Author(s):
P. K. Kulkarni, Venkatesh, K Hanumanthachar Joshi, A Noorain KM
Email(s):
aayeshanoorain@gmail.com
DOI:
10.52711/0974-360X.2025.00133 
Address:
P. K. Kulkarni, Venkatesh, K Hanumanthachar Joshi, A Noorain KM*
Department of Pharmaceutics, Sarada Vilas College of Pharmacy, Krishnamurthy Puram, Mysuru, Karnataka, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Cyanocobalamin, an important form of vitamin B12, is significantly degraded when exposed to light, affecting its potency and effectiveness. In this review, we describe in detail the effectiveness of polymeric cyclodextrins as protectants for cyanocobalamin, with a particular focus on their role in enhancing photostability. This article summarizes recent advances and explains the mechanism by which polymeric cyclodextrins protect cyanocobalamin from photodegradation. We explained the structural properties of cyclodextrin, its encapsulation ability, and synergistic interaction with cyanocobalamin. Additionally, this review evaluates the different methods and analytical techniques used to assess photostability and provide insight into the practical implications for pharmaceutical and nutraceutical applications. By synthesizing current research results, this review will narrate how polymeric cyclodextrins serve as promising candidates for preserving the photosensitivity of cyanocobalamin and thereby demonstrate its sustained efficacy and efficacy in various formulations. It aims to provide a comprehensive understanding of what contributes to their applicability.
Cite this article:
P. K. Kulkarni, Venkatesh, K Hanumanthachar Joshi, A Noorain KM. Enhancing Cyanocobalamin Stability through Polymer Cyclodextrins: A Comprehensive Review on light stability. Research Journal of Pharmacy and Technology.2025;18(2):907-1. doi: 10.52711/0974-360X.2025.00133
Cite(Electronic):
P. K. Kulkarni, Venkatesh, K Hanumanthachar Joshi, A Noorain KM. Enhancing Cyanocobalamin Stability through Polymer Cyclodextrins: A Comprehensive Review on light stability. Research Journal of Pharmacy and Technology.2025;18(2):907-1. doi: 10.52711/0974-360X.2025.00133 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-65
REFERENCES:
1. Wdowik T, Gryko D. C–C Bond Forming Reactions Enabled by Vitamin B12─ Opportunities and Challenges. ACS Catalysis. 2022; May 17; 12(11): 6517-31. https://doi.org/10.1021/acscatal.2c01596.
2. Qadeer K, Arsalan A, Ahmad I, Fatima K, Anwar Z, Ahmed S, Mahmud S. Photochemical interaction of cyanocobalamin and hydroxocobalamin with cysteine. Journal of Molecular Structure. 2021; Mar 15; 1228: 129441. https://doi.org/10.1016/j.molstruc.2020.129441
3. Juzeniene A, Nizauskaite Z. Photodegradation of cobalamins in aqueous solutions and in human blood. Journal of Photochemistry and Photobiology B: Biology. 2013; May 5; 122: 7-14. https://doi.org/10.1016/j.jphotobiol.2013.03.001
4. Carneiro SB, Costa Duarte FÍ, Heimfarth L, Siqueira Quintans JD, Quintans-Júnior LJ, Veiga Júnior VF, Neves de Lima AA. Cyclodextrin–drug inclusion complexes: In vivo and in vitro approaches. International Journal of Molecular Sciences. 2019; Feb 2; 20(3): 642. https://doi.org/10.3390/ijms20030642
5. Iacovino R, Rapuano F, Caso JV, Russo A, Lavorgna M, Russo C, Isidori M, Russo L, Malgieri G, Isernia C. β-Cyclodextrin inclusion complex to improve physicochemical properties of pipemidic acid: Characterization and bioactivity evaluation. International Journal of Molecular Sciences. 2013; Jun 25; 14(7): 13022-41. https://doi.org/10.3390/ijms140713022.
6. Erdő S M, Hartkamp R, Vlugt TJ, Moultos OA. Inclusion complexation of organic micropollutants with β-cyclodextrin. The Journal of Physical Chemistry B. 2020; Jan 24; 124(7): 1218-28. https://doi.org/10.1021/acs.jpcb.9b10122
7. Juzeniene A, Nizauskaite Z. Photodegradation of cobalamins in aqueous solutions and in human blood. Journal of Photochemistry and Photobiology B: Biology. 2013; May 5; 122: 7-14. https://doi.org/10.1016/j.jphotobiol.2013.03.001.
