Author(s):
Sidarningsih, Yuliati, Deny Saputra, Nastiti Faradila Ramadhani, Sasha Aprilia Rochmat, Bunga Fauzia, Mohammed Ahmed Aljunaid, Huda Rashad Qaid, Rini Devijanti Ridwan, Shuhdi Gamal Alaghbari, Mogeb Al-Nahari, Habib Thabet
Email(s):
rini-d-r@fkg.unair.ac.id
DOI:
10.52711/0974-360X.2025.00130 
Address:
Sidarningsih1, Yuliati1, Deny Saputra2,3, Nastiti Faradila Ramadhani3, Sasha Aprilia Rochmat4, Bunga Fauzia5,6, Mohammed Ahmed Aljunaid7,8, Huda Rashad Qaid2,8, Rini Devijanti Ridwan1*, Shuhdi Gamal Alaghbari7, Mogeb Al-Nahari 9, Habib Thabet 10,11
1Department of Oral Biology Faculty of Dental Medicine Universitas Airlangga, Surabaya.
2Doctoral Program of Dental Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
3Department of Dento-maxillofacial Radiology, Faculty of Dental Medicine Universitas Airlangga, Surabaya, Indonesia.
4Undergraduate Student, Faculty of Dental Medicine Universitas Airlangga, Surabaya, Indonesia.
5Magister of Dental Health, Faculty of Dental Medicine Universitas Airlangga Surabaya, Indonesia.
6Faculty of Dental Medicine Hang Tuah University, Surabaya, Indonesia.
7Department of Oral and Dental Medicine, Faculty of Medicine, Taiz University, Taiz, Yemen.
8Faculty of Oral and Dental Medicine, AL-Saeed University, Taiz
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Background/purpose: According to The Global Burden of Disease Study in 2016, oral health problems, especially periodontal disease, become the 11th most common globally, including dental cavities, periodontal disease, and oral fungal infection. Epigallocatechin-3-gallate (EGCG), the composition of green tea, has excellent benefits and plays a vital role in health, especially in regulating oral pathogenic bacteria activity that causes oral inflammation. Stem cells of human exfoliated deciduous teeth (SHED) metabolites also have great benefits and play an essential role in the health sector, especially as an alternative to regenerative therapy with its many bioactive activities. This study aims to prove the antimicrobial power of the SHED metabolite combined with EGCG against Fusobacterium nucleatum (F. nucleatum), Lactobacillus acidophilus (L. acidophilus), and Candida albicans (C. albicans). Materials and methods: The six different passages of SHED were prepared in Dulbecco’s Modified Eagle medium and added with EGCG. After 48 hours of incubation, the Mueller-Hinton agar medium diffusion method measured the inhibition zone. The research data was analyzed statistically. Results: The SHED metabolite was antibacterially active combined with EGCG against L. acidophilus and F. nucleatum bacteria, but there was no antifungal activity against the fungus C. albicans. Conclusion: The SHED metabolite combined with EGCG had antibacterial properties against L. acidophilus and F. nucleatum bacteria but did not have antifungal properties against C. albicans fungi.
Cite this article:
Sidarningsih, Yuliati, Deny Saputra, Nastiti Faradila Ramadhani, Sasha Aprilia Rochmat, Bunga Fauzia, Mohammed Ahmed Aljunaid, Huda Rashad Qaid, Rini Devijanti Ridwan, Shuhdi Gamal Alaghbari, Mogeb Al-Nahari, Habib Thabet. Antimicrobial effect of Stem cells from human exfoliated deciduous teeth metabolites combined with Epigallocatechin-3-gallate: As In vitro study. Research Journal of Pharmacy and Technology.2025;18(2):885-2. doi: 10.52711/0974-360X.2025.00130
Cite(Electronic):
Sidarningsih, Yuliati, Deny Saputra, Nastiti Faradila Ramadhani, Sasha Aprilia Rochmat, Bunga Fauzia, Mohammed Ahmed Aljunaid, Huda Rashad Qaid, Rini Devijanti Ridwan, Shuhdi Gamal Alaghbari, Mogeb Al-Nahari, Habib Thabet. Antimicrobial effect of Stem cells from human exfoliated deciduous teeth metabolites combined with Epigallocatechin-3-gallate: As In vitro study. Research Journal of Pharmacy and Technology.2025;18(2):885-2. doi: 10.52711/0974-360X.2025.00130 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-62
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