Author(s):
Aristo H. Rendra, Muhamad Insanu, Sausan N. E. Pratiwi, Irda Fidrianny
Email(s):
insanu99@itb.ac.id
DOI:
10.52711/0974-360X.2025.00163 
Address:
Aristo H. Rendra, Muhamad Insanu*, Sausan N. E. Pratiwi, Irda Fidrianny
Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung 40132, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 3,
Year - 2025
ABSTRACT:
Free radicals are reactive chemical species produced inside the body or obtained from the environment, which can cause diseases such as cancer. Antioxidants are compounds that can scavenge free radicals. One of the potential plants as a natural antioxidant is longjack. This research aimed to assess the antioxidant activity of longjack's leaves, stems, and roots utilising 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cupric ion reducing capacity (CUPRAC), as well as to evaluate total phenolic content (TPC), total flavonoid content (TFC), and the correlations between TPC and TFC with DPPH and CUPRAC antioxidant activity, along with the relationship between the two methodologies. Extraction was conducted via reflux employing n-hexane, ethyl acetate, and ethanol. The antioxidant activity was assessed using DPPH and CUPRAC methods, together with total phenolic content (TPC) and total flavonoid content (TFC), using UV-visible spectrophotometry. High-performance liquid chromatography identified the marker chemical in the ethanol extract of longjack leaves. The DPPH antioxidant activity of extracts from the leaves, stems, and roots of longjack ranges from 2.03 to 39.08mg AAE/g, whereas the CUPRAC activity ranges from 10.45 to 60.12mg AAE/g. The highest TPC was given by ethyl acetate stem extract (6.81±0.21 g GAE/100 g), while the highest TFC was given by ethyl acetate root extract (4.70±0.28g QE/100 g). Phenolic compounds showed great contribution in their DPPH and CUPRAC antioxidant activities. The two methods showed linear results in determining the antioxidant activities of the extracts. The marker compound was identified in the ethanol extract.
Cite this article:
Aristo H. Rendra, Muhamad Insanu, Sausan N. E. Pratiwi, Irda Fidrianny. Antioxidative Activities and Phytochemical content of different Organs of Longjack (Eurycoma longifolia Jack). Research Journal Pharmacy and Technology. 2025;18(3):1134-9. doi: 10.52711/0974-360X.2025.00163
Cite(Electronic):
Aristo H. Rendra, Muhamad Insanu, Sausan N. E. Pratiwi, Irda Fidrianny. Antioxidative Activities and Phytochemical content of different Organs of Longjack (Eurycoma longifolia Jack). Research Journal Pharmacy and Technology. 2025;18(3):1134-9. doi: 10.52711/0974-360X.2025.00163 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-3-24
REFERENCES:
1. Irshad M, Chaudhuri PS. Oxidant-antioxidant System: Role and Significance in Human Body. Indian Journal of Experimental Biology. 2002; 40(11): 1233-1239.
2. Poprac P, Jomova K, Simunkova M, Kollar V, Rhodes CJ, Valko M. Targeting Free Radicals in Oxidative Stress-related Human Diseases. Trends in Pharmacological Sciences. 2017; 38(7): 592-607.
3. Jadhav SS, Salunkhe VR, Magdum CS. Daily Consumption of Antioxidants: Prevention of Disease is better than Cure. Asian Journal of Pharmaceutical Research. 2013; 3(1): 33-39.
4. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free Radical Scavenging Properties of Wheat Extracts. Journal of Agricultural and Food Chemistry. 2002; 50(6): 1619-1624.
5. Kumar T, Jain V. Phytochemical Screening, Phenolic, Flavonoids, Carotenoid Contents and Antioxidant Activity of Folkloric Memecylon edule Roxb. Research Journal of Pharmacy and Technology. 2016; 9(10): 1547-1551.
6. Zohra M, Fawzia A. Chemical Composition and Antioxidant Activity of Essential Oil from Smyrnium olusatrum L. Asian Journal of Research in Chemistry. 2011; 4(2): 217-220.
7. Hassan WNAW, Zulkifli RM, Ahmad, Yunus MAC. Antioxidant and Tyrosinase Inhibition Activities of Eurycoma longifolia and Swietenia macrophylla. Journal of Applied Pharmaceutical Science. 2015; 5(8): 6-10.
8. Abubakar BM, Salleh FM, Wagiran A. Chemical Composition of Eurycoma longifolia (Tongkat Ali) and the Quality Control of Its Herbal Medicinal Products. Journal of Applied Sciences. 2017; 17(7): 324-328.
9. Triawanti, Sanyoto DD, Noor MS. The Supplementation of Pasak Bumi (Eurycoma longifolia Jack) in Undernourished Rats to Increase Spatial Memory through Antioxidant Mechanism. Clinical Nutrition Experimental. 2020; 33: 49-59.
10. Celep E, Charehsaz M, Akyüz S, Acar ET, Yesilada E. Effect of In Vitro Gastrointestinal Digestion on the Bioavailability of Phenolic Components and the Antioxidant Potentials of Some Turkish Fruit Wines. Food Research International. 2015; 78: 209-215.
