Author(s):
Swapnashree Satapathy, Asit Ray, Dattatreya Kar, Anindya Bose, Ananya Kuanar
Email(s):
ananyakuanar@gmail.com
DOI:
10.52711/0974-360X.2025.00244 
Address:
Swapnashree Satapathy1, Asit Ray1, Dattatreya Kar2, Anindya Bose3, Ananya Kuanar1*
1Centre for Biotechnology, Siksha ‘O’ Anusandhan (Deemed to be University), Kalinga Nagar, Ghatikia, Bhubaneswar - 751003, Odisha, India.
2Department of Medical Research, Health Science, IMS and SUM Hospital, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar - 751003, Odisha, India.
3Faculty of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Kalinga Nagar, Ghatikia, Bhubaneswar - 751003, Odisha, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 4,
Year - 2025
ABSTRACT:
Ginger, scientifically identified as Zingiber officinale Rosc, is a rhizome belonging to a plant that grows underground; it’s popularly utilized as a spice in the food and beverage sectors. Gingerols have various beneficial effects on health, such as reducing inflammation, antioxidant, preventing cancer, relieving pain, protecting the stomach, strengthening the heart, reducing fever, inhibiting blood vessel growth, preventing blood clotting and lowering blood sugar levels. High-performance liquid chromatography (HPLC) can quickly and easily separate, identify and measure the compounds found in ginger rhizome. The rhizomes of Z. officinale Rosc. were found to contain four gingerols using a fast and reliable HPLC method combined with a diode array detector (HPLC-DAD). The four compounds [6], [8], [10]-gingerol, and [6]-shogaol were separated on an Agilent TC (2) C18 column (250 × 4.6 mm, 5 µm) using a mixture of acetonitrile and HPLC water (40:60) at 40°C. The detection wavelength was set to 272 nm, with a flow rate of 1.0 mL/min. Good linear calibration curves (R² = 1) were used to measure each component. This method was successfully used to quantify the four gingerols in Zingiber officinale Rosc. from various locations in Odisha to evaluate their quality.
Cite this article:
Swapnashree Satapathy, Asit Ray, Dattatreya Kar, Anindya Bose, Ananya Kuanar. Detection and Estimation of Gingerol and its derivatives in Zingiber officinale Rhizome collected from different regions of Eastern India. Research Journal of Pharmacy and Technology. 2025;18(4):1702-8. doi: 10.52711/0974-360X.2025.00244
Cite(Electronic):
Swapnashree Satapathy, Asit Ray, Dattatreya Kar, Anindya Bose, Ananya Kuanar. Detection and Estimation of Gingerol and its derivatives in Zingiber officinale Rhizome collected from different regions of Eastern India. Research Journal of Pharmacy and Technology. 2025;18(4):1702-8. doi: 10.52711/0974-360X.2025.00244 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-4-35
REFERENCES:
1. Arawande JO, Akinnusotu A, Alademeyin JO. Extractive value and phytochemical screening of ginger (Zingiber officinale Roscoe) and turmeric (Curcuma longa) using different solvents. Journal of Natural Medicines. 2018; 8(1): 13–22
2. Aly UI, Abbas MS, Tahal HS, Gaber EI. Characterization of 6-gingerol for in vivo and in vitro ginger (Zingiber officinale) using high performance liquid chromatography. Global Journal of Botanical Science. 2013; 1: 9–17
3. Rajurkar RM, Jain RG, Bedmohta PA, Khadbadi SS. Antioxidant activity of phenolic extract from ginger (Zingiber officinale Roscoe) rhizome. Asian Journal of research in Chemistry. 2009;2(3):260-1
4. Pattnaik P K, Kar D, Kuanar A, Mishra B. Screening of Ginger Germplasm for Resistance to Rhizome Rot. Proceedings of the National Academy of Sciences India Section B Biological Sciences. 2015; 85: 303-308
5. Peat JK, Li J. Reversing the trend: reducing the prevalence of asthma. Journal of Allergy Clinical Immunology. 1999; 96: 111-6
6. Kar D, Pattanaik PK, Acharya L, Panda MK, Sathapathy K, Kuanar A, Mishra B. Assessment of genetic diversity among some elite cultivars of ginger (Zingiber officinale Rosc.) using isozyme and protein markers. Brazilian Journal of Botany 2014; 37:469-479
7. Pattnaik PK, Kar D, Kuanar A, Sahu A, Mishra B. Molecular and Biochemical characterization of selected elite accessions of ginger. Biocatalysis and Agricultural Biotechnology. 2016; 8: 130-138
8. Wang Y, Wei X, Wang F, Xu J, Tang X, Li N. Structural characterization and antioxidant activity of polysaccharide from ginger. International Journal of Biological Macromolecules. 2018; 111: 862-869
9. Ansari MJ, Ahmad S, Kohli K, Ali J, Khar RK. Stability–indicating HPTLC determination of curcumin in bulk drugs and pharmaceutical formulations. Journal of Pharmaceutical and Biomedical Analysis. 2005; 39: 132–138
