Author(s):
Gulzira Yernazarova, Aliya Ramazanova, Svetlana Turasheva, Danagul Mukasheva, Madina Amangeldinova, Saltanat Mamanova, Gulnur Omarova, Nurken Aimukhanov, Ishmam Arabi, Faisal Almalki, Sarkar Kawsar, Taibi Ben Hadda
Email(s):
gulzira.yernazarova@bk.ru
DOI:
10.52711/0974-360X.2025.00266 
Address:
Gulzira Yernazarova1*, Aliya Ramazanova2, Svetlana Turasheva1, Danagul Mukasheva3, Madina Amangeldinova1,4, Saltanat Mamanova3, Gulnur Omarova5, Nurken Aimukhanov6, Ishmam Arabi7, Faisal Almalki8, Sarkar Kawsar9, Taibi Ben Hadda10*
1Al Farabi Kazakh National University, Almaty 050040, Republic of Kazakhstan.
2Kazakh National Women‘s Teacher Training University, Almaty 050000, Republic of Kazakhstan.
3Zhetysu University named after I. Zhansugurov, Taldykorgan 040009, Republic of Kazakhstan.
4Institute of Genetics and Physiology, Almaty 050040, Republic of Kazakhstan.
5Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Republic of Kazakhstan.
6Nazarbayev Intellectual School of Physics and Mathematics, Uralsk 090000, Republic of Kazakhstan.
7Green University of Bangladesh, Dhaka 1207, Bangladesh.
8Umm Al-Qura University, Makkah 21955, Saudi Arabia.
9University of Chittagong, Chittagong-4331, Bangladesh.
10Euromedeterranian University of Fes (UEMF), Meknes
Published In:
Volume - 18,
Issue - 4,
Year - 2025
ABSTRACT:
Nutrients produce various secondary metabolites, including biologically active compounds such as antifungals. The composition of E. Crassipes aquatic plant contains biologically active substances. In this article, the composition of substances in E. Crassipes was identified using IR, 1H, and 13C-NMR methods. The identified substances were characterized for biological activity using the PASS online program. This was based on their molecular structure. As a result of the program, compounds showing activity against light, viruses, earwigs, and bacteria were developed. The program's scope included testing the substances' antimicrobial activity using agar diffusion assay in experiments. Substances were extracted from the aquatic plant via chromatography-mass spectrometry, and their activity against earwigs was examined. The study revealed efficacy against Candida albicans and Candida utilis earwigs.
Cite this article:
Gulzira Yernazarova, Aliya Ramazanova, Svetlana Turasheva, Danagul Mukasheva, Madina Amangeldinova, Saltanat Mamanova, Gulnur Omarova, Nurken Aimukhanov, Ishmam Arabi, Faisal Almalki, Sarkar Kawsar, Taibi Ben Hadda. Research Journal of Pharmacy and Technology. 2025;18(4):1861-1. doi: 10.52711/0974-360X.2025.00266
Cite(Electronic):
Gulzira Yernazarova, Aliya Ramazanova, Svetlana Turasheva, Danagul Mukasheva, Madina Amangeldinova, Saltanat Mamanova, Gulnur Omarova, Nurken Aimukhanov, Ishmam Arabi, Faisal Almalki, Sarkar Kawsar, Taibi Ben Hadda. Research Journal of Pharmacy and Technology. 2025;18(4):1861-1. doi: 10.52711/0974-360X.2025.00266 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-4-57
REFERENCES:
1. Namdeo AG. Plant cell elicitation for production of secondary metabolites: a review. Pharmacognosy Reviews. 2007; 1(1):69-79.
2. Ravichandran K, Palaniraj R, Saw NMMT, Gabr AMM, Ahmed AR, Knorr D, Smetanska I. Effects of different encapsulation agents and drying process on stability of betalain extracts. Journal of Food Science and Technology. 2014; 51: 2216-21. doi: 10.1007/s13197-012-0728-6
3. Alfermann AW, Petersen M. Natural products formation by plant cell biotechnology. Plant Cell, Tissue and Organ Culture. 1995; 43:199-205. doi: 10.1007/BF00052176
4. Filova A. Production of secondary metabolites in plant tissue cultures. Research Journal of Agricultural Science. 2014; 46(1):236-45.
