Author(s):
Chung Dinh Duong, Chau Nguyen Thi Bao, Linh Dinh Thi Lan, Yen Thi Ngoc Nguyen
Email(s):
ddchung@ntt.edu.vn
DOI:
10.52711/0974-360X.2025.00110 
Address:
Chung Dinh Duong*, Chau Nguyen Thi Bao, Linh Dinh Thi Lan, Yen Thi Ngoc Nguyen
Faculty of Pharmacy, Nguyen Tat Thanh University, 298-300A Nguyen Tat Thanh, Ward 13, District 4, Ho Chi Minh City, 700000, Vietnam.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Leaf extracts of Mallotus barbatus Muell.-Arg., a plant species of the Euphorbiaceae family, were studied for the morphological and bioactive characteristics. The phylogenetic relationship was analyzed using DNA barcoding of the rbcl and ITS genes. Notably, ethyl acetate extract exhibits vigorous antibacterial, antifungal, antioxidant and cytotoxic activities, emphasizing its pharmaceutical potential. Furthermore, n-hexane and chloroform fractions showed promising inhibition of MDA-MB-231 breast cancer cell proliferation, with IC50 values of 42.81±0.72µg/ml and 52.91±0.93µg/ml, respectively. These findings comprehensively understand the genetic basis and therapeutic potential of Mallotus barbatus Muell.-Arg., providing valuable insights for medical and pharmaceutical applications.
Cite this article:
Chung Dinh Duong, Chau Nguyen Thi Bao, Linh Dinh Thi Lan, Yen Thi Ngoc Nguyen. Identification and Biological activity of leaf extracts from Mallotus barbatus Muell. - Arg. in Lam Dong province, Vietnam. Research Journal of Pharmacy and Technology.2025;18(2):744-0. doi: 10.52711/0974-360X.2025.00110
Cite(Electronic):
Chung Dinh Duong, Chau Nguyen Thi Bao, Linh Dinh Thi Lan, Yen Thi Ngoc Nguyen. Identification and Biological activity of leaf extracts from Mallotus barbatus Muell. - Arg. in Lam Dong province, Vietnam. Research Journal of Pharmacy and Technology.2025;18(2):744-0. doi: 10.52711/0974-360X.2025.00110 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-42
REFERENCES:
1. Bahadur S, Ahmad M, Long W, et al. Leaf epidermal traits of selected Euphorbiaceae and Phyllanthaceae taxa of Hainan island and their taxonomic relevance. Diversity. 2022; 14(10): 881.doi: 10.3390/d14100881
2. Sierra S, Van Welzen, and C P. A taxonomic revision of Mallotus section Mallotus (Euphorbiaceae) in Malesia. Blumea. 2005; 50(2): 249-74.doi: 10.3767/000651905X622987
3. Rivière C, Hong VNT, Pieters L, et al. Polyphenols isolated from antiradical extracts of Mallotus metcalfianus. Phytochemistry. 2009; 70(1): 86-94.doi: 10.1016/j.phytochem.2008.10.008
4. Mondal M, Hossain MM, Hasan MR, et al. Hepatoprotective and antioxidant capacity of Mallotus repandus ethyl acetate stem extract against d-galactosamine-induced hepatotoxicity in rats. ACS Omega. 2020; 5(12): 6523-31. doi: 10.1021/acsomega.9b04189
5. Chen J, Zhang X, Huo D, et al. Preliminary characterization, antioxidant and α-glucosidase inhibitory activities of polysaccharides from Mallotus furetianus. Carbohydrate Polymers. 2019; 215: 307-15.doi: 10.1016/j.carbpol.2019.03.099
6. Li S, Zhang W, Wang R, et al. Screening and identification of natural α-glucosidase and α-amylase inhibitors from partridge tea (Mallotus furetianus Muell-Arg) and in silico analysis. Food Chemistry. 2022; 388: 133004. doi: 10.1016/j.foodchem.2022.133004
