Author(s): Hanafi, Sotya Rawiningtyas, Henny Rochaeny, Sri Redjeki Setyawati, Alvina Nur Aini, Adya Rizky Pradipta

Email(s): sotyarawiningtyas@gmail.com

DOI: 10.52711/0974-360X.2025.00114   

Address: Hanafi1, Sotya Rawiningtyas1*, Henny Rochaeny2, Sri Redjeki Setyawati1, Alvina Nur Aini1, Adya Rizky Pradipta2
1Department of Food Industrial Quality Assurance, Polytechnic AKA Bogor, Bogor 16154, Indonesia.
2Department of Analytical Chemistry, Polytechnic AKA Bogor, Bogor 16154, Indonesia.
*Corresponding Author

Published In:   Volume - 18,      Issue - 2,     Year - 2025


ABSTRACT:
Betadine plant (Jatropha muktifidia Linn.) is traditionally used as a medicine that can heal wounds and pain due to accidents slashes. However, research on the total flavonoids and toxicity in plant was rarely done. To analyze the total flavonoids toxicity in the extract of betadine, betadine’s leaves and stems were extracted using methanol. The phytochemical assay revealed that all extracts contain Alkaloids, Flavonoids, Saponins, Sterol, and Phenol. The total flavonoids of Betadine leave extract were higher than Betadine stem extract. Betadine leaves extract has 10,36±0,03mg E Q/g and otherwise, Betadine stem extract has 1,13±0,02mg E Q/g of total flavonoids. A toxicity test was being to Artemia saline with BSLT (Brine Shrime Lethality Test) methods. The result showed that Betadine leaves and stem extract has high toxicity because their LC-50 was 29,29ppm and 27,79ppm.


Cite this article:
Hanafi, Sotya Rawiningtyas, Henny Rochaeny, Sri Redjeki Setyawati, Alvina Nur Aini, Adya Rizky Pradipta. Determination of Total Flavonoids and BSLT Methanol Extract of Leaves and STEM Betadine (Jatropha multifida Linn.). Research Journal of Pharmacy and Technology.2025;18(2):773-6. doi: 10.52711/0974-360X.2025.00114

Cite(Electronic):
Hanafi, Sotya Rawiningtyas, Henny Rochaeny, Sri Redjeki Setyawati, Alvina Nur Aini, Adya Rizky Pradipta. Determination of Total Flavonoids and BSLT Methanol Extract of Leaves and STEM Betadine (Jatropha multifida Linn.). Research Journal of Pharmacy and Technology.2025;18(2):773-6. doi: 10.52711/0974-360X.2025.00114   Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-46


REFERENCES:
1.    Apriyanti M. Tanaman Obat Paling Berkhasiat & Paling Dicari. Yogyakarta: Pustaka Baru Pres. 2010
2.    Shweta Singh Chauhan, Pramod Kumar Mahish. Flavonoids of the flame of forest- Butea monosperma. Research J. Pharm. and Tech. 2020. 13(11)
3.    Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand and Mohamed Gasim Abdullah. Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica Lin., Leaves. Research J. Pharm. and Tech. 2010. 3(2)
4.    Singh D, Sharma SK, Rani R, Mishra S and Sharma RA. A New Flavonoid and other Two Flavonoids Isolated from Different Plant Parts of Selected Cassia Species. Asian Journal of Research in Chemistry. 2011; 4(5): 818-821.
5.    Franyoto YD, Kusmita L, Mutmainah , Angrena RD. Total Flavonoid Content and Formulation Antioxidant Cream Stem of Jatropha multifida. IOP Conf. Series: Journal of Physics: Conf. 2018. 1025
6.    Nagaharika Y, Kalyani V, Rasheed S, Karthikeyan R, Anti-inflammatory activity of leaves of Jatropha gossypifolia L. by HRBC membrane stabilization method. Journal of Acute Disease. 2013. 2 (2): 156–158
7.    Shlini P and Siddalinga MKR. Extraction of Phenolics, Proteins and Antioxidant Activity from Defatted Tamarind Kernel Powder. Asian Journal of Research in Chemistry. 2011; 4(6); 936-941.
8.    Edeoga HO, Okwu DE, Mbaebre BO. Phytochemical Constituent of Some Nigerian Medicinal Plants. Afr Journal of Biotechnology. 2005; 4: 685 – 688
9.    Al-Owaisi, Mooza, Al-Hadiwi N, Khan SA. GC-MS analysis, determination of total phenolics, flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves. Asian Pac J. Trop Biomed. 2014; 4(12): 964-970
10.    Pisutthanan S, Plianbangchang P, Pisutthanan N, Ruanruay S, Muanrit O. Brine shrimp lethality activity of Thai medicinal plants in the family Meliaceae. Naresuan Uni J. 2004; 12: 13-18.
11.    Robards K, Prernzler PD, Tucker G, Swatsitang P, Glover W. Phenolic compounds and their role in oxidative processes in fruits. Food Chemistry. 1999; 66: 401-36
12.    Tekeshwar Kumar, Vishal Jain. Phytochemical Screening, Phenolic, Flavonoids, Carotenoids contents and antioxidant activity of Folkloric Memecylon edule roxb. Research J. Pharm. and Tech. 2016; 9(10)
13.    Chang CC, Yang MH and Wen HM. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10 (3); 178-182
14.    Tuapattinaya, Prelly MJ, Rumahlatu D. Analysis of Flavonoid Levels of Enhalus acoroides in Different Coastal Waters in Ambon Island, Indonesia. International Journal of Applied Biology. 2019; 3(1)
15.    Manurung H, Kustiawan W, Kusuma IW, Marjenah. Total flavonoid content and antioxidant activity of tabat Barito (Ficus deltoidea Jack) on different plant organs and ages. Journal of Medicinal Plants Studies. 2017; 5(6): 120-125
16.    Som K. Madhvi, Manik Sharma , Javaid Iqbal , Mohd Younis , Razda Sheikh. Phytochemical analysis, Total Flavonoid, Phenolic contents and Antioxidant activity of extracts from the Leaves of Rhododendron arboretum. Research J. Pharm. and Tech. 2020; April 13(4).
17.    Szliszka E et al. Chalcones Enhances TRAIL- Induced Apoptosis in prostate Cancer Cells. International Journal of Molecular Science. 2011; 11(1): 1-13
18.    Md. Saif Uddin, Mohammad Shah Hafez Kabir, and Syed Md. Abdul Kader. Brine shrimp lethality bioassay, thrombolytic and antibacterial activities of methanol extract of Boehmeria platyphylla d don leaves Int J Pharm 2016; 6(1): 29-36.
19.    Mamata, A. Brine Shrimp Lethality Test of Andrographis paniculate. Research J. Pharm. and Tech. 2014; 7(7).
20.    Ashpak. M. Tamboli, Kiran. A. Wadkar. Anticancer activity of leaves extracts of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) by Brine Shrimp Lethality Bioassay. Research J. Pharm. and Tech. 2021; 14(10).
21.    Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, Mc Laughlin JL. Brine Shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 1982; 45: 31-34.

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