10. Areer AlNW, Azzam Al KM, Omari Al RH, Deeb AlI, Bekbayeva L, Negim ES. Quantitative analysis of total phenolic and flavonoid compounds in different extracts from ginger plant (Zingiber officinale Roscoe) and evaluation of their anticancer effect against colorectal cancer cell lines. Pharmacia. 2023; 70(4): 905-919
11. Balladin DA, Headley O, Yen C, Mcgaw DR. High pressure liquid chromatographic analysis of the main pungent principles of solar dried West Indian ginger (Zingiber officinale Roscoe). Renew Energy. 1998; 13(4): 531-36.http://doi.org/10.1016/S0960-1481-98-00029-9
12. Rout S, Dutta S, Rath B. Evaluation of Broncho-Protective Effect of Zingiber officinale Roscoe in Histamine Induced Broncho-Spasm. Research Journal of Pharmacy and Technology. 2010; 3(2): 589-91
13. Ishaq BM, Rajan VS, Parameswari SA, Amruth N, Madhu M, Chetty CM. Analytical Method Development and Validation of Erlotinib by High Performance Liquid Chromatography. Research Journal of Pharmacy and Technology. 2011; 4(11): 1787-90
14. Ragab G. Elshahaly M. Bardin T. Gout: An old disease in new perspective – A review. J Adv Res. 2017; 8(5): 495-511. doi:10.1016/j.jare.2017.04.008
15. Gandhimathi M. et al. RP-HPLC determination of Simvastatin and Nicotinic acid in tablets. Journal of Indian drug 2003; 40; 12: 707-711
16. Mohammed KA, Wasfy AA, Bazalou MS. Qualitative analysis of ethanolic extract of ginger (Zingiber officinale Rosc) by gas chromatography triple quad time-flight (GC-Q-TOF) technology. Research Journal of Pharmacy and Technology. 2021; 14(8): 4307-13
17. Widyaningsih TD, Sari RA, Widya RD, Dahniar SA. The effect of Moringa leaf (Moringa oleifera Lam.), Pandan Wangi (Pandanus amaryllifolius Roxb.) and red ginger (Zingiber officinale rosc. var) extract supplement intervention on uric acid, kidney, liver function and perceptions of hyperuricemia patients on health benefit. Research Journal of Pharmacy and Technology. 2022; 15(10): 4477-84
18. Patel K, Patel D. Simultaneous method development and Validation by HPLC for Capecitabine and Oxaliplatin in mucoadhesive microspheres containing capsules. Research Journal of Pharmacy and Technology. 2021; 14(6): 3365-70
19. Govindarajan VS, Connell DW. Ginger—chemistry, technology, and quality evaluation. Critical Reviews in Food Science and Nutrition. 1983; 17(1):1-96
20. Zick SM, Ruffin MT, Djuric Z, Normolle D, Brenner DE. Quantitation of 6-, 8-and 10-gingerols and 6-shogaol in human plasma by high-performance liquid chromatography with electrochemical detection. International journal of Biomedical Science. 2010; 6(3): 233
21. Zick SM, Ruffin MT, Djuric Z, Normolle DP. Pharmacokinetics of 6-gingerol, 8-gingerol, 10-gingerol, and 6-shogaol and conjugate metabolites in healthy human subjects. 2008
22. Sharma S, Singh N, Ankalgi AD, Rana A, Ashawat MS. A laconic review on liquid chromatography mass spectrometry (LC-MS) based proteomics technology in drug discovery. Asian Journal of Pharmaceutical Research. 2021; 11(3): 194-201. dx.doi.org/10.52711/2231-5691.2021.00035
23. Hasan HA, Raauf AMR, Razik BMA, Hassan BAR. Chemical composition and antimicrobial activity of the crude extracts isolated from Zingiber Officinale by different solvents. Pharmaceutica Analytica Acta. 2012; 3(9): 1–5
24. Jiang H, Xie Z, Koo HJ, McLaughlin SP, Timmermann BN, Gang DR. Metabolic profiling and phylogenetic analysis of medicinal zingiber species: tools for authentication of ginger (Zingiber officinale Rosc.). Phytochemistry. 2016; 67: 1673–85
25. Jolad SD, Lantz RC, Chen GJ, Bates RB, Timmermann BN. Commercially processed dry ginger (Zingiber officinale): composition and effects on LPS-stimulated PGE2 production. Phytochemistry. 2005; 66:1614–1635
26. Jolad SD, Lantz RC, Solyom AM, Chen GJ, Bates RB, Timmermann BN. Fresh organically grown ginger (Zingiber officinale): composition and effects on LPS-induced PGE2 production. Phytochemistry. 2004; 65: 1937-54
27. Kajsongkram T, Rotamporn S, Limbunruang S, Sirinan T. Development and validation of a HPLC method for 6-gingerol and 6-shogaol in joint pain relief gel containing ginger (Zingiber officinale).International Journal of Medical, Health, Pharmaceutical and Biomedical Engineering. 2015; 9: 805
28. Pandey NK, Singh SK, Ghosh D, Khursheed R, Kumar R, Kapoor B, Kumar B, Awasthi A. Method development and validation for simultaneous estimation of glimepiride and simvastatin by using reversed phase high-performance liquid chromatography. Research Journal of Pharmacy and Technology. 2020; 13(4): 1655-9