7. Kiem PV, Nhiem NX, Anh NH, et al. Enantiomeric chromene derivatives with anticancer effects from Mallotus apelta. Bioorganic Chemistry. 2020; 104: 104268. doi: 10.1016/j.bioorg.2020.104268
8. Chattopadhyay D, Arunachalam G, Mandal AB, et al. Antimicrobial and anti-inflammatory activity of folklore: Mallotus peltatus leaf extract. Journal of Ethnopharmacology. 2002; 82(2): 229-37. doi: 10.1016/S0378-8741(02)00165-4
9. Zhang B, Lai L, Tan Y, et al. Hepatoprotective effect of total flavonoids of Mallotus apelta (Lour.) Muell.Arg. leaf against carbon tetrachloride-induced liver fibrosis in rats via modulation of TGF-β1/Smad and NF-κB signaling pathways. Journal of Ethnopharmacology. 2020; 254: 112714. doi: 10.1016/j.jep.2020.112714
10. Tistaert C, Chataigné G, Dejaegher B, et al. Multivariate data analysis to evaluate the fingerprint peaks responsible for the cytotoxic activity of Mallotus species. Journal of Chromatography B. 2012; 910: 103-13. doi: 10.1016/j.jchromb.2012.10.001
11. Anh NH, Park S, Trang DT, et al. Genus Mallotus (Euphorbiaceae): A review on traditional medicinal use, phytochemistry and biological activities. Vietnam Journal of Science and Technology. 2022; 60(2): 141-74. doi: 10.15625/2525-2518/16634
12. Rivière C, Nguyen Thi Hong V, Tran Hong Q, et al. Mallotus species from Vietnamese mountainous areas: phytochemistry and pharmacological activities. Phytochemistry Reviews. 2010; 9(2): 217-53. doi: 10.1007/s11101-009-9152-6
13. Van Hong NT, Rivière C, Hong QT, et al. Identification by LC-ESI-MS of flavonoids responsible for the antioxidant properties of Mallotus species from Vietnam. Natural Product Communications. 2011; 6(6): 813-8. doi
14. Van Welzen P, Middleton D, and Lindsay S. Flora of Singapore precursors, 21: New records of Euphorbiaceae for Singapore. Gardens' Bulletin Singapore. 2020; 72: 143-58. doi: 10.26492/gbs72(2).2020-03
15. Giang PM, Trang VM, and Son PT. Phytochemical studies on Mallotus barbatus. Chemistry of Natural Compounds. 2013; 49(1): 129-30. doi: 10.1007/s10600-013-0530-3
16. Rivière C, Thi Hong VN, Hoai NN, et al. N-methyl-5-carboxamide-2-pyridone from Mallotus barbatus: A chemosystematic marker of the Euphorbiaceae genus Mallotus. Biochemical Systematics and Ecology. 2012; 44: 212-5.doi: 10.1016/j.bse.2012.05.004
17. Salleh WMNHW, Ghani NA, and Khamis S. Chemical composition of Mallotus barbatus essential oil. Chemistry of Natural Compounds. 2023; 59(3): 576-7. doi: 10.1007/s10600-023-04058-9
18. Hussin KH, Wahab BA, and Teh CP. Comparative leaf anatomical studies of some Mallotus Lour. (Euphorbiaceae) species. Botanical Journal of the Linnean Society. 2008; 122(2): 137-53. doi: 10.1111/j.1095-8339.1996.tb02069.x
19. Plunkett GM, Soltis DE, and Soltis PS. Higher level relationships of Apiales (Apiaceae and Araliaceae) based on phylogenetic analysis of rbcL sequences. American Journal of Botany. 1996; 83(4): 499-515. doi: 10.2307/2446219
20. Kondo K, Terabayashi S, Okada M, et al. Phylogenetic relationship of medicinally important Cnidium officinale and Japanese Apiaceae based on rbcL sequences. Journal of Plant Research. 1996; 109(1): 21-7. doi: 10.1007/BF02344283
21. Poczai P, and Hyvönen J. Nuclear ribosomal spacer regions in plant phylogenetics: problems and prospects. Molecular Biology Reports. 2010; 37(4): 1897-912.doi: 10.1007/s11033-009-9630-3
