolytic potential of active ethanolic fraction each of Cordia macleodii bark (CMB), Cissus repanda root (CRR) and Celosia argentea root (CAR) separately and their combinations in different proportions. Methods: A model of in vitro thrombolysis was utilized to assess the thrombolytic impact of active fractions of chosen herbal medications dissolved in ethanol. The clot lysis effect of 100 µl of each herbal active fraction and equivalent proportions of herbal mixtures from CMB, CRR, and CAR were analyzed by comparing them with 100 µl streptokinase and 100 µl distilled water, used as positive and negative controls, respectively. Percentage of thrombolysis was assessed as a result of weight difference between microcentrifuge tubes before and after clot lysis. Results: Findings from experimental study reveals that streptokinase, positive control was able to dissolve 55.61 % of the clot and sterile distilled water, negative control shows 2.12 % clot lysis. The active ethanolic fraction each of CMB, CRR and CAR on an average were able to dissolve 22.63 %, 22.78 % and 26.63 % of the clot independently. Some combinations of these herbal active fractions in various proportions had been tried for thrombolytic activity, the most efficient combination of CMB, CRR and CAR fractions was found to be in (1:2:1) ratio respectively and was capable to split 51.49 % of clot. Conclusion: From the research findings it was determined that the mixture of active fractions of CMB, CRR and CAR in proportion of (1:2:1) correspondingly possessed significant thrombolytic potential compare to other proportions and individual active fractions of selected crude drugs used under study.
Cite this article:
Bandu M. Kadoo, Prakash R. Itankar. In-vitro Antithrombotic potential of Ethanolic Active Fractions and Herbal Mixture containing Cordia macleodii Hook, Cissus repanda Vahl and Celosia argentea Linn. Research Journal of Pharmacy and Technology. 2025;18(6):2447-3. doi: 10.52711/0974-360X.2025.00349
Cite(Electronic):
Bandu M. Kadoo, Prakash R. Itankar. In-vitro Antithrombotic potential of Ethanolic Active Fractions and Herbal Mixture containing Cordia macleodii Hook, Cissus repanda Vahl and Celosia argentea Linn. Research Journal of Pharmacy and Technology. 2025;18(6):2447-3. doi: 10.52711/0974-360X.2025.00349 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-6-2
7. REFERENCES:
1. Sherwani SK. et al. Thrombolytic Potential of Aqueous and Methanolic Crude Extracts of Camellia sinensis (Green Tea): In vitro study. Journal of Pharmacognosy and Phytochemistry. 2013; 2(1): 125-129. DOI: https://dx.doi.org/10.22271/phyto
2. Olie RH, et al. The coagulation system in atherothrombosis: Implications for new therapeutic strategies. Res Pract Thromb Haemost. 2018; Feb 19; 2(2): 188-198. doi: 10.1002/rth2.12080. PMID: 30046721; PMCID: PMC6055505.
3. Y. Peng, X. et al. Microbial fibrinolytic enzymes: an overview of source, production, properties, and thrombolytic activity in vivo. Applied Microbiology and Biotechnology. 2005; 69(2): 126–132. doi:10.1007/s00253-005-0159-7. Epub 2005 Nov 12.
4. Baig M U, et al. Thrombolytic Therapy. [Updated 2023 Aug 28]. In: Stat Pearls [Internet]. Treasure Island (FL): Stat Pearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK557411.
5. Ali M R, et al. Aspect of thrombolytic therapy: a review. Scientific World Journal. 2014; 2014: 586510. doi: 10.1155/2014/586510. Epub 2014 Dec 10. PMID: 25574487; PMCID: PMC4276353.
6. Md. Ramjan Ali and et.al. Aspect of Thrombolytic Therapy: A Review. Hindawi Publishing Corporation. The Scientific World Journal, Volume 2014, Article ID 586510, 8 pages http://dx.doi.org/10.1155/2014/586510.
7. Li T, Yuan et al. Antithrombotic drugs—pharmacology and perspectives. Coronary artery disease: Therapeutics and drug discovery. 2020: 101-31. https://link.springer. com/chapter/10.1007/978-981-15-2517-9_4
8. Saputri FC, et al. A. Combination of ginger and sappan wood extract effect on in vivo antithrombotic activity test. Journal of Young Pharmacists. 2017; 9(1): S46-S48.