8. Kiran Q, Tania M, Zufi S, Iqbal A. Photodegradation of cyanocobalamin in the presence of ascorbic acid. emr-195283
9. Ahmad I, Hussain W, Fareedi AA. Photolysis of cyanocobalamin in aqueous solution. Journal of Pharmaceutical and Biomedical Analysis. 1992; Jan 1; 10(1): 9-15. https://doi.org/10.1016/0731-7085(92)80004-7.
10. Ahmad I, Ahmed S, Anwar Z, Sheraz MA, Sikorski M. Photostability and photostabilization of drugs and drug products. International Journal of Photoenergy. 2016; May 18; 2016. https://doi.org/10.1155/2016/8135608.
11. Giedyk M, Gryko D. Vitamin B12: An efficient cobalt catalyst for sustainable generation of radical species. Chem Catalysis. 2022; Jul 21; 2(7): 1534-48. https://doi.org/10.1016/j.checat.2022.05.004.
12. Lee W, Lee YB, Huh MH, Choi JK. Determination of the chemical stability of cyanocobalamin in medical food by a validated immunoaffinity column-linked HPLC method. Journal of Food Quality. 2022; Jan 12; 2022: 1-8. https://doi.org/10.1155/2022/1619936
13. Wang M, Schuster K, Asam S, Rychlik M. Challenges in the determination of total vitamin B12 by cyanidation conversion: insights from stable isotope dilution assays. Analytical and Bioanalytical Chemistry. 2023; Sep; 415(23): 5797-807. https://doi.org/10.1007/s00216-023-04860-y.
14. Rampazzo G, Zironi E, Pagliuca G, Gazzotti T. Analysis of Cobalamin (Vit B12) in Ripened Cheese by Ultra-High-Performance Liquid Chromatography Coupled with Mass Spectrometry. Foods. 2022; Sep 7; 11(18): 2745. https://doi.org/10.3390/foods11182745.
15. Devi S, Pasanna RM, Shamshuddin Z, Bhat K, Sivadas A, Mandal AK, Kurpad AV. Measuring vitamin B-12 bioavailability with [13C]- cyanocobalamin in humans. The American Journal of Clinical Nutrition. 2020; Dec; 112(6): 1504-15. https://doi.org/10.1093/ajcn/nqaa221.
16. Akbari N, Assadpour E, Kharazmi MS, Jafari SM. Encapsulation of Vitamin B12 by Complex Coacervation of Whey Protein Concentrate–Pectin; Optimization and Characterization. Molecules [Internet]. 2022; Sep 19; 27(18): 6130. https://doi.org/10.3390/molecules27186130
17. Lee W, Lee YB, Huh MH, Choi JK. Determination of the chemical stability of cyanocobalamin in medical food by a validated immunoaffinity column-linked HPLC method. Journal of Food Quality. 2022; Jan 12; 2022: 1-8. https://doi.org/10.1155/2022/1619936.
18. Ahmad I, Hussain W, Fareedi AA. Photolysis of cyanocobalamin in aqueous solution. Journal of Pharmaceutical and Biomedical Analysis. 1992; Jan 1; 10(1): 9-15. https://doi.org/10.1016/0731-7085(92)80004-7.
19. Ahmad I, Qadeer K, Zahid S, Sheraz MA, Ismail T, Hussain W, Ansari IA. Effect of ascorbic acid on the degradation of cyanocobalamin and hydroxocobalamin in aqueous solution: a kinetic study. AAPS PharmSciTech. 2014; Oct; 15: 1324 -33. https://doi.org/10.1208%2Fs12249-014-0160-5
20. Barman BK, Rajbanshi B, Yasmin A, Roy MN. Exploring inclusion complexes of ionic liquids with α-and β-cyclodextrin by NMR, IR, mass, density, viscosity, surface tension and conductance study. Journal of Molecular Structure. 2018 May 5; 1159:205 -15. https://doi.org/10.1016/j.molstruc.2018.01.062
21. Bodur S, Erarpat S, Balçık U, Bakırdere S. A rapid, sensitive and accurate determination of cobalamin with double monitoring system: HPLC-UV and HPLC-ICP-OES. Food Chemistry. 2021; Mar 15; 340: 127945. https://doi.org/10.1016/j.foodchem.2020.127945
22. Xu X, Peng S, Bao G, Zhang H, Yin C. β-cyclodextrin inclusion complexes with vitamin A and its esters: A comparative experimental and molecular modeling study. Journal of Molecular Structure. 2021; Jan 5; 1223: 129001. https://doi.org/10.1016/j.molstruc.2020.129001
23. Cid-Samamed A, Rakmai J, Mejuto JC, Simal-Gandara J, Astray G. Cyclodextrins inclusion complex: Preparation methods, analytical techniques and food industry applications. Food Chemistry. 2022; Aug 1; 384: 132467. https://doi.org/10.1016/j.foodchem.2022.132467.
