Author(s):
Atul Baravkar, Vitthal Chopde, Pradeep Bodake, Amit Lunkad, Sonali Pawar, Aditi Kushare, Bindurani LGP Ram, Dattatray Kature, Avinash Tupe, Gaffar Sayyad, Sanjay Garje
Email(s):
atul200678@gmail.com
DOI:
10.52711/0974-360X.2025.00196 
Address:
Atul Baravkar1*, Vitthal Chopde2, Pradeep Bodake3, Amit Lunkad4, Sonali Pawar5, Aditi Kushare6, Bindurani LGP Ram7, Dattatray Kature8, Avinash Tupe9, Gaffar Sayyad10, Sanjay Garje10
1Agricultural Development Trust’s School of Pharmacy and Research Centre, Baramati, India.
2Modern College of Pharmacy, Pune, India.
3S. B. Patil College of Pharmacy, Indapur, India.
4Sitabai Thite College of Pharmacy, Shirur, India.
5Vishal Institute of Pharmaceutical Education and Research, Junnar, India.
6Mahatma Gandhi Vidyamandir’s College of Pharmacy, Nashik, India.
7Dnyanvilas College of Pharmacy, Pune, India.
8Delonix Society’s Baramati College of Pharmacy, Pune, India.
9SVPM’s College of Pharmacy, Baramati, India.
10SAJVPMs College of Pharmaceutical Sciences and Research Center, Beed, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 3,
Year - 2025
ABSTRACT:
The demand for natural surfactants has surged due to concerns regarding synthetic surfactants' impact on human health and the environment. Sapindus mukorossi, or soapnut, has emerged as a source of natural surfactants. This review focuses on the extraction, isolation, and characterization of these saponins. While the phytochemical composition of Sapindus mukorossi, has been extensively studied, challenges remain in their extraction and isolation due to structural complexity and labile functionalities. Cold extractions with ethanol-water solutions are preferred for obtaining the real saponin composition. Various separation techniques like thin-layer chromatography, column chromatography, and high-performance liquid chromatography are employed, with flash chromatography emerging as a rapid and cost-effective method. The isolated saponins are further characterized using UV spectroscopy, Fourier-transform infrared spectroscopy, mass spectrometry, nuclear magnetic resonance spectroscopy, and high-performance thin-layer chromatography. In conclusion, Sapindus mukorossi particularly saponins, with potential applications. Further research is necessary to optimize extraction methods and explore their pharmacological potential.
Cite this article:
Atul Baravkar, Vitthal Chopde, Pradeep Bodake, Amit Lunkad, Sonali Pawar, Aditi Kushare, Bindurani LGP Ram, Dattatray Kature, Avinash Tupe, Gaffar Sayyad, Sanjay Garje. Development of Novel Method for Isolation of Herbal Surfactant from selected Medicinal Plants by Flash Chromatography and Characterization by HPTLC, IR and PMR.. Research Journal of Pharmacy and Technology. 2025;18(3):1357-7. doi: 10.52711/0974-360X.2025.00196
Cite(Electronic):
Atul Baravkar, Vitthal Chopde, Pradeep Bodake, Amit Lunkad, Sonali Pawar, Aditi Kushare, Bindurani LGP Ram, Dattatray Kature, Avinash Tupe, Gaffar Sayyad, Sanjay Garje. Development of Novel Method for Isolation of Herbal Surfactant from selected Medicinal Plants by Flash Chromatography and Characterization by HPTLC, IR and PMR.. Research Journal of Pharmacy and Technology. 2025;18(3):1357-7. doi: 10.52711/0974-360X.2025.00196 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-3-57
5. REFERENCES:
1. Astalakshmi N. Isolation, Characterization, Elucidation of Isolated Phyto Constituents and Screening of Anti-Microbial and Anti-Oxidant Activity of Delonix regia (BOJ. Ex. HOOK) RAF Leaves. Doctoral dissertation, Madurai Medical College, Madurai. 2008.
2. Chen Y, Xie Y, Li L, Wang Z, Yang L. Advances in mass spectrometry imaging for toxicological analysis and safety evaluation of pharmaceuticals. Mass Spectrometry Reviews. 2023; Sep 1; 42(5): 2207-33.
3. Suhagia BN, Rathod IS, Sindhu S. Sapindus mukorossi (Areetha): an overview. International Journal of Pharmaceutical Sciences and Research. 2011; Aug 1; 2(8): 1905.
4. Sharma A, Sati SC, Sati OP, Sati MD, Kothiyal SK, Semwal DK, Mehta A. A new triterpenoid saponin and antimicrobial activity of ethanolic extract from Sapindus mukorossi Gaertn. Journal of Chemistry, 2013.
