Author(s):
Salima Amari, Ahlem Karbab, Noureddine Charef, Lekhmici Arrar
Email(s):
ahlem.karbab@univ-setif.dz , charefnr@univ-setif.dz
DOI:
10.52711/0974-360X.2025.00112 
Address:
Salima Amari1,2, Ahlem Karbab1*, Noureddine Charef1, Lekhmici Arrar1
1Laboratory of Applied Biochemistry, Faculty of Natural and Life Science, Ferhat Abbas University, Setif-1, 19000 Algeria.
2University Center of Abd Alhafid Boussouf, Mila, 43000, Algeria.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
The methanolic extract and different sub-fractions of the Erica arborea L. stems were used in the current study to determine their potential for a some range of activities ; the antioxidant activities were tested by 2,2- diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing assays, and its antimicrobial potential were evaluated against six pathogenic bacteria (Bacillus cereus, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Salmonella gallinarum, Staphylococcus aureus), three fungal strains (Aspergillus niger, Aspergillus flavus and Aspergillus brasiliensis and one yeast (Candida albicans). Minimum inhibitory concentration was determined using serial dilution microplates method. Comparative study of Methanolic, chloroform, ethyle acetate and aqueous extracts of E. arborea L. stems were realized for of its naturel polyphenols composition, antioxidant and antimicrobial properties. In addition, all these sub-fractions were used for preliminary quantification of certain important secondary bio-molecules. It was found the ethyl acetate extract of stems shows highest amount of polyphenols and flavonoids content. Whereas, the aqueous extract exhibited the highest value of condensed tannins. Stems ethyl acetate and methanolic extracts exhibited the highest antioxidant activity with the lowest IC50 values of 0.02mg/mL for DPPH and 0.006mg/mL for ferric reducing power. Among the various extracts, methanol extract showed moderate antibacterial activity against bacteria. P. aeruginosa bacteria strain is found to be more sensitive to the ethyl acetate stems extract of E. arborea (17.60±0.85mm) with MIC and MBC respectives values of 1.56±0.25 and 2.51±0.27mg/mL. The yeast and fungal strains have n’t presented any sensibility to all stems extracts tested. This study also showed the antioxidant potential of E. arborea. L stems and the great medicinal importance of its natural phytochemicals.
Cite this article:
Salima Amari, Ahlem Karbab, Noureddine Charef, Lekhmici Arrar. Antioxidant, Antimicrobial activities and Phytochemical analysis of Erica arborea L. stem extracts. Research Journal of Pharmacy and Technology.2025;18(2):757-4. doi: 10.52711/0974-360X.2025.00112
Cite(Electronic):
Salima Amari, Ahlem Karbab, Noureddine Charef, Lekhmici Arrar. Antioxidant, Antimicrobial activities and Phytochemical analysis of Erica arborea L. stem extracts. Research Journal of Pharmacy and Technology.2025;18(2):757-4. doi: 10.52711/0974-360X.2025.00112 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-44
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