5. Vamanu E, Nita S. Antioxidant capacity and the correlation with major phenolic compounds, anthocyanin, and tocopherol content in various extracts from the wild edible Boletus edulis mushroom. BioMed Research International. 2013: 313905. doi: 10.1155/2013/313905
6. Bulgakov V, Vereshchagiona YV, Veremeichik GN. Anticancer polyphenols from cultured plant cells: production and new bioengineering strategies. Current Medicinal Chemistry. 2018; 25(36):4671-92. doi: 10.2174/0929867324666170609080357
7. Huang WY, Cai YZ, Zhang YB. Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. Nutrition and Cancer. 2009; 62(1):1-20. doi: 10.1080/01635580903191585
8. Georgiev MI, Weber J, Maciuk J. Bioprocessing of plant cell cultures for mass production of targeted compounds. Applied Microbiology and Biotechnology. 2009; 83:809-23. doi: 10.1007/s00253-009-2049-x
9. Murthy HN, Dandin VS, Zhong JJ, Paek KY. Strategies for enhanced production of plant secondary metabolites from cell and organ cultures. In Production of biomass and bioactive compounds using bioreactor technology, edited by Paek KY, Murthy H, Zhong JJ. Springer, Dordrecht. 2014; 471-509. doi: 10.1007/978-94-017-9223-3_20
10. Moreno PRH, van der Heijden R, Verpoorte R. Cell and tissue cultures of Catharanthus Roseus: a literature survey. Plant Cell, Tissue and Organ Culture. 1995; 42(1): 1-25. doi: 10.1007/BF00037677
11. Weathers PJ, Towler MJ, Xu JF. Bench to batch: advances in plant cell culture for producing useful products. Applied Microbiology and Biotechnology. 2010; 85: 1339-51. doi: 10.1007/s00253-009-2354-4
12. Patil RH, Patil MP, Maheshwari VL. Extraction and isolation of secondary metabolites from apocynaceae plants. In Apocynaceae plants. Springer, Singapore. 2023; 37-50. doi: 10.1007/978-981-99-5406-3_4
13. Acheampong EO. Types of flavonoids and their role in plant development. GRIN Verlag. 2023.
14. Ben Bakrim W, Ezzariai A, Karouach F, Sobeh M, Kibret M, Hafidi M, Kouisni L, Yasri A. Eichhornia crassipes (Mart.) Solms: a comprehensive review of its chemical composition, traditional use, and value-added products. Frontiers in Pharmacology. 2022; 13: 842511. doi: 10.3389/fphar.2022.842511
15. Ahmad A, Khan MS. Medicinal plants as potential antifungal agents: current status and future perspectives. Journal of Plant Pathology & Microbiology. 2019; 10(4): 469.
16. Yernazarova GI, Ramazanova AA, Turasheva SK, Almalki FA, Hadda TB, Orazova SB, Madenova AK, Admanova GB, Korul'kin DYu, Sabdenalieva GM, Naimi S, Bukharbayeva Z, Amangeldinova ME. Extraction, purification and characterisation of four new alkaloids from the water plant pistia stratiotes: POM analyses and identification of potential pharmacophore sites. Research Journal of Pharmacy and Technology. 2023; 16(7): 3410-6. doi: 10.52711/0974-360X.2023.00564
17. Muzychkina RA, Korulkin DYu, Abilov JA. Kachestvennyy i kolichestvennyy analiz osnovnykh grupp BAV v lekarstvennom rastitel'nom syr'ye i fitopreparatakh [Qualitative and quantitative analysis of the main groups of BAS in medicinal plant raw materials and phytopreparations]: Monograph. Kazakh University, Almaty. 2004; 288 p.