24. Mazzocato MC, Thomazini M, Favaro-Trindade CS. Improving stability of vitamin B12 (Cyanocobalamin) using microencapsulation by spray chilling technique. Food Research International. 2019; Dec 1; 126: 108663. https://doi.org/10.1016/j.foodres.2019.108663.
25. Carlan IC, Estevinho BN, Rocha F. Study of microencapsulation and controlled release of modified chitosan microparticles containing vitamin B12. Powder Technology. 2017; Aug 1; 318: 162-9. https://doi.org/10.1016/j.powtec.2017.05.041.
26. Kumar SS, Chouhan RS, Thakur MS. Trends in analysis of vitamin B12. Analytical Biochemistry. 2010; Mar 15; 398(2): 139 -49. http://dx.doi.org/10.1016/j.ab.2009.06.041.
27. Estevinho BN, Carlan I, Blaga A, Rocha F. Soluble vitamins (vitamin B12 and vitamin C) microencapsulated with different biopolymers by a spray drying process. Powder Technology. 2016; Feb 1; 289: 71-8. https://doi.org/10.1016/j.powtec.2015.11.019
28. Amrutha N, Anand P, Swathy KR, Anila KN, Kumar SR. A study on the assessment of clinical profile and treatment pattern of multiple sclerosis in a tertiary care hospital. Research Journal of Pharmacy and Technology. 2020; 13(12): 6066-72. http://dx.doi.org/10.5958/0974-360X.2020.01057.4
29. Haddad NI, Abdulrahman NA. Correlation of Vitamin B12 deficiency with H. pylori infection and other Biochemical parameters in Iraqi patients. Research Journal of Pharmacy and Technology. 2020; Nov 13; 13(11): 5451-4. DOI: 10.5958/0974-360X.2020.00951.8
30. Vyas A, Gidwani B, Tripathi A, Dhruve P. Significance of jobs plot in cyclodextrin complexation. Research Journal of Pharmacy and Technology. 2016; 9(7): 1013-6. http://dx.doi.org/10.5958/0974-360X.2016.00191.8
31. Sarode SM, Mittal M, Sharma P, Shrivastava B, Gajare GK, Vidyasagar G. Preparation and Evaluation of Inclusion Complexes Using Cyclodextrins and Its Derivatives. Research Journal of Pharmacy and Technology. 2011;4(11):1761-5. Retrieved from https://ischolar.sscldl.in/index.php/Ajrc/article/view/140176
32. Rathi S, Patel D, Shah S. Physicochemical characterization and in-vitro dissolution behavior of artemether and lumefantrine: hydroxypropyl-β-cyclodextrin inclusion complex. Research Journal of Pharmacy and Technology. 2020;13(3):1137-41. http://dx.doi.org/10.5958/0974-360X.2020.00209.7
33. Rangasamy M, Balasubramaniam A, Gummadevelly S. Design and Evaluation of β-cyclodextrin Complexes of Meloxicam Tablet. Research Journal of Pharmacy and Technology. 2008; 1(4): 484-6.
34. Kulkarni AS, Dias RJ, Ghorpade VS, Mali KK. Freeze dried multicomponent inclusion complexes of piperine with cyclodextrin and hydrophilic polymers: Physicochemical characterization and In vivo anti-inflammatory activity. Research Journal of Pharmacy and Technology. 2020; 13(10): 4916-24. http://dx.doi.org/10.5958/0974-360X.2020.00864.1
35. Kulkarni NS, Ranpise NS, Dhole SN, Mohan G. Physico-Chemical Evaluation and In-vitro Release Studies of Irbesartan: β-Cyclodextrin: Soluplus Ternary Inclusion Complex. Research Journal of Pharmacy and Technology. 2014; 7(9): 987-94.
36. Kushwaha P. Significance of Stability Studies on Degradation Product. Research Journal of Pharmacy and Technology. 2009; 2(4): 621-7