Author(s):
Hemavathi N, Pinki Verma, Spoorthi N
Email(s):
vermapinki05@gmail.com
DOI:
10.52711/0974-360X.2025.00222 
Address:
Hemavathi N, Pinki Verma*, Spoorthi N
Department of Pharmacology, Aditya Bangalore Institute of Pharmacy Education and Research, Rajiv Gandhi University of Health Sciences, Bangalore - 560064, Karnataka, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 4,
Year - 2025
ABSTRACT:
Objective: To investigate the antidepressant and antioxidant activity of ethanolic extract of Evolvulus alsinoides leaves and its effect on TNF-a expression in mice hippocampus. Methods: The extraction of collected leaves was carried out by Soxhlet apparatus using 98% of ethanol. Phytochemical screening of the extract was carried out by using standard procedure. Antidepressant activity of the extract was evaluated by using the forced swim test and tail suspension test. Locomotor activity was investigated by using actophotometer and open field test. Antioxidant activity was evaluated by DPPH Assay. Further Immunohistochemistry (TNF – a expression) was evaluated in mice hippocampus. The liver and brain were dissected, weighed, and processed for histopathological analysis. Results and Discussions: In present study, Phytochemical screening of extract proved the presence of alkaloids, flavonoids, phenols, proteins, carbohydrates, glycosides, saponins and steroids. Oral administration of extracts for 21 days showed significant (P< 0.01) decrease in immobility in the tail suspension and forced swim models of depression without any stimulant effect of the extract. The antioxidant activity was determined by % of radical scavenging and IC50 value of extract was found to be 2.47 µg/ml. In comparison to the control group, extract at 400 mg/kg was able to lower the expression of TNF-alpha. Histopathology of brain showed recovery of cells in E.alsinoides (400 mg/kg) treated mice. From liver histopathology it was confirmed that the extract was free from toxicity. Conclusion: The results showed that extract exhibits antidepressant, antioxidant and lowering TNF-a expression in Swiss albino mice.
Cite this article:
Hemavathi N, Pinki Verma, Spoorthi N. Antidepressant and Antioxidant Activity of Ethanolic Extract of Evolvulus alsinoides Leaves and its effect on TNF-α Expression in Mice Hippocampus. Research Journal of Pharmacy and Technology. 2025;18(4):1551-6. doi: 10.52711/0974-360X.2025.00222
Cite(Electronic):
Hemavathi N, Pinki Verma, Spoorthi N. Antidepressant and Antioxidant Activity of Ethanolic Extract of Evolvulus alsinoides Leaves and its effect on TNF-α Expression in Mice Hippocampus. Research Journal of Pharmacy and Technology. 2025;18(4):1551-6. doi: 10.52711/0974-360X.2025.00222 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-4-13
REFERENCES:
1. Praveen Kumar Uppala, Swarna Latha M, Shashidhar Reddy R, G Chakravarthi. Evaluation of anti-depressant activity of Methanolic Seed Extract of Avena sativa L. In Mice. Research J. Pharmacology and Pharmacodynamics. 2013; 5(4). 212-217.
2. Rifa’atul Mahmudah, Silviana Hasanuddin, Ahmad Saleh, Wa Ode Yuliastri, Muhammad isrul. Antidepressant Activity and Identification of Chemical Compounds Extract Mustard Leaves (Brassica juncea L.). Research J. Pharm. and Tech. 2019; 12(7): 3223-3227. https://doi.org/10.5958/0974-360X.2019.00542.0
3. Qun XU, Ying PAN, Li-Tao YI, Yu-Cheng LI, Shi-Fu MO, Fu-Xin JIANG, Chun-Feng QIAO, Hong-Xi XU, Xiao-Bo LU, Ling-Dong KONG,Hsiang-Fu KUNG.Antidepressant-like effects of psoralen isolated from the seeds of Psoralea corylifolia in the mouse forced swimming test. Biol Pharm Bull. 2008; 31 (6): 1109-1114. https://doi.org/10.1016/j.pnpbp.2007.10.005
4. G. Gopi, A. Elumalai, P. Jayasri. In-Vitro Antioxidant Activity of Kedrostis foetidissima (Jacq) Cogn . Research J. Pharmacognosy and Phytochemistry. 2012; 4(4): 209-211.
5. Austuin DF. Evolvulus alsinoides (Convolvulaceae): An American herb in the Old World. J Ethnopharmacol. 2008; 117(2): 185-198. https://doi.org/10.1016/j.jep.2008.01.038
6. Pavithra Sivaraja and Kannan Kilavan Packiam. A Review on pharmacognosy and pharmacology of Evolvulus alsinoides. Int. Res. J. Pharm. 2017; 8 (7): 1-4. http://dx.doi.org/10.7897/2230-8407.087110.