22. Besse P. Book Nuclear ribosomal RNA genes: ITS Region. Springer US, New York, NY. 2021.
23. Gupta J. Preliminary phytochemical investigation, antioxidant and antimicrobial activity of Jasminum pubescence leaves extracts. Research Journal of Pharmacy and Technology. 2020; 13(12): 6073-6.doi: 0.5958/0974-360X.2020.01058.6
24. Thakur M, Sharma K, Mehta S, et al. Phytochemicals, antimicrobial and antioxidant potential of methanolic extract of Berberis aristata roots. Research Journal of Pharmacy and Technology. 2020; 13(12): 5763-7. doi: 10.5958/0974-360X.2020.01004.5
25. Awouafack MD, McGaw LJ, Gottfried S, et al. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC Complementary and Alternative Medicine. 2013; 13(1): 289. doi: 10.1186/1472-6882-13-289
26. Elshikh M, Ahmed S, Funston S, et al. Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology Letters. 2016; 38(6): 1015-9. doi: 10.1007/s10529-016-2079-2
27. Mostafa AA, Al-Askar AA, Almaary KS, et al. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences. 2018; 25(2): 361-6. doi: 10.1016/j.sjbs.2017.02.004
28. Moghaddam M, Miran SNK, Pirbalouti AG, et al. Variation in essential oil composition and antioxidant activity of cumin (Cuminum cyminum L.) fruits during stages of maturity. Industrial Crops and Products. 2015; 70: 163-9. doi: 10.1016/j.indcrop.2015.03.031
29. Fernandes V, D'lima A, Kumar MV, et al. In vitro antioxidant properties of Indian capsicum species-a comparative study. Research Journal of Pharmacy and Technology. 2019; 12(10): 4785-9.doi: 10.5958/0974-360X.2019.00826.6
30. Yadav AR, and Mohite SK. Antioxidant activity of Malvastrum coromandelianum leaf extracts. Research Journal of Topical and Cosmetic Sciences. 2020; 11(2): 59-61.doi: 10.5958/2321-5844.2020.00010.2
31. Yada D, Sivakkumar T, and Sudhakar M. Phytochemical evaluation and in-vitro antioxidant potential of whole plant of Caralluma adscendens. Research Journal of Pharmacy and Technology. 2021; 14(5): 2774-8. doi: 10.52711/0974-360X.2021.00489
32. Sharma GK, Sharma S, Chasta P, et al. Assessment of pharmacognostic parameters and antioxidant potential of bitter melon or karela (Momordica charantia L.) fruits by DPPH method. Research Journal of Pharmacy and Technology. 2021; 14(1): 437-41. doi: 10.5958/0974-360X.2021.00079.2
33. Xiao F, Xu T, Lu B, et al. Guidelines for antioxidant assays for food components. Food Frontiers. 2020; 1(1): 60-9. doi: 10.1002/fft2.10
34. Kulkarni K. Evaluation of anti-oxidant properties of some medicinal plant products by ABTS radical scavenging assay. Research Journal of Science and Technology. 2022; 14(4): 213-8. doi: 10.52711/2349-2988.2022.00035
35. Brintha S, Sivaraj C, Saraswathi K, et al. Antioxidant, antibacterial activities and GC-MS analysis of methanol extract of buds of Hypericum hookerianum Wight and Arnott. Research Journal of Pharmacy and Technology. 2020; 13(8): 3709-14. doi: 10.5958/0974-360X.2020.00656.3
36. Aravanan P, and Jayakumari S. In-vitro cytotoxic and antioxidant activity of Bauhinia racemosa root. Research Journal of Pharmacy and Technology. 2021; 14(6): 3333-6.doi: 10.52711/0974-360X.2021.00579
37. Joicy MC, Sivaraj C, and Arumugam P. In-vitro antioxidant, antidiabetic, antibacterial and cytotoxic activities of essential oil extracted from flowers of Illicium verum L. Research Journal of Pharmacy and Technology. 2021; 14(5): 2452-8. doi: 10.52711/0974-360X.2021.00431