18. Arabi II, Kawsar SMA. In silico investigations of some carbohydrate derivatives: pass prediction, ADMET, QSAR and molecular docking studies against Pseudomonas aeruginosa. Philippine Journal of Science. 2023; 152(4): 1397-412.
19. Islam S, Hosen MA, Ahmad S, ul Qamar MT, Dey S, Hasan I, Fujii Y, Ozeki Y, Kawsar SMA. Synthesis, antimicrobial, anticancer activities, PASS prediction, molecular docking, molecular dynamics and pharmacokinetic studies of designed methyl α-D-glucopyranoside esters. Journal of Molecular Structure. 2022; 1260: 132761. doi: 10.1016/j.molstruc.2022.132761
20. Lagunin A, Stepanchikova A, Filimonov D, Poroikov V. PASS: prediction of activity spectra for biologically active substances. Bioinformatics. 2000; 16(8): 747-8. doi: 10.1093/bioinformatics/16.8.747
21. Maharani MG, Lestari SR, Lukiati B. Molecular docking studies flavonoid (Quercetin, Isoquercetin, and Kaempferol) of single bulb garlic (Allium sativum) to inhibit lanosterol synthase as antihypercholesterol therapeutic strategies. AIP Conference Proceedings. 2021; 2231:040021. doi: 10.1063/5.0002531
22. Filimonov DA, Lagunin AA, Gloriozova TA, Rudik AV, Druzhilovskii DS, Pogodin PV, Poroikov VV. Prediction of the biological activity spectra of organic compounds using the pass online web resource. Chemistry of Heterocyclic Compounds. 2014; 50(3):444-57. doi: 10.1007/s10593-014-1496-1
23. Hassan SA, Aziz DM, Abdullah MN, Bhat AR, Dongre RS, Hadda TB, Almalki FA, Kawsar SMA, Rahiman AK, Ahmed S, Abdellattif MH, Berredjem M, Sheikh SA, Jamalis J. In vitro and in vivo evaluation of the antimicrobial, antioxidant, cytotoxic, hemolytic activities and in silico POM/DFT/DNA-binding and pharmacokinetic analyses of new sulfonamide bearing thiazolidin-4-ones. Journal of Biomolecular Structure and Dynamics. 2024; 42(7):3747-63. doi: 10.1080/07391102.2023.2226713
24. Sana T, Zehra S, Khan M, Siddiqui BS, Simjee SU, Begum S, Hadda TB. Urease and human glioblastoma (U87) cells growth inhibitory iridoid glycoside acetates from Nyctanthes arbor-tristis Linn. Natural Product Research. 2024; 1-9. doi: 10.1080/14786419.2024.2340059
25. Hosen MI, Mukhrish YE, Jawhari AH, Celik I, Erol M, Abdallah EM, Al-Ghorbani M, Baashen M, Almalki FA, Laaroussi H, Ben Hadda T, Kawsar SMA. Design, synthesis, in silico and POM studies for the identification of the pharmacophore sites of benzylidene derivatives. Molecules. 2023; 28:2613. doi: 10.3390/molecules28062613
26. Prasetyo WE, Kurniansyah V, Firdaus M, Wibowo FR, Ben Hadda T, Almalki FA, Ahmed SA, Kusumaningsih T, Free radical scavenging behaviour of the plant-beneficial secondary metabolite 2,4-diacetylphloroglucinol derivatives under physiological conditions: a joint experimental and theoretical study. Journal of Molecular Structure, 2024; 1302:137498. doi: 10.1016/j.molstruc.2024.137498
27. Kayes MR, Saha S, Alanazi MM, Ozeki Y, Pal D, Ben Hadda T, Legssyer A, Kawsar SMA. Macromolecules: synthesis, antimicrobial, POM analysis and computational approaches of some glucoside derivatives bearing acyl moieties. Saudi Pharmaceutical Journal. 2023; 31(11): 101804. doi: 10.1016/j.jsps.2023.101804
