Author(s):
Yohana A. Sinaga, Hadyanto Lim, Jekson Martiar Siahaan
Email(s):
jekson.siahaan.sked@gmail.com
DOI:
10.52711/0974-360X.2025.00109 
Address:
Yohana A. Sinaga1, Hadyanto Lim2, Jekson Martiar Siahaan3*
1Master's Student in Biomedical Science, Faculty of Medicine, Universitas Methodist Indonesia.
2Department of Pharmacology, Faculty of Medicine, Universitas Methodist Indonesia.
3Department of Physiology, Faculty of Medicine, Institut Kesehatan Deli Husada, Delitua, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Diabetes mellitus is a metabolic disorder characterized by hyperglycemia, oxidative stress, and inflammation, which can lead to various complications, including alterations in reproductive function. In this study, we aimed to investigate the effect of Coffea canephora var. Robusta ethanol extract on testosterone and cysteine-rich secretory protein-1 (CRISP-1) levels in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were divided into five groups: control, diabetic, and diabetic rats treated with Coffea canephora var. Robusta ethanol extract at three different doses (100, 200, and 400mg/kg body weight). After four weeks of treatment, serum testosterone and CRISP-1 levels were measured. The results showed that STZ-induced diabetes significantly decreased testosterone levels and increased CRISP-1 expression compared to the control group. However, treatment with Coffea canephora var. Robusta ethanol extract at all three doses significantly increased testosterone levels and decreased CRISP-1 expression in diabetic rats. Furthermore, the extract at the highest dose (400mg/kg) exhibited the most significant effect. These findings suggest that Coffea canephora var. Robusta ethanol extract may have therapeutic potential in regulating testosterone and CRISP-1 levels in STZ-induced diabetic rats, which could have implications for the management of diabetes-associated reproductive complications. Further studies are needed to elucidate the underlying mechanisms and evaluate the long-term effects of Coffea canephora var. Robusta ethanol extract on diabetes and its complications.
Cite this article:
Yohana A. Sinaga, Hadyanto Lim, Jekson Martiar Siahaan. Coffea canephora var. Robusta Modulates Testosterone and CRISP-1 Levels in STZ-Induced Diabetic Rats. Research Journal of Pharmacy and Technology.2025;18(2):739-3. doi: 10.52711/0974-360X.2025.00109
Cite(Electronic):
Yohana A. Sinaga, Hadyanto Lim, Jekson Martiar Siahaan. Coffea canephora var. Robusta Modulates Testosterone and CRISP-1 Levels in STZ-Induced Diabetic Rats. Research Journal of Pharmacy and Technology.2025;18(2):739-3. doi: 10.52711/0974-360X.2025.00109 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-41
REFERENCES:
1. Situmorang PC, Ilyas S, Syahputra RA, Nugraha AP, Putri MSS, Rumahorbo CGP. Rhodomyrtus tomentosa (Aiton) Hassk. (haramonting) protects against allethrin-exposed pulmo damage in rats: mechanistic interleukins. Front Pharmacol. 2024; 15: 1343936. Published 2024 Feb 6. doi:10.3389/fphar.2024.1343936
2. Sharma P. K., Kumar P. A. Antidiabetic Potential of Ocimum Basilicum Nanoparticles in Diabetic Rats. J. Diabetes Res. 2022; 2022: 5603748. doi.org/10.1155/2022/5603748
3. Nurkolis F, Kurniawan R, Wiyarta E, et al. Unraveling Light-Activated Insulin Action in Regulating Blood Glucose: New Photoactivatable Insight as a Novel Modality in Diabetes Management. Molecules. 2024;29(6):1294.
4. Prajoko YW, Qhabibi FR, Gerardo TS, et al. Revealing Novel Source of Breast Cancer Inhibitors from Seagrass Enhalus acoroides: In Silico and In vitro Studies. Molecules. 2024; 29(5): 1082. Published 2024 Feb 29. doi:10.3390/molecules29051082
5. Prananda AT, Dalimunthe A, Harahap U, et al. Phyllanthus emblica: a comprehensive review of its phytochemical composition and pharmacological properties. Front Pharmacol. 2023; 14: 1288618.