11. Ozyürek M, Bektaşoǧlu B, Güçlü K, Gungor N, Apak R. Simultaneous Total Antioxidant Capacity Assay of Lipophilic and Hydrophilic Antioxidants in the Same Acetone-water Solution Containing 2% Methyl-β-cyclodextrin Using the Cupric Reducing Antioxidant Capacity (CUPRAC) Method. Analytica Chimica Acta. 2008; 630: 28-39.
12. Pourmorad F, Hosseinmehr SJ, Shahabimajd N. Antioxidant Activity, Phenol and Flavonoid Contents of Some Selected Iranian Medicinal Plants. Africal Journal of Biotechnology. 2006; 5(11): 1142-1145.
13. Chang CC, Yang MH, Wen HM, Chern J. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis. 2002; 10(3): 178-182.
14. Varghese CP, Ambrose C, Jin SC, Lim YJ, Keisaban T. Antioxidant and Anti-inflammatory Activity of Eurycoma longifolia Jack, A Traditional Medicinal Plant in Malaysia. International Journal of Pharmaceutical Sciences and Nanotechnology. 2013; 5(4): 1875-1878.
15. Dilnesa A, Mekonon A, Abebe A. Phytochemical Screening and Antioxidant Activity Investigations on the Crude Extracts of Brucea antidysenterica Leaves. EPRA International Journal of Research and Development. 2016; 1(10): 1-15.
16. Said A, Tundis R, Hawas UW, El-Kousy SM, Rashed K, Menichini, Federica, Bonesi M, Huefner A, Loizzo MR, Menichini F. In Vitro Antioxidant and Antiproliferative Activities of Flavonoids from Ailanthus excelsa (Roxb.) (Simaroubaceae) Leaves. Zeitschrift fur Naturforschung. 2010; 65(3): 180-186.
17. Rangasamy P, Hansiya VS, Maheswari PU, Suman T, Geetha N. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoria ternatea L. Blue Flowered Leaves. 2019; 9(2): 67-76.
18. Dontha S. A Review on Antioxidant Methods. Asian Journal of Pharmaceutical and Clinical Research. 2016; 9(2): 14-32.
19. Prior RL, Wu X, Schaich K. Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements. Journal of Agricultural and Food Chemistry. 2005; 53(10): 4290-4302.
20. Tiwari P. Phenolics and Flavonoids and Antioxidant Potential of Balarishta Prepared by Traditional and Modern Methods. Asian Journal of Pharmaceutical Analysis. 2014; 4(1): 5-10.
21. Jain N, Goyal S, Ramawat KG. Radical Scavenging Activity and Total Phenolic Content in Selected Fruits and Vegetables. Research Journal of Pharmacy and Technology. 2012; 5(1): 121-124.
22. Wadkar KA, Magdum CS. Evaluation of Total Phenolic Content, Flavonoid Content, and Antioxidant Activity of Stem Bark of Careya arborea Roxb. Research Journal of Pharmacognosy and Phytochemistry. 2010; 2(1): 49-51.
23. Albouchi F, Hassen I, Casabianca H, Hosni K. Phytochemicals, Antioxidant, Antimicrobial and Phytotoxic Activities of Ailanthus altissima (Mill.) Swingle Leaves. South African Journal of Botany. 2013; 87: 164-174.
24. Poljuha D, Sladonja B, Šola I, Dudaš S, Bilić J, Rusak G, Motlhatlego, KE, Eloff JN. Phenolic Composition of Leaf Extracts of Ailanthus altissima (Simaroubaceae) with Antibacterial and Antifungal Activity Equivalent to Standard Antibiotics. Natural Product Communications. 2017; 12(10): 1609-1612.
25. Patel VJ, Kpatel C, Patel HU, Patel CN. Vitamins, Minerals and Carotenoids as a Antioxidants. Asian Journal of Research in Chemistry. 2010; 3(2): 255-260.
26. Amutha K, Doss DVA. In Vitro Antioxidant Activity of Ethanolic Extract of Barleria cristata L. Leaves. Research Journal of Pharmacognosy and Phytochemistry. 2009; 1(3): 209-212.
27. Abdeldjabbar M, Messaouda D, Zhour R, Cheyma B. Comparative Analysis of Total Phenolics, Flavonoid Content and Antioxidant Profile of date palm (Phoenix dactylifera L.) with Different Watering from Oued Soufin Algeria. Asian Journal of Research in Chemistry. 2020; 13(1): 28-32.
28. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant Activity Relationships of Flavonoids and Phenolic Acids. Free Radical Biology and Medicine. 1996; 20(7): 933-956.
29. Rivera-Mondragón A, Ortíz OO, Bijttebier S, Vlietinck A, Apers S, Pieters L, Caballero-George C. Selection of Chemical Markers for the Quality Control of Medicinal Plants of the Genus Cecropia. Pharmaceutical Biology. 2017; 55(1): 1500-1512.
30. Çakir A, Mavi A, Kazaz C, Yildirim A, Küfrevioǧlu OI. Antioxidant Activities of the Extracts and Components of Teucrium orientale L. var. orientale. Turkish Journal of Chemistry. 2006; 30(4): 483-494.