Author(s):
Annapurna Anusha Kotwala, Komala. M
Email(s):
anusshah.seethala@gmail.com , komala.pharmacy@gmail.com
DOI:
10.52711/0974-360X.2025.00216 
Address:
Annapurna Anusha Kotwala1, Komala. M2*
1,2Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Chennai - 600117, Tamilnadu, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 4,
Year - 2025
ABSTRACT:
One antineoplastic analogue of purine nucleosides from the second generation is clofarabine. This study aimed to produce clofarabine-loaded chitosan nanoparticles as a possible drug delivery vehicle for anticancer11,12. The design and development of innovative drug delivery systems, mostly based on nanotechnology, has become necessary due to the constraints associated with the conventional treatment of cancer. Chitosan-based nanoparticles have caught the interest of researchers as one of the many innovative medication delivery strategies for the treatment of cancer. Chitosan is a polycationic polymer that has several properties, including biocompatibility, biodegradability, and non-toxicity, which makes it the perfect polymer for creating drug delivery systems. Chitosan was used to cover the prepared nano-emulzion of CLOFARABINE. A two-step emulsification procedure was used in the current investigation to create chitosan-decorated multiple Nano emulzions (MNEs). A total of five MNE sample formulations were created and assessed. Entrapment efficiency (EE), stability, zeta potential, globule size, PDI and invitro drug release tests were used to characterize the produced formulations. The manufactured MNE displayed regulated drug release. The chitosan ornamentation used to create sustained release for the MNE. Additionally, studies were conducted on conjugated nanoparticles' cytotoxicity.
Cite this article:
Annapurna Anusha Kotwala, Komala. M. Fabrication and Cytotoxic activity of Clofarabine Nano Emulzion Decorated with Chitosan on MCF-7 cell linings. Research Journal of Pharmacy and Technology. 2025;18(4):1507-4. doi: 10.52711/0974-360X.2025.00216
Cite(Electronic):
Annapurna Anusha Kotwala, Komala. M. Fabrication and Cytotoxic activity of Clofarabine Nano Emulzion Decorated with Chitosan on MCF-7 cell linings. Research Journal of Pharmacy and Technology. 2025;18(4):1507-4. doi: 10.52711/0974-360X.2025.00216 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-4-7
REFERENCES:
1. Katzung, B. G., and Trevor, A. J. Katzung and Trevor’s pharmacology: Examination and Board Review, 12E. The McGraw-Hill Companies, Inc.2019
2. Rutuja Sawant, Aloka Baghkar, Sanjukta Jagtap, Lina Harad, Anagha Chavan, Nilofar A. Khan, Rupali P. Yevale, Mohan K. Kale. A Review on - Herbs in Anticancer. Asian J. Res. Pharm. Sci. 2018; 8(4): 179-184. doi: 10.5958/2231-5659.2018.00031.0
3. Salla Pujitha, A. Jaswanth, A. Ganesh, Irfana Begum, Noor Jahan. Evaluation of Anticancer activity of Maytenus emarginata on Ehrlich’s Ascites Carcinoma treated mice. Asian J. Res. Pharm. Sci. 2018; 8(3): 133-136. doi: 10.5958/2231-5659.2018.00023.1
4. John. D. Milton, M. Alvin Jose. Anticancer Activity of Ethyl Acetate Root Extract of Xanthium strumarium Linn. in N-Nitroso N-Methyl Urea Induced Mammary Carcinoma in Female Sprague-Dawley Rats. Research J. Pharm. and Tech. 2016; 9(8): 1115-1118. doi: 10.5958/0974-360X.2016.00211.0 Available on: https://rjptonline.org/AbstractView.aspx?PID=2016-9-8-20
5. Nandhini S, Radha R, Vadivu R. Docking of Hematoporphyrin on Various Anticancer Drugs Targeting Enzymes. Asian J. Pharm. Res. 2016; 6(3): 123-130. doi: 10.5958/2231-5691.2016.00019.8
6. Dewi, STR, Karim D,. Damaris. Identification Of Content Of Nggorang Leaves (Salvia Occindentalis Sw) using Spectrophotometer Gc-Ms. Pharm Media 2020; 16 No.2, Department Pharmacy Polytechnic Health Makassar Ministry of Health, https://doi.org/10.32382/mf.v16i2.1806