9. Keith Griffiths et al. Food Antioxidants and Their Anti-Inflammatory Properties: A Potential Role in Cardiovascular Diseases and Cancer Prevention. Diseases. 2016; 4(3); 28: https://doi.org/10.3390/diseases4030028
10. Désiré Collen. Coronary Thrombolysis: Streptokinase or Recombinant Tissue-Type Plasminogen Activator?. Ann Intern Med.1990; 112: 529-538. doi:10.7326/0003-4819-112-7-529.
11. Dr. Ved Prakash An overview of some medicinal plants used by tribals of Madhya Pradesh JMPS. 2024; 12(1): 127-129, DOI: https://doi.org/10.22271/plants. 2024.
12. Polhill, R.M. Flora of Tropical East Africa - Moraceae (1st ed.). CRC Press. (1989); 75; 12-19. https://doi.org/10.1201/9781003071976.
13. Ng TB, et al. Anti–human immunodeficiency virus (anti–HIV) natural products with special emphasis on HIV transcriptase inhibitors. Life Science. 1997; 61: 933– 949. doi: 10.1016/s0024-3205(97)00245-2.
14. Manisha J. Ojha et al. Traditional uses, phytochemistry and pharmacology of the medicinal species of the genus Cordia (Boraginaceae), J. of Pharmacy and Pharmacology. 2017; Jul; 69(7): 755-789. doi: 10.1111/jphp. 12715. Epub 2017 Mar 7.
15. Thirupathi, K. et al. A Review of Medicinal Plants of the Genus Cordia: Their Chemistry and Pharmacological Uses. Journal of Natural Remedies. 2008; 8(1): 1–10. https://doi.org/10.18311/jnr/2008/288.
16. Pranati Nayak et al. Ethnobotany, Phytochemistry, Pharmacognostic and Pharma-cological Aspects of Cordia macleodii Hook.f.& Thomson- A review. J. of Non-Timber Forest Products. 2016; 23(2); 67-71. DOI: https://doi.org/10.54207/bsmps2000-2016-J75QE.
17. Ahirwar k et al. Phytopharmacology and applications of cordia macleodii: a miraculous plant. International J. of Biology, Pharmacy and Allied Sciences. 2023; 12(4): 1519-1535; https://doi.org/10.31032/IJBPAS/2023/12.4.7018.
18. Soni P et al. Antivenom potential of ethanolic extract of Cordia macleodii bark against Naja venom. Asian Pac J Trop Biomed. 2014 May; 4(Suppl 1): S449-54. doi: 10.12980/APJTB.4.2014C1048. PMID: 25183127; PMCID: PMC4025313.
19. Harisha C. R.et al. Development of Random Amplified Polymorphic DNA markers for authentification of Cissus repanda vahl. AYU (An International Quarterly Journal of Research in Ayurveda. 2012; 33(2); 279-83. DOI:10.4103/0974-8520.105252.
20. Sachin Bhusari et al. Pharmacognostic and phytochemical investigation of Cissus repanda Vahl leaves. Asian Journal of Pharmacy and Pharmacology. 2019; 5(4): 793-798. DOI: https://doi.org/10.31024/ajpp.2019.5.4.20.
21. Sachin Bhusari et al. Phytochemical Investigation, TLC-HPLC Fingerprinting and Antioxidant Activity of Cissus repanda Roots. Indian Journal of Pharmaceutical Education and Research. 2020; 54(4). DOI: 10.5530/ijper.54.4. 206.
22. Harisha C. R. et al. Pharmacognostical evaluation on roots of Cissus repanda vahl. a folk medicine. Pharmacognosy Journal. 2010; 2(17):17–20. DOI:10.1016/S0975-3575(10)80004-5.
23. Singh V. Medicinal plants and bone healing. Natl J Maxillofac Surg. 2017; 8(1): 4-11. doi: 10.4103/0975-5950.208972. Erratum in: Natl J Maxillofac Surg. 2020; Jul-Dec; 11(2): 309. doi: 10.4103/njms.NJMS_265_20. PMID: 28761270; PMCID: PMC5512407.
