Author(s):
Dyah Aryantini, Puji Astuti, Nunung Yuniarti, Subagus Wahyuono
Email(s):
puji_astuti@ugm.ac.id
DOI:
10.52711/0974-360X.2025.00126 
Address:
Dyah Aryantini1,2, Puji Astuti3*, Nunung Yuniarti4, Subagus Wahyuono3
1Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Sleman, Yogyakarta, 55281, Indonesia.
2Institut Ilmu Kesehatan Bhakti Wiyata, Kediri, Jl. KH. Wachid Hasyim 65, Kediri, 64114, Indonesia.
3Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Sleman, Yogyakarta, 55281, Indonesia.
4Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Sleman, Yogyakarta, 55281, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Obesity prevention by targeting the inhibition of pancreatic lipase enzyme currently attracts some researchers, especially studies aiming to determine natural products' antiobesity activity. Their natural ingredients have a variety of chemical structures, which are expected to have potential as an antiobesity agent. Kaempferia rotunda is a plant that has the potential to inhibit the activity of the pancreatic lipase enzyme. This study aims to test the inhibition of the Porcine Pancreatic Lipase (PPL) enzyme by fractions and Crotepoxide isolated from Kaempferia rotunda Ethanolic Extract (KRE). Isolation was carried out using bioassay-guided isolation with an in vitro pancreatic lipase enzyme inhibition test used for the assay. P-Nitro Phenyl Butyrate (PNPB) functions as the synthetic substrate. KRE was separated by solid-liquid partition to obtain n-hexane (HF), ethyl acetate (EAF), ethanol (EF), and the insoluble residue fraction (RF). EAF was the most potent fraction (inhibition of 30.54±0.95%). Vacuum Liquid Chromatography further separated this fraction to produce four fractions (Ea1-Ea4), Ea3 had the highest inhibition (48.29±1.26%). Compound 1 (colorless needle) was isolated using the P-TLC method from fraction Ea.3, was identified as Crotepoxide. Crotepoxide was showing a percent inhibition of 42.80±0.49%, while Orlistat was 74.04±0.13%. Inhibition of the pancreatic lipase enzyme suggests that these compounds may exert antiobesity through another mechanism of antiobesity.
Cite this article:
Dyah Aryantini, Puji Astuti, Nunung Yuniarti, Subagus Wahyuono. Crotepoxide from Kaempferia rotunda L. Inhibit Pancreatic lipase In vitro. Research Journal of Pharmacy and Technology.2025;18(2):857-2. doi: 10.52711/0974-360X.2025.00126
Cite(Electronic):
Dyah Aryantini, Puji Astuti, Nunung Yuniarti, Subagus Wahyuono. Crotepoxide from Kaempferia rotunda L. Inhibit Pancreatic lipase In vitro. Research Journal of Pharmacy and Technology.2025;18(2):857-2. doi: 10.52711/0974-360X.2025.00126 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-58
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