Author(s):
U.S. Mahadeva Rao, S. Ragunandhakumar, G. Shoba, D. Saravanan, P. Malliga, G. Sriram Prasath, Kasim Sakran Abass
Email(s):
sriram@dgvaishnavcollege.edu.in
DOI:
10.52711/0974-360X.2025.00198 
Address:
U.S. Mahadeva Rao1, S. Ragunandhakumar2, G. Shoba3, D. Saravanan4, P. Malliga5 , G. Sriram Prasath6*, Kasim Sakran Abass7
1Professor, School of Basic Medical Sciences, Faculty of Medicine, Kampus Perubatan, Universiti Sultan Zainal Abdin 20400 Kuala Terengganu Malaysia.
2Associate Professor, Cancer and Stem Cell Research Lab, Department of Pharmacology, Saveetha Dental College, Chennai-600 077.
3 Post Graduate Department of Biotechnology, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam, Chennai - 106, Tamilnadu. India.
4Associate Professor and Head, Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam, Chennai-106, Tamilnadu. India.
5Post Graduate Student, PG and Research Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam, Chennai-106 Tamilnadu India.
6Assistant Professor, Department of Biochemistry, Dwaraka Doss Goverdhan Doss Vaishnav College, Arumbakkam, Chennai-106, Tamilnadu. India.
7Departmen
Published In:
Volume - 18,
Issue - 3,
Year - 2025
ABSTRACT:
Osteosarcoma is a type of bone cancer that usually develops in the osteoblast cells, which are responsible for making new bone tissue. It is a high-grade primary skeletal cancer caused by spindle cells of mesenchymal origin that deposit immature osteoid matrix. Phytochemicals and their derivatives are promising options to improve treatment efficiency in cancer patients and decrease adverse reactions. ß-sitosterol is a prominent dietary phytosterol found in beans, nuts, and seeds known for its significant pharmacological properties. In the present study and attempt has been made to evaluate the anticancer properties of ß sitosterol in MG 63 Osteosarcoma cell line. The MTT assay revealed a significant decrease in cell viability of MG-63 human osteosarcoma cells upon treatment with ß-sitosterol in a dose-dependent manner. This reduction in cell viability indicates the potential of ß-sitosterol to exert an anticancer effect against osteosarcoma cells. There was dose dependent decrease in percentage of cell viability. The cell viability was around 20% in 320 µg/ml indicating the fact that ß-sitosterol can act as cytotoxic drug against cancer cells. Treatment with the ß sitosterol indicated a marked inhibition in cell viability indicating its antiproliferative nature. DAPI staining revealed changes in nuclear morphology, such as condensation or fragmentation, which are characteristic of apoptotic cells. The result obtained in Docking analysis of ß sitosterol and Bcl-2 indicate that the Binding Energy for beta sitosterol and Bcl-2 shows -7.7 kcal/mol and forms four hydrophobic interactions with ARG107, LEU201 and PHE104. Therefore, ß sitosterol acts as BCL2 inhibitors which holds great promise for the use of the compound in treating cancer.
Cite this article:
U.S. Mahadeva Rao, S. Ragunandhakumar, G. Shoba, D. Saravanan, P. Malliga , G. Sriram Prasath, Kasim Sakran Abass. Anticancer property of β-sitosterol studied in MG 63 Osteosarcoma cell line. Research Journal of Pharmacy and Technology. 2025;18(3):1374-9. doi: 10.52711/0974-360X.2025.00198
Cite(Electronic):
U.S. Mahadeva Rao, S. Ragunandhakumar, G. Shoba, D. Saravanan, P. Malliga , G. Sriram Prasath, Kasim Sakran Abass. Anticancer property of β-sitosterol studied in MG 63 Osteosarcoma cell line. Research Journal of Pharmacy and Technology. 2025;18(3):1374-9. doi: 10.52711/0974-360X.2025.00198 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-3-59
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