24. Shad AA et al. Phytochemical and biological activities of four wild medicinal plants. Scientific World Journal. 2014; 2014: 857363. doi: 10.1155/2014/857363. Epub 2014 Oct 13. PMID: 25374941; PMCID: PMC4211140.
25. Ganapathy A.L. Review on Phytochemical and Pharmacological activities of the genus Cissus Linn. International J. of Pharmaceutical Rsearch. 2017; 9(3). http://www.ijpronline.com/ViewArticleDetail.aspx?ID=983
26. Wang Y. H. et al. Stilbene C- glucoside from Cissus ripens, Journal of Asian Natural Products Res. 2007; 9; 631-636. DOI: 10.1080/10286020600979548.
27. Sundaran J, et al. A short review on pharmacological activity of Cissus quadrangular is. Bioinformation. 2020; 16(8): 579-585. doi: 10.6026/97320630016579. PMID: 33214745; PMCID: PMC7649020.
28. Sarika Sangekar et al. Phytochemical and Taxonomical Studies of Celosia Argentea L. (Amaranthaceae). International Journal of Scientific Research in Science and Technology. 2018; 4(5): 481-486. DOI:10.32628/IJSRST184571.
29. Bapu R. Thorat- Review on Celosia argentea L. Plant. Research Journal of Pharmacognosy and Phytochemistry. 2018; 10(1): 109-119. doi: 10.5958/0975-4385.2018.00017.1.
30. O.D. Adegbaju et al. Proximate, mineral, vitamin and anti-nutrient content of Celosia argentea at three stages of maturity. South African J. of Botany, 2019; 124; 372-379. https://doi.org/10.1016/j.sajb.2019.05.036.
31. Vanitha Varadharaj et al. Phytochemical and Phytotherapeutic Properties of Celosia species- A Review. International Journal of Pharmacognosy and Phytochemical Research. 2017; 9(6): 820-825 DOI:10.25258/phyto.v9i6.8185.
32. Dnyaneshwar Landage et al. Pharmacological Review of Celosia Argentea. International Journal of Advanced Research in Science, Communication and Technology (IJARSCT). 2024; 4(2): 571-578. DOI: 10.48175/IJARSCT-15.
33. R. U. Hamzah. Methanolic extract of Celosia argentea var. crista leaves modulates glucose homeostasis and abates oxidative hepatic injury in diabetic rats. Comp Clin Path. 2018; 27(4): 1065–1071. doi: 10.1007/s00580-018-2702-9.
34. Asha Jadhav et al. Anti-Diabetic Hypolipidemic and Histopathological Analysis of Methanolic Leaf Extract of Celosia argentea on Alloxan Induced Diabetic Rats. International Journal of Pharmaceutical Research and Applications. 2021; 6(1): 857-864 www.ijprajournal.com, DOI: 10.35629/7781-0601857864.
35. Sayanna das et al. Current developments on anti-inflammatory natural medicines. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(8): 61-65. DOI: http://dx.doi.org/10.22159/ajpcr.2018.v11i8.26523.
36. Vetrichelvan T, Jegadeesan M, Devi BA. Anti-diabetic activity of alcoholic extract of Celosia argentea Linn. seeds in rats. Biol Pharm Bull. 2002; 25(4): 526-8. doi: 10.1248/bpb.25.526. PMID: 11995938.
37. Cai Y, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem. 2003; 51(8): 2288-94. doi: 10.1021/jf030045u. PMID: 12670172.
38. Maria G. M. Betalains in Some Species of Amaranthaceae Family: A Review. 2018; 7(4): 53-60. doi: 10.3390/antiox7040053, PMCID: PMC5946119.
39. Azwanida, N.N. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal and Aromatic Plants. 2015; 4: 3. https://doi.org/10.4172/2167-0412.1000196.
40. Matsubara, K. et al. Anticoagulant properties of a sulfated galactan preparation from a marine green alga, Codium cylindricum. International Journal of Biological Macromolecules. 2001; 28(5): 395-399. DOI: 10.1016/s0141-8130(01)00137-4
41. Guglielmone HA, et al. Anticoagulant effect and action mechanism of sulphated flavonoids from Flaveria bidentis. Thromb Res. 2002; 105(2): 183-8. doi: 10.1016/s0049-3848(01)00419-4. PMID: 11958811.
