Author(s):
Siti Rahayu, Aulia Umi Rohmatika, Ufairanisa Islamatasya, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Djoko Santoso, Odi Yoshitaka Anggarda, Sri Puji Astuti Wahyuningsih, Win Darmanto
Email(s):
windarmanto@fst.unair.ac.id
DOI:
10.52711/0974-360X.2025.00119 
Address:
Siti Rahayu1, Aulia Umi Rohmatika2, Ufairanisa Islamatasya2, Raden Joko Kuncoroningrat Susilo3, Suhailah Hayaza3, Djoko Santoso4, Odi Yoshitaka Anggarda5, Sri Puji Astuti Wahyuningsih6, Win Darmanto6,7*
1Doctoral Program in Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
2Magister Program in Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
3Department of Nanotechnology Engineering, Faculty of Advance Technology and Multidiscipline, Universitas Airlangga, Surabaya, 60115, Indonesia.
4Faculty of Medicine, Universitas Airlangga, Surabaya, 60115, Indonesia.
5Rumah Sakit Umum Daerah Sayang, Cianjur, 43216, Indonesia.
6Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
7Institute of Science, Technology, and Health, Jombang 61419, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Carcinogenic Diethylnitrosamine (DEN) compounds are a class of nitrosamine compounds that have a negative impact on the body, often found in food preservatives. The increasing amount of DEN will damage the spleen organ so that the functional spleen organ decreases. The damage can include degeneration, intracellular accumulation, necrosis, and inflammation. Pro-inflammatory cytokines including TNF-a and IL-6 have an effect on tumor growth, IL-6 cytokines function as trophic factors for malignancy of cancer cells, TNF-a plays a role in cancer cell inflammation. Polysaccharides contained in G. applanatum such as ß-glucan have immunomodulating properties that can be developed as a strong biological response for cancer therapy. This study will examine the potential of G. applanatum polysaccharide extract on the percentage of apoptosis, necrosis, TNF-a and IL-6 levels in DEN-induced mice. Female DDY mice were randomly divided into 4 groups (n=6). The research method was carried out by comparing the normal control group (KN) given water; negative control (K-) given DEN 100mg/kg BW; positive control (K+) given DEN 100mg/kg BW and Doxorubicin 10 mg/kg BW; and treatment (P) given DEN 100mg/kg BW and G. applanatum extract 150mg/kg BW. G. applanatum polysaccharide extract showed a significant increase in the percentage of apoptosis and necrosis. G. applanatum polysaccharide extract 150mg/kg BW showed a significant decrease in TNF-a and IL-6 levels. In summary, the polysaccharides extract of G. applanatum are promising potential anticancer treatment.
Cite this article:
Siti Rahayu, Aulia Umi Rohmatika, Ufairanisa Islamatasya, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Djoko Santoso, Odi Yoshitaka Anggarda, Sri Puji Astuti Wahyuningsih, Win Darmanto. The Potential of Ganoderma applanatum Polysaccharides Extracts on Apoptosis, Necrosis, TNF-α, and IL-6 in Diethylnitrosamine-Induced Mice. Research Journal of Pharmacy and Technology.2025;18(2):809-4. doi: 10.52711/0974-360X.2025.00119
Cite(Electronic):
Siti Rahayu, Aulia Umi Rohmatika, Ufairanisa Islamatasya, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Djoko Santoso, Odi Yoshitaka Anggarda, Sri Puji Astuti Wahyuningsih, Win Darmanto. The Potential of Ganoderma applanatum Polysaccharides Extracts on Apoptosis, Necrosis, TNF-α, and IL-6 in Diethylnitrosamine-Induced Mice. Research Journal of Pharmacy and Technology.2025;18(2):809-4. doi: 10.52711/0974-360X.2025.00119 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-51
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