7. Yadav MK, Singh SK, Singh M, Mishra SS, Singh AK, Tripathi JS, Tripathi YB. In Vivo Toxicity Study of Ethanolic Extracts of Evolvulus alsinoides and Centella asiatica in Swiss Albino Mice. Open Access Macedonian Journal of Medicinal Sciences. 2019; 7(7): 1071-1076. https://doi.org/10.3889/oamjms.2019.209
8. Nizamudeen. T, Ramanjaneyulu. J, Veeresh Babu. D, Narayana Swamy V.B. A Study on Antidepressant Activity of Medha gulika-a Polyherbal Formulation in Experimental Animal Models. Asian J. Pharm. Tech. 2015; 5(2): 115-121. https://doi.org/10.5958/2231-5713.2015.00017.3.
9. Rahul Jodh, Mukund Tawar, Aparna Kachewar. Evaluation of Antidepressant activity of Tricholepis glaberrima DC using various Paradigms. Research Journal of Pharmacy and Technology. 2022; 15(12): 5610-5616. https://doi.org/10.52711/0974-360X.2022.00947.
10. Valli G, Vasanthi A, Vijayalakshmi R, Thanga Thirupathi A. CNS Depressant and Analgesic Activities of Different Extracts of Tephrosia purpurea Root. Research J. Pharm. and Tech. 2011; 4(9): 1439-1442. AbstractView.aspx?PID=2011-4-9-13.
11. Gururaja MP, Nidhi P Shetty, Himanshu Joshi. Attenuation of Experimentally Induced Anxiety by Panchagavya Ghrita in Mice. Research Journal of Pharmacy and Technology. 2022; 15(11): 4897-4900. https://doi.org/10.52711/0974-360X.2022.00822.
12. Felipe FCB, Filho JTS, Souza LEO, Silveira JA, Uchoa DEA, Silveira ER, Piplartine, an amide alkaloid from Piper tuberculatum, presents anxiolytic and antidepressant effects in mice. Phytomedicine. 2007; 14(9): 605–612. https://doi.org/10.1016/j.phymed.2006.12.015.
13. Mrunali Potbhare, Deepak Khobragade. In Vitro Evaluation of Antioxidant Potential of Ayurvedic Preparations Lauha Bhasma and Mandura Bhasma. Asian J. Pharm. Res. 2017; 7(2): 63-66. https://doi.org/10.5958/2231-5691.2017.00011.9
14. Parisa N, Hidayat R, Maritska Z, Prananjaya BA. Antidepressant Effect of Cinnamon (Cinnamomum burmannii) Bark Extract in Chronic Stress-Induced Rats. Open Access Maced J Med Sci. 2020; 8(A): 273-277. https://doi.org/10.3889/oamjms.2020.3995.
15. Sudha Bansal, Dinesh Dhingra. Behavioral, Biochemical and Histopathological Evaluation of Sinapic acid for Antidepressant activity in Normal Mice and Stressed Mice. Research Journal of Pharmacy and Technology. 2023; 16(8): 3569-3577. https://doi.org/10.52711/0974-360X.2023.00589.
16. Priyanka B, Sridhar Y, Shankaraiah P . Antidepressant and muscle relaxant activity of Cardiospermum halicacabum Linn. Roots in mice. J. Advanced Pharmaceutical Sciences. 2012; 2(1): 193–200. https://pmc.ncbi.nlm.nih.gov/articles/PMC4202599.
17. Potdar VH, Kibile SJ. Evaluation of Antidepressant-like Effect of Citrus maxima Leaves in Animal Models of Depression. Iran J Basic Med Sci. 2011; 14(5): 478-483. https://pmc.ncbi.nlm.nih.gov/articles/PMC3586848.
18. Muhammad Aslam.Tail suspension test to evaluate the antidepressant activity of experimental drugs. Bangladesh Journal of Pharmacology. 2016; 11(2): 292-294. https://doi.org/10.3329/bjp.v11i2.26517.
19. G. Venkatesha, C. Kalaiyarasia, M. Ramanathana. Antidepressant like Effect of Gabapentin Decreases the Immobility Time in Despair Animal Models in Mice: Role of Serotonergic System in it. Research J. Pharm. and Tech. 2011; 4(11): 1702-1706. https://doi.org/10.53730/ijhs.v6nS1.6721.
20. Lee SC, Wang SY, Li CC, Liu CT. Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum kanehira in endotoxin-induced mice. J Food Drug Anal. 2018; 26(1): 211-220. https://doi.org/10.1016/j.jfda.2017.03.006
21. Zhu CB, Lindler KM, Owens AW, Daws LC, Blakely RD, Hewlett WA. Interleukin-1 receptor activation by systemic lipopolysaccharide induces behavioral despair linked to MAPK regulation of CNS serotonin transporters. Neuropsychopharmacology. 2010; 35(10): 2510–2520. https://doi.org/10.1038/npp.2010.116.