42. Taleb-Contini SH, et al. Antiprotozoal effect of crude extracts and flavonoids isolated from Chromolaena hirsuta (Asteraceae). Phyto her Res. 2004; 18(3): 250-4. doi: 10.1002/ptr.1431. PMID: 15103676.
43. Sanjeev, S. et al. Qualitative and quantitative phytochemical analysis and DPPH radical scavenging activity of different extracts of flower of Bauhinia acuminata linn. International Journal of Current Pharmaceutical Research. 2017; 996): 23-26, doi:10.22159/ijcpr.2017v9i6.23423.
44. Prasad S, et al. Effect of Fagonia arabica (Dhamasa) on in-vitro thrombolysis. BMC Complementary Altern. Med. 2007; 7(1): 36. https://doi.org/10.1186/1472-6882-7-36.
45. Vrutti Patel, et al. Evaluation of in-vitro Thrombolytic activity of methanolic extract of Prunus avium L. Asian J. Res. Pharm. Sci. 2021; 11(1): 41-44. doi: 10.5958/2231-5659.2021.00007.2.
46. Sharmin Reza Choudhury et al. Evaluation of thrombolytic and membrane stabilizing activities of four medicinal plants of Bangladesh; International Journal of Pharmaceutical Sciences and Research. 2023; 4(11): 4223-4227. DOI:10.13040/IJPSR.0975-8232.4(11).4223-27.
47. Ogugofor, M.O., et al. Phytochemical analysis and thrombolytic profiling of Costus afer stem fractions. Futur J Pharm Sci. 2022; 8: 4. https://doi.org/10.1186/s43094-021-00392-3.
48. Antithrombotic Trialists' Collaboration. Collaborative meta-analysis of randomised trials of antiplatelet therapy for prevention of death, myocardial infarction, and stroke in high risk patients. BMJ. 2002; 324(7329): 71-86. doi: 10.1136/bmj.324.7329.71. Erratum in: BMJ 2002 Jan 19;324(7330):141. PMID: 11786451; PMCID: PMC64503.
49. Bottom of Form Wardlaw JM, et al. Thrombolysis for acute ischaemic stroke. Cochrane Database Syst Rev. 2014; 7: CD000213. doi:10.1002/14651858. CD000213. pub3. PMID: 25072528; PMCID: PMC4153726.
50. Madheeh Mohamed Hussain et al. Factors associated with prehospital delay in acute myocardial infarction in Maldives. International Journal of Emergency Medicine. 2023; 16: 1 https://doi.org/10.1186/s12245-023-00503-2.
51. Harvey D. White et al. Thrombolysis for Acute Myocardial Infarction. Circulation. 97(16) https://doi.org/10.1161/01.CIR.97.16.1632.
52. Bhaargavi, V. et al. Thrombolytic activity of herbal mixture containing aqueous crude extracts. International Journal of Research and Development in Pharmacy & Life Sciences. 2016; 5(4): 2264-2268. Retrieved from https://www.ijrdpl.com/index.php/ijrdpl/article/view/248.
53. Shalini H. Kumar et al. Thrombolytic potential of Punica granatum – a study in the rat model. IJPSR. 2016; 7(8): 3348-3354. DOI:10.13040/IJPSR.0975-8232.7(8).3348-54.
54. Jon G. Keevil, et al. Grape Juice, But Not Orange Juice or Grapefruit Juice, Inhibits Human Platelet Aggregation. The Journal of Nutrition. 2000; 130(1): 53-56 https://doi.org/10.1093/jn/130.1.53.
55. Ahirwar k. et al. Phytopharmacology and applications of cordia macleodii: a miraculous plant. IJBPAS. 2023; 12(4): 1519-1535. Https://doi.org/10.31032/ijbpas/2023/12.4.7018.
56. Mahaveer sing et al. A Comprehensive review of phytochemical and pharmacological overview on celosia cristata for future prospective research. 2020; 13(12). DOI: http://dx.doi.org/10.22159/ajpcr.2020.v13i12.38675