Author(s):
Prabhu Vasanthapriya, Neethi Ajith, Thangavelu Ramesh, Muthusamy Thangaraj
Email(s):
coralholders@gmail.com
DOI:
10.52711/0974-360X.2025.00290 
Address:
Prabhu Vasanthapriya, Neethi Ajith, Thangavelu Ramesh, Muthusamy Thangaraj*
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai, Tamil Nadu - 608502, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 5,
Year - 2025
ABSTRACT:
Four bacterial strains such as Pseudomonas stutzeri LE01, Bacillus firmus LE02, Cytobacillus firmus LE03, and Bacillus licheniformis LE04 were isolated from Leiognathus equula and screened for enzyme production. Among these strains, B. firmus LE02 was found to be a potential one for producing amylase, protease, lipase and cellulase with maximum zone of clearance. The optimum condition for protease production by B. firmus LE02 was salinity 1%, pH 9 and lactose 1%. The protease produced by B. firmus LE02 was 0.045mg/ml in comparison with standard proteinase K. Further optimization is required for the large scale enzyme production and characterization is also warranted for effective application.
Cite this article:
Prabhu Vasanthapriya, Neethi Ajith, Thangavelu Ramesh, Muthusamy Thangaraj. Optimization of Extracellular Alkaline Protease Production by Bacillus firmus LE02, Isolated from a Ponyfish, Leiognathus equula. Research Journal of Pharmacy and Technology. 2025;18(5):2032-6. doi: 10.52711/0974-360X.2025.00290
Cite(Electronic):
Prabhu Vasanthapriya, Neethi Ajith, Thangavelu Ramesh, Muthusamy Thangaraj. Optimization of Extracellular Alkaline Protease Production by Bacillus firmus LE02, Isolated from a Ponyfish, Leiognathus equula. Research Journal of Pharmacy and Technology. 2025;18(5):2032-6. doi: 10.52711/0974-360X.2025.00290 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-5-13
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