5. Hu Q, Chen YY, Jiao QY, Khan A, Li F, Han DF, Cao GD, Lou HX. Triterpenoid saponins from the pulp of Sapindus mukorossi and their antifungal activities. Phytochemistry. 2018 Aug 1;147:1-8.
6. Sengupta A, Basu SP. Chemical investigations of the Sapindus mukorossi seed oil. Fette, Seifen, Anstrichmittel. 1982; Oct 1; 84(10): 411-5.
7. Chen C, Li R, Li D, Shen F, Xiao G, Zhou J. Extraction and purification of saponins from Sapindus mukorossi. New Journal of Chemistry. 2021; Feb 1; 45(2): 952-60.
8. Chen CC, Nien CJ, Chen LG, Huang KY, Chang WJ, Huang HM. Effects of Sapindus mukorossi seed oil on skin wound healing: In vivo and in vitro testing. International Journal of Molecular Sciences. 2019; Oct 1; 20(10): 2579.
9. Wei MP, Zhu XW, Yu H, Xie YF, Guo YH, Cheng YL, Qian H, Yao WR. Isolation of two sesquiterpene glycosides from Sapindus mukorossi Gaertn. with cytotoxic properties and analysis of their mechanism based on network pharmacology. Natural Product Research. 2021; Nov 1; 35(22): 4323-30.
10. Huang HC, Wu MD, Tsai WJ, Liao SC, Liaw CC, Hsu LC, Wu YC, Kuo YH. Triterpenoid saponins from the fruits and galls of Sapindus mukorossi. Phytochemistry. 2008; Jul 1; 69(7): 1609-16.
11. Zhang XM, Yang DP, Xie ZY, Xue X, Zhu LP, Wang DM, Zhao ZM. A new triterpenoid saponin and an oligosaccharide isolated from the fruits of Sapindus mukorossi. Natural Product Research. 2014; Jul 1; 28(14): 1058-64.
12. Sharma A, Sati SC, Sati OP, Sati MD, Kothiyal SK, Semwal DK, Mehta A. A new triterpenoid saponin and antimicrobial activity of ethanolic extract from Sapindus mukorossi Gaertn. Research Article, 2013.
13. Li R, Wu ZL, Wang YJ, Li LL. Separation of total saponins from the pericarp of Sapindus mukorossi Gaerten. by foam fractionation. Industrial Crops and Products. 2013; Feb 1; 51: 163-70.
14. Smith Jr CR, Mikolajczak KL. Concerning lipid composition of Sapindus mukorossi seed oil. Lipids. 1975 Jun 1;10(6):363.
15. Shah MAH, Kajal D, Deka DC. Fatty acid composition of Sapindus mukorossi seed oil. Advances in Applied Science Research. 2014; Apr 1; 5(4): 43-50.
16. Heng W, Ling Z, Na W, Youzhi G, Zhen W, Zhiyong S, Deping X, Yunfei X, Weirong Y. Extraction and fermentation-based purification of saponins from Sapindus mukorossi Gaertn. Journal of Surfactants and Detergents. 2015; Nov 1; 18: 429-38.
17. Li R, Wu ZL, Wang YJ, Li LL. Separation of total saponins from the pericarp of Sapindus mukorossi Gaerten. by foam fractionation. Industrial Crops and Products. 2013; Feb 1; 51: 163-70.
18. Heng W, Ling Z, Na W, Youzhi G, Zhen W, Zhiyong S, Deping X, Yunfei X, Weirong Y. Analysis of the bioactive components of Sapindus saponins. Industrial Crops and Products. 2014; Dec 1;61: 422-9.
19. Du M, Huang S, Zhang J, Wang J, Hu L, Jiang J. Isolation of total saponins from Sapindus mukorossi Gaerth. Open Journal of Forestry. 2014; Jan 1; 4(1): 24.
20. Bajad PN, Pardeshi AB, Pagore VP. Extraction, isolation and quantification of saponin from Dodonaea viscosa JACQ. Pharma Innov J. 2019; May 1; 8(5): 41-4.
21. Srinivasarao M, Lakshminarasu M, Anjum A, Ibrahim M. Comparative study on phytochemical, antimicrobial and antioxidant activity of Sapindus mukorossi Gaertn. and Rheum emodi Wall. ex Meissn.: in vitro studies. Annals of Phytomedicine. 2015; Dec 1; 4(2): 93-7.
22. Kaur K. Pharmacognostical and preliminary phytochemical studies on the roots of Sapindus mukorossi Gaertn. [Doctoral dissertation], 2023.