7. Kumari, Divya Sharma, Arjun Singh. In-vivo Studies conducted following the success In-vitro and Dissemination of Anticancer Clinical Trials. Asian Journal of Research in Chemistry. 2024; 17(1): 50-4. doi: 10.52711/0974-4150.2024.00010 Available on: https://www.ajrconline.org/AbstractView.aspx?PID=2024-17-1-10
8. Dewi, STR. Profile Expression Toll like Receptor 4 On exposed mice _ Salmonella typhi given _ Extract Leaf Nggorang (Salvia occidentalis Sw.) (mechanism preventive And Curative) 2020. Department Pharmacy Polytechnic Health Makassar Ministry of Health
9. Akshay R. Yadav, Shrinivas K. Mohite. Anticancer Activity of Psidium guajava Leaf Extracts on Breast Cancer Cell Line. Res. J. Pharma. Dosage Forms and Tech. 2020; 12(4):298-300
10. M Lavanya, Asish Bhaumik, A Gopi Reddy, Ch Manasa, B Kalyani, S Sushmitha. Evaluation of Anticancer Activity of Ethanolic and Ethylacetoacetate Extracts of Sweet Cherry Against Human Breast Cancer Cell Line MCF-7. Research J. Pharmacology and Pharmacodynamics. 2016; 8(2): 65-70 doi: 10.5958/2321-5836.2016.00012.4 Available on: https://rjppd.org/AbstractView.aspx?PID=2016-8-2-4
11. Sasikala M, Sundaraganapathy R, Mohan S. MTT Assay on Anticancer Properties of Phytoconstituents from Ipomoea aquatica forsskal using MCF–7 cell lines for breast cancer in Women. Research J. Pharm. and Tech. 2020; 13(3): 1356-1360. doi: 10.5958/0974-360X.2020.00250.4 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-3-55
12. S. Mohanasundaram, N. Rangarajan, V. Sampath, K. Porkodi, M.V. Dass Prakash, N. Monicka. GC-MS Identification of Anti-inflammatory and Anticancer Metabolites in Edible Milky White Mushroom (Calocybe indica) against Human Breast Cancer (MCF-7) Cells. Research Journal of Pharmacy and Technology. 2021; 14(8): 4300-6. doi: 10.52711/0974-360X.2021.00747 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-8-53
13. Eva Kholifah, Nurul Insani. Anticancer activity of Acmella uliginosa against WiDr colon cancer cell line and T47D breast cancer cell line. Research Journal of Pharmacy and Technology. 2023; 16(2): 640-4. doi: 10.52711/0974-360X.2023.00109 Available on: https://rjptonline.org/AbstractView.aspx?PID=2023-16-2-28
14. Emanuela Giacomelli, Samuel Bertrand, Andreas Nievergelt, Vincent Zwick, Claudia Simoes-Pires, Laurence Marcourt, Elisabeth Rivara-Minten, Muriel Cuendet, Angela Bisio, Jean-Luc Wolfender, Cancer chemopreventive diterpenes from Salvia corrugata, Phytochemistry. 2013; 96: 257-264, https://doi.org/10.1016/j.phytochem.2013.09.011.
15. Liu, L., Jia, J., Zeng, G., Zhao, Y., Qi, X., He, C., Guo, W., Fan, D., Han, G., and Li, Z. Studies on immunoregulatory and anti-tumor activities of a polysaccharide from Salvia miltiorrhiza Bunge. Carbohydrate polymers, 2013; 92(1): 479–483. https://doi.org/10.1016/j.carbpol.2012.09.061
16. Shaheen, Usama and Hussain, Mohammed and Ammar, Hassan. (). Cytotoxicity and Antioxidant Activity of New Biologically Active Constituents from Salvia Lanigra and Salvia Splendens. Pharmacognosy Journal. 2011; 3: 36–48. 10.5530/pj.2011.21.7.
17. Choudhary, Muhammad and Hussain, Amjad and Adhikari, Achyut and Marasini, Bishnu and Sattar, Samina and Wahab, Atiatul and Hussain, Nusrat and Ayatollahi, Seyed Abdulmajid and Rahman, Atta. Anticancer and α-chymotrypsin inhibiting diterpenes and triterpenes from Salvia leriifolia. Phytochemistry Letters. 2013; 6: 139–143. 10.1016/j.phytol.2012.11.010.
18. Cheung, David and Koon, Chi-Man and Wat, Elaine and Ko, Erik and Chan, Judy and Yew, David and Leung, Ping-Chung and Chan, Wai-Yee and Lau, Clara and Fung, Kwok-Pui. A herbal formula containing roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages through inhibition of nuclear factor κB (NFκB) pathway. Journal of Ethnopharmacology. 2012; 145: 10.1016/j.jep.2012.12.011.
19. Parsaee, H., Asili, J., Mousavi, S. H., Soofi, H., Emami, S. A., and Tayarani-Najaran, Z. (). Apoptosis Induction of Salvia chorassanica Root Extract on Human Cervical Cancer Cell Line. Iranian journal of pharmaceutical Research: IJPR. 2013; 12(1): 75–83.
