Author(s):
Rajesh Shenbhakutty Chandrabose, Sardar Khan S, Palanisamy Pethapachetty, Harisankerji M, Brito Raj S, Arun Kumar MS
Email(s):
khansardar25@gmail.com
DOI:
10.52711/0974-360X.2025.00299 
Address:
Rajesh Shenbhakutty Chandrabose1,3, Sardar Khan S2*, Palanisamy Pethapachetty4, Harisankerji M5, Brito Raj S2, Arun Kumar MS6
1Department of Pharmaceutical Analysis, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission's Research Foundation (Deemed to be University), Salem - 636308, Tamil Nadu, India.
2School of Pharmacy, Dhanalakshmi Srinivasan University, Samayapuram, Trichy, 621112, Tamilnadu, India.
3Department of Pharmaceutical Analysis, Sankaralingam Bhuvaneswari College of Pharmacy, Anaikuttam, Sivakasi - 626130, Tamil Nadu, India.
4Department of Pharmaceutics, Vinayaka Mission’s College of Pharmacy, Vinayaka Mission's Research Foundation (Deemed to be University), Salem - 636308, Tamil Nadu, India.
5Beref Pharmaceuticals Private Limited, Madurai - 625008, Tamil Nadu, India.
6Faculty of Pharmacy, Karpagam Academy of Higher Education, Coimbatore, 641021 Tamilnadu, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 5,
Year - 2025
ABSTRACT:
Piroxicam is a prominent pain reliever that's accessible in a wide range of dosage forms such as tablets, ointments, and gel. Formerly, multiple approaches for active therapeutic ingredients and tablet dosage forms have been developed. According to the review of the literature, no approaches for piroxicam gel dosage form have been developed. Piroxicam in gel dosage form was estimated using a Reverse phase high-performance liquid chromatography (RP-HPLC) method that is fast, responsive, reliable, and affordable. The analytical method was developed using a Kromacil C18 column (250 X 4.6mm 5 µ with a 25mM KH2PO4 buffer (pH 3.0) and acetonitrile (ACN) during the mobile phase in a (60:40v/v) ratio at 1.0mL/min. The eluent was detected at 325nm using a UV-Visible detection device. Piroxicam elutes after 5.525 minutes. The suggested approach provides linearity of concentration from 4.00 to 12.01 µg/mL, with an r2 value of 0.9998. According to ICH requirements, this method was evaluated for process accuracy, specificity, linearity, dependability, robustness, and ruggedness.
Cite this article:
Rajesh Shenbhakutty Chandrabose, Sardar Khan S, Palanisamy Pethapachetty, Harisankerji M, Brito Raj S, Arun Kumar MS. Development and Validation of a Robust RP-HPLC Method for the Quantification of Piroxicam in Topical Gel Formulations: Assessing Analytical Performance. Research Journal of Pharmacy and Technology. 2025;18(5):2090-4. doi: 10.52711/0974-360X.2025.00299
Cite(Electronic):
Rajesh Shenbhakutty Chandrabose, Sardar Khan S, Palanisamy Pethapachetty, Harisankerji M, Brito Raj S, Arun Kumar MS. Development and Validation of a Robust RP-HPLC Method for the Quantification of Piroxicam in Topical Gel Formulations: Assessing Analytical Performance. Research Journal of Pharmacy and Technology. 2025;18(5):2090-4. doi: 10.52711/0974-360X.2025.00299 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-5-22
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