28. Kawsar SMA, Hosen MA, Ahmad S, El Bakri Y, Laaroussi H, Ben Hadda T, Faisal A. Almalki FA, Ozeki Y, Goumri-Said S. Potential SARS-CoV-2 RdRp inhibitors of cytidine derivatives: molecular docking, molecular dynamic simulations, ADMET, and POM analyses for the identification of pharmacophore sites. PLoS ONE. 2022; 17(11):e0273256. doi: 10.1371/journal.pone.0273256
29. Bogoyavlenskiy A, Alexyuk M, Alexyuk P, Berezin V, Almalki FA, Ben Hadda T, Alqahtani AM, Ahmed SA, Dall’Acqua S, Jamalis J. Computer analysis of the inhibition of ACE2 by flavonoids and identification of their potential antiviral pharmacophore site. Molecules. 2023; 28(9):3766. doi: 10.3390/molecules28093766
30. Suleiman M, Almalki FA, Ben Hadda T, Kawsar SMA, Chander S, Murugesan S, Bhat AR, Bogoyavlenskiy A, Jamalis J. Recent progress in synthesis, POM analyses and SAR of coumarin-hybrids as potential anti-HIV agents - a mini review. Pharmaceuticals. 2023; 16(11):1538. doi: 10.3390/ph16111538
31. Chalkha M, Chebbac K, Hassan N, Ben Hadda T, Houari GA, Augustyniak M, Aboul Soud MAM, Yazidi ME. In vitro and in silico evaluation of the antimicrobial and antioxidant activities of spiropyrazoline oxindole congeners. Arabian Journal of Chemistry. 2024; 17:105465. doi: 10.1016/j.arabjc.2023.105465
32. Salih RH, Hasan AH, Hussen NH, Hawaiz FE, Ben Hadda T, Jamalis J, Almalki FA, Adeyinka AS, Coetzee L-C, Oyebamiji AK. Thiazole-pyrazoline hybrids as potential antimicrobial agent: synthesis, biological evaluation, molecular docking, DFT studies and POM analysis. Journal of Molecular Structure. 2023; 1282:135191-208. doi: 10.1016/j.molstruc.2023.135191
33. Bouchareb F, Berredjem M, Dehmchi DA, Kadri R, Kadri M, Ferkous H, Mansouri A, Bouyegh S, Ahmed SA, Ben Hadda T, Bahadi R. Synthesis, characterization, DFT/M06 studies, NBO, QTAIM and RDG analyses of new copper (II) complexes with bis-phosphonamide obtained under microwave irradiation, Journal of Molecular Structure. 2023; 1294(7):136503. doi: 10.1016/j.molstruc.2023.136503
34. Hassan SA, Aziz DM, Abdullah MN, Bhat AR, Dongre RS, Ahmed S, Rahiman AK, Ben Hadda T, Berredjem M, Jamalis J. Design and synthesis of oxazepine derivatives from sulfonamide Schiff bases as antimicrobial and antioxidant agents with low cytotoxicity and hemolytic prospective. Journal of Molecular Structure. 2023; 1292:136121. doi: 10.1016/j.molstruc.2023.136121
35. Hosen MA, Qais FA, Chtita S, Rahman IA, Almehdi AM, Ali F, Almalki FA, Hadda TB, Laaroussi H, Kawsar SMA. In silico and POM analysis for potential antimicrobial agents of thymidine analogs by using molecular docking, molecular dynamics and ADMET profiling. Nucleosides Nucleotides Nucleic Acids. 2023; 42(11): 877-918. doi: 10.1080/15257770.2023.2215839
36. Maideen NMP, Ben Hadda T, Almalki FA, Laarousi H, Soliman SSM, Kawsar SMA. Black seeds (Nigella sativa) for the management of dengue viral disease: POM analyses for the identification of new antiviral pharmacophore sites. Journal of Medicinal Herbs and Ethnomedicine. 2023; 14(1): 19-36.