20. Gao, H., Sun, W., Zhao, J., Wu, X., Lu, J. J., Chen, X., Xu, Q. M., Khan, I. A., and Yang, S. Tanshinones and diethyl blechnics with anti-inflammatory and anti-cancer activities from Salvia miltiorrhiza Bunge (Danshen). Scientific reports. 2016; 6: 33720. https://doi.org/10.1038/srep33720
21. Lisda, L., Dewi, STR, and Pakadang, SR.Antihyperglycemic Effectiveness of Salvia Leaf Extract (Salvia occidentalis Sw.) Against Mice (Mus musculus). JECP. 2022. https://doi.org/10.52365/jecp.v2i2.429
22. Salsabila Anggi S. Activity Antibacterial Salvia leaves (Salvia occidentalis sw.) in hinder Escherichia coli and Salmonella thypi, Thesis Major Pharmacy Poltekkes Makassar Ministry of Health. 2022
23. Dewi ST, Sabir M, Pakadang SR, Kamal SE, Sinala S. Anti-Cancer Potential of Nggorang Leaves Extract (Salvia Occidentalis SW.) as a Protein P53 Supressor in T47D Cells. Pharmacognosy Journal. 2021;13(4):1036-1045.
24. Brindisi, M., Bouzidi, C., Frattaruolo, L., Loizzo, M. R., Tundis, R., Dugay, A., Cappello, M. S. Chemical profile, antioxidant, anti-inflammatory, and anti-cancer effects of italian salvia rosmarinus spenn. Methanol leaves extracts. Antioxidants. 2020; 9(9): 1–21. https://doi.org/10.3390/antiox9090826
25. Eltawaty, S. I., Yagoub, S. O., Shouman, S. A., Ahmed, A., and Omer, F. A. Anticancer Effects of Methanol Extract of Libyan Salvia Fruticosa Mill on Mcf7, T47D and (Mda-Mb-468) Breast Cells Lines. 2020; 7(2): 165–169.
26. Han, H., Qian, C., Zong, G., Liu, H., Wang, F., Tao, R., Lu, Y. Systemic pharmacological verification of Salvia miltiorrhiza-Ginseng Chinese herb pair in inhibiting spontaneous breast cancer metastasis. Biomedicine and Pharmacotherapy, 2022; 156(August): 113897. https://doi.org/10.1016/j.biopha.2022.113897
27. Vantangoli, M. M., Madnick, S. J., Huse, S. M., Weston, P., and Boekelheide, K. (2015). MCF-7 Human Breast Cancer Cells Form Differentiated Microtissues in Scaffold-Free Hydrogels. PloS one, 10(8), e0135426. https://doi.org/10.1371/journal.pone.0135426
28. Freshney. R1, Biology Of Cultured Cells In : Culture Of Animal Cells AManual Of Basic Teachnique. Press Hal. 2000: 157- 162
29. Hietanen Sakari, Sonia Lain, Eberhard Krausz, Christine Blattner, and David P. Lane,.Activation of p53 in cervical carcinoma cells by small molecules. Journal PNAS. 2009; 97(15): hal. 8501-8506
30. Prayong, P., Barusrux, S., Weerapreeyakul, N. Cytotoxic activity screening of some indigenous Thai plants, Fitoterapia, 2008; 79: 598-601
31. Kopustinskiene, D. M., Jakstas, V., Savickas, A., and Bernatoniene, J. Flavonoids as Anticancer Agents. Nutrients. 2020; 12(2): 457. https://doi.org/10.3390/nu12020457
32. Ozaki, T., and Nakagawara, A. Role of p53 in Cell Death and Human Cancers. Cancers. 2011; 3(1): 994–1013. https://doi.org/10.3390/cancers3010994
33. Hanahan D, Weinberg R.A. Hallmark of Cancer: The Next generation. Cell 144, March 4, 2011. Elsevier Inc. Jumar, 2010. Entomologi Pertanian, Penerbit Rineka Cipta: Jakarta
34. Chen J. The Cell-Cycle Arrest and Apoptotic Functions of p53 in Tumor Initiation and Progression. Cold Spring Harbor Perspectives in Medicine. 2016; 6(3): a026104. https://doi.org/10.1101/cshperspect.a026104
35. Shenouda, S. M., and Vita, J. A. Effects of flavonoid-containing beverages and EGCG on endothelial function. Journal of the American College of Nutrition. 2007; 26(4): 366S–372S. https://doi.org/10.1080/07315724.2007.10719625
36. Dewi STR, Sabir M, Pakadang SR, Kamal SE, Sinala S. Anti-Cancer Potential of Nggorang Leaves Extract (Salvia Occidentalis SW.) as a Protein P53 Supressor in T47D Phcogj.com Cells. Pharmacogn J. 2021; 13(4): 1036-1045.