29. Kumar A, Singh SK, Gulati M, Kumar B, Prasher R, Gupta J, Mankotia D, Pandey NK, Singh S. Method development and Validation for the Estimation of 5-Fluorouracil by using Reverse phase high-performance liquid chromatography. Research Journal of Pharmacy and Technology. 2021; 14(1): 185-8
30. Vijayalakshmi R, Anbazhagan S. Development and Validation of Stability Indicating Method for Low Content of Fenpiverinium Bromide Using a Supelcosil LC-CN Column By RP-HPLC. Research Journal of Pharmacy and Technology. 2011; 4(11): 1777-81
31. Ngamkhae N, Chulikhit Y, Monthakantirat O, Maneenet J, Khamphukdee C, Boonyarat C, Daodee S. Development and validation of a high-performance liquid chromatography Method for simultaneous determination of five active compounds in Kleeb Bua Daeng formula. Research Journal of Pharmacy and Technology. 2022; 15(8): 3618-26
32. Youseff R, Alassaf Z. Simultaneous Identification and Quantification of Phenylalanine, Glycine and Lysine amino acids and conjugated heterocyclic amines in Maillard chemical model system using RP-HPLC-DAD. Research Journal of Pharmacy and Technology. 2024; Feb 20; 17(2): 538-46
33. Lee S, Khoo C, Halstead CW, Huynh T, Bensoussan A. Liquid chromatographic determination of 6-, 8-, 10-gingerol, and 6-shogaol in ginger (Zingiber officinale) as the raw herb and dried aqueous extract. The Journal of AOAC International. 2007; 90(5): 1219-1226
34. Lawrence BM. Progress in essential oils. Perfumer and Flavorist. 2001; 26(1): 36-51
35. Pawar N, Pai S, Nimbalkar M, Dixit G. RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from different ginger cultivars. Food Chemistry. 2011; 126(3): 1330–6
36. Prasad S, Tyagi AK. Ginger and its constituents: role in the prevention and treatment of gastrointestinal cancer. Gastroenterology Research and Practice. 2015; 142979
37. Hemdan A, Eissa MS. Simultaneous chromatographic analysis of sofosbuvir/ledipasvir in their combined dosage form: an application to green analytical chemistry. Journal of Anal Science Technology. 2019); 10(39): 1-12
38. Mohamed N. Simultaneous Determination of Amlodipine and Valsartan. Analytical Chemistry Insights. 2011; 6: 53–59
39. Rafi M, Wahim I, Takeuchi T, Usman LKD. Simultaneous determination of gingerols and shogaol using capillary liquid chromatography and its application in discrimination of three ginger varieties from Indonesia. Talanta. 2013 103: 28–32
40. Sanwal SK, Rai N, Singh J, Buragohain J. Antioxidant phytochemicals and gingerol content in diploid and tetraploid clones of ginger (Zingiber officinale Roscoe). Scientia Horticulturae. 2010; 124: 280–5
41. Sasidharan I, Menon AN. Comparative chemical composition and antimicrobial activity fresh and dry ginger oils (Zingiber Officinale Roscoe). International Journal of Current Pharmaceutical Research. 2010; 4(2): 92–8
42. Silva JA, Becceneri AB, Muttib HS, Martinc ACBM, Gra MF, DaSilv F, Fernandesa JB, Vieiraa PC, Cominettib MR. Purification and differential biological effects of ginger-derived substances on normal and tumor cell lines. Journal of Chromatography A. 2012; 903: 157–62
43. Wagner H, Bladt S. Plant Drug Analysis: A Thin Layer Chromatography Atlas, Springer, New York, NY. 1996
44. Wohlmuth H, Leach DN, Smith MK, Myers SP. Gingerol content of diploid and tetraploid clones of ginger (Zingiber officinale Roscoe). Journal of Agricultural and Food Chemistry. 2011 62(6):329–43
45. Zhang M, Viennois E, Prasad M, Zhang Y, Wang L, Zhang Z. Edible ginger-derived nanoparticles: a novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer. Biomaterial. 2016; 101: 321-40
46. Mishra V, Thakur S, Patil A, Shukla A. Quality by design (QbD) approaches in current pharmaceutical set-up. Expert Opinion on Drug Delivery. 2018; 15 (8): 737-758. doi: 10.1080/17425247.2018.1504768
47. Sharma PK, Singh V and Ali M. Chemical composition and antimicrobial activity of fresh rhizome essential oil of zingiber officinale roscoe. Journal of Pharmacognosy. 2016; 8(3)
48. Anil DM, Nandini RP. Simultaneous Determination of Eight Phytoconstituents in Triphala churna by HPLC–DAD. Research Journal of Pharmacognosy and Phytochemistry. 2011; 3(2): 62-66
49. Hsiao HY, Chen BH, Kao TH. Analysis of heterocyclic amines in meat by the quick, easy, cheap, effective, rugged, and safe method coupled with LC-DAD-MS-MS. Journal of Agricultural and Food Chemistry. 2017; 65(42): 9360-8. doi.org/10.1021/acs.jafc.7b03739