37. Sana T, Khan M, Jabeen A, Shams S, Ben Hadda T, Begum S, Siddiqui BS. Urease and carbonic anhydrase inhibitory effect of xanthones from aspergillus nidulans, an endophytic fungus of nyctanthes arbor-tristis. Planta Medica. 2023; 89(04): 377-84. doi: 10.1055/a-1908-0935
38. Rbaa M, Haida S, Tuzun B, Hichar A, El Hassan A, Kribii A, Lakhrissi Y, Ben Hadda T, Zarrouk A, Lakhrissi B, Berdimurodov E. Synthesis, characterization and bioactivity of novel 8-hydroxyquinoline derivatives: experimental, molecular docking, DFT and POM analyses. Journal of Molecular Structure. 2022; 1258: 132688. doi: 10.1016/j.molstruc.2022.132688
39. Kawsar SMA, Almalki FA, Ben Hadda T, Laaroussi H, Khan MAR, Hosen MA, Mahmud S, Aounti A, Maideen NMP, Heidarizadeh F, Soliman SSM. Potential antifungal activity of novel carbohydrate derivatives validated by POM, molecular docking and molecular dynamic simulations analyses. Molecular Simulation. 2023; 49(1): 60-75. doi: 10.1080/08927022.2022.2123948
40. Akkoc S, Karatas H, Muhammed MT, Kökbudak Z, Ceylan A, Almalki F, Laaroussi H, Ben Hadda T. Drug design of new therapeutic agents: molecular docking, molecular dynamics simulation, DFT and POM analyses of new Schiff base ligands and impact of substituents on bioactivity of their potential antifungal pharmacophore site. Journal of Biomolecular Structure and Dynamics. 2023; 41(14): 6695-708. doi: 10.1080/07391102.2022.2111360
41. Kote R B, Bankar A A, Argade V P, Kadam A J. Qualitative Assessment of Achyranthes aspera Linn bark extract. Asian Journal of Pharmacy and Technology. 2024; 14(2): 103-7.
42. Kavitha Sagar, Soma Aneesha, Pooja Uppin, Gowthami. Phytochemical Studies and Quantification of total content of Phenols, Tannins and Flavonoids in selected endangered plant species. Res. J. Pharmacognosy and Phytochem. 2018; 10(4): 277-281.
43. Rupak K. Swain, Satya N. Tripathy, Rabinarayan Rana, Sujit K. Patro, Satyajeet Behera. A Study on Phytochemical and Pharmacological activity of Terminalia arjuna: A Review. Research Journal of Pharmacognosy and Phytochemistry. 2023; 15(2): 111-7.
44. Durga , D. Sheela. Biomolecules Detection among Selected Spilanthes Jacq. species from Kerala, India. Res. J. Pharmacognosy and Phytochem. 2018; 10(1): 36-38.
45. Thube Smita, Shaikh Gulam, M. Sifarish, Baig R. Samrana. In-Vitro Anthelmintic Activity of Root Bark of Tabernaemontana citrifolia Linn against Intestinal Helminthiasis. Research J. Pharm. and Tech. 2011; 4(12): Dec. 1912-1914.
46. B.S.Krishnamoorthy, N. Nattuthurai, Arunkumar K., Revathi T., S. Kamatchi. Preliminary Studies on Phytochemicals of Solvent Extracts of Eichhornia crassipes. Research J. Pharm. and Tech. 2014; 7(6): June, 635-637.
47. Natalya P. Kobzar, Irina A. Sych, Lina O. Perekhoda. Search for Biologically Active Substances with Antimicrobial and Antifungal Action in the series of 2.5-disubstituted 1, 3, 4-tiadiazoles. Research J. Pharm. and Tech. 2019; 12(6): 2871 – 2876.
48. J. Angel Steffy, M. Henna Parveen, V. Durga , S. Manibalan. Extraction Purification of Phlorotannins from Different Species of Marine Algae and Evaluation of their Anti-Oxidant Potential. Research J. Engineering and Tech. 2013; 4(4): Oct.-Dec., 163-168.