Author(s):
Pallavi N. Patil, Ritu M. Gilhotra, Shweta Sharma, Prashant K. Dhakd, Kiran A. Wadkar
Email(s):
reachpallu58@gmail.com
DOI:
10.52711/0974-360X.2025.00310 
Address:
Pallavi N. Patil*1,2, Ritu M. Gilhotra1, Shweta Sharma1, Prashant K. Dhakd, Kiran A. Wadkar3
1School of Pharmacy, Suresh Gyan Vihar University, Mahal Jagatpura, Jaipur, Rajasthan India. 302017.
2Department of Pharmaceutical Chemistry, Dr. J.J. Magdum Trust’s, Dr. J.J. Magdum Pharmacy College, Jaysingpur, Dist - Kolhapur, Maharashtra, India, 416101.
3Department of Pharmacognosy, Teerthankar Education Society’s, Dr. Shivajirao Kadam College of Pharmacy, Kasabe Digraj, Taluka - Miraj, Dist - Sangli, Maharashtra, India.416305.
*Corresponding Author
Published In:
Volume - 18,
Issue - 5,
Year - 2025
ABSTRACT:
Background and purpose: Researchers looked at the HepG2 liver cancer cell line to see whether ethanol and an aqueous extract of Cissuswoodrowii leaves and stems had any anticancer effects. Experimental approach: Using the soxhlet equipment, ethanol and water-based plant extracts were produced for this study. Using the MTT assay, trypan blue, and brine shrimp lethality assay, in-vitro anticancer action of certain plant extracts was investigated at doses ranged from 10 to 100µg/ml against designated cell line. Findings/Results: The anticancer action was achieved with the usage of 5-flurouracil. In comparison to the water-based stem and leaf extracts of CissuswoodrowiiSantapau, ethanolic stem extract had the greatest % of inhibition against the HepG2 cell line, along with effective IC50 values. Conclusion and implication: In conclusion, our results suggest that the 50% ethanol-aqueous stem and leaves extract of Cissuswoodrowii (Stapf Ex T. Cooke) Santapu showed a clear cytotoxic and brine shrimp lethality inductive impact on the human hepatoma (HepG2) cell line, suggesting an anticancer activity.
Cite this article:
Pallavi N. Patil, Ritu M. Gilhotra, Shweta Sharma, Prashant K. Dhakd, Kiran A. Wadkar. In-vitro Anticancer Activity of Ethanol and Aqueous Extract of Leaves and Stem of Cissus woodrowii (Stapf Ex T. Cooke) Santapau against HepG2 Liver Cancer Cell Line. Research Journal of Pharmacy and Technology. 2025;18(5):2164-8. doi: 10.52711/0974-360X.2025.00310
Cite(Electronic):
Pallavi N. Patil, Ritu M. Gilhotra, Shweta Sharma, Prashant K. Dhakd, Kiran A. Wadkar. In-vitro Anticancer Activity of Ethanol and Aqueous Extract of Leaves and Stem of Cissus woodrowii (Stapf Ex T. Cooke) Santapau against HepG2 Liver Cancer Cell Line. Research Journal of Pharmacy and Technology. 2025;18(5):2164-8. doi: 10.52711/0974-360X.2025.00310 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-5-33
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