Author(s):
Muchlisyam Bachri, Lisda Rimayani Nasution, Mutiara Balqis Ginting
Email(s):
muchlisyam@usu.ac.id
DOI:
10.52711/0974-360X.2025.00122 
Address:
Muchlisyam Bachri1, Lisda Rimayani Nasution1, Mutiara Balqis Ginting2
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Campus USU, Padang Bulan, Medan, 20155. Indonesia.
2Undergaraduate student, Faculty of Pharmacy, Universitas Sumatera Utara, Campus USU, Padang Bulan, Medan 20155, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
The study aims to extract and quantify the levels of quercetin and ascorbic acid in tomatoes simultaneously without separation. This is achieved through the creation of a spectrophotometric method using mean centering of ratio spectra.The preparation of the absorption spectrum for ascorbic acid, and quercetin with methanol, and measuring mean centering of ratio spectra of ascorbic acid at a wavelength at 238nm, regression equation Y = 0,0279X – 0,0004 and quercetin at 206 nm are Y = 0,1787X+0,1402. The levels of quercetin and ascorbic acid in tomatoes are obtained from quercetin at 470mg/100g Tomato fruit or percentage was (0.47±0.03)% and ascorbic acid at 1310mg/100g or (1.31±0.007)%. The validation parameters, namely exactness, correctness, continuance, LOD, and LOQ, indicate the mean centering of ratio spectra method for determination of simultaneous quercetin and ascorbic acid in tcmato fruit meets the requirements of the ICH guidelines.
Cite this article:
Muchlisyam Bachri, Lisda Rimayani Nasution, Mutiara Balqis Ginting. Extraction and determination of quercetin and ascorbic acid simultaneously in tomatoes (Solanum lycopersicum L.) using mean centering of ratio spectra. Research Journal of Pharmacy and Technology.2025;18(2):823-0. doi: 10.52711/0974-360X.2025.00122
Cite(Electronic):
Muchlisyam Bachri, Lisda Rimayani Nasution, Mutiara Balqis Ginting. Extraction and determination of quercetin and ascorbic acid simultaneously in tomatoes (Solanum lycopersicum L.) using mean centering of ratio spectra. Research Journal of Pharmacy and Technology.2025;18(2):823-0. doi: 10.52711/0974-360X.2025.00122 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-54
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