6. Widyawati T, Syahputra RA, Syarifah S, Sumantri IB. Analysis of Antidiabetic Activity of Squalene via In Silico and In vivo Assay. Molecules. 2023; 28(9): 3783. Published 2023; Apr 27. doi:10.3390/molecules28093783
7. Syahputra RA, Harahap U, Harahap Y, et al. Vernonia amygdalina Ethanol Extract Protects against Doxorubicin-Induced Cardiotoxicity via TGFβ, Cytochrome c, and Apoptosis. Molecules. 2023; 28(11): 4305. Published 2023 May 24. doi:10.3390/molecules28114305
8. Haendler, B., Kratzschmar, J., Theuring, F., and Schleuning, W. D. Transcripts for cysteine-rich secretory protein-1 (Crisp-1; de/aeg) and the novel related crisp-3 are expressed under androgen control in the mouse salivary gland. Endocrinology. 1993: 133(1): 192-198. https://doi.org/10.1210/endo.133.1.8319566
9. Jalkanen, J., Huhtaniemi, I., and Poutanen, M. Mouse cysteine-rich secretory protein 4 (CRISP4): A member of the Crisp family exclusively expressed in the epididymis in an androgen-dependent manner. Biology of Reproduction. 2005; 72(5): 1268-1274. https://doi.org/10.1095/biolreprod.104.035758
10. Krätzschmar, J., Haendler, B., Eberspaecher, U., Roosterman, D., Donner, P., and Schleuning, W. D. The human cysteine-rich secretory protein (CRISP) family: Primary structure and tissue distribution of CRISP-1, CRISP-2, and CRISP-3. European Journal of Biochemistry. 1996; 236(3): 827-836. https://doi.org/10.1111/j.1432-1033.1996.t01-1-00827.x
11. Mizuki, N., Sarapata, D. E., Garcia-Sanz, J. A., and Kasahara, M. The mouse male germ cell-specific gene Tpx-1: Molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes. Mammalian Genome. 1992; 3(5): 274-280. https://doi.org/10.1007/BF00292155
12. Rochwerger, L., Cohen, D. J., and Cuasnicú, P. S. (1992). Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion. Developmental Biology. 1992; 153(1): 83-90. https://doi.org/10.1016/0012-1606(92)90093-V
13. Rochwerger, L., and Cuasnicu, P. S. Redistribution of a rat sperm epididymal glycoprotein after in vitro and in vivo capacitation. Molecular Reproduction and Development. 1992; 31(1): 34-41. https://doi.org/10.1002/mrd.1080310107
14. Udby, L., Bjartell, A., Malm, J., Egesten, A., Lundwall, Å., Cowland, J. B., Borregaard, N., and Kjeldsen, L. Characterization and localization of Cysteine-Rich Secretory Protein 3 (CRISP-3) in the human male reproductive tract. Journal of Andrology. 2005; 26(3): 333-342. https://doi.org/10.2164/jandrol.04132
15. Busso, D., Cohen, D. J., Maldera, J. A., Dematteis, A., and Cuasnicu, P. S. A novel function for CRISP1 in rodent fertilization: Involvement in sperm-zona pellucida interaction. Biology of Reproduction. 2007; 77(5): 848-854. https://doi.org/10.1095/biolreprod.107.061788
16. Cohen, D. J., Da Ros, V. G., Busso, D., Ellerman, D. A., Maldera, J. A., Goldweic, N., and Cuasnicú, P. S. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation. Asian Journal of Andrology. 2007; 9(4): 528-532.
17. Haendler, B., Kratzschmar, J., Theuring, F., and Schleuning, W. D. Transcripts for cysteine-rich secretory protein-1 (Crisp-1; de/aeg) and the novel related crisp-3 are expressed under androgen control in the mouse salivary gland. Endocrinology. 1993; 133(1): 192-198. https://doi.org/10.1210/endo.133.1.8319566
18. Jalkanen, J., Huhtaniemi, I., and Poutanen, M. Mouse cysteine-rich secretory protein 4 (CRISP4): A member of the Crisp family exclusively expressed in the epididymis in an androgen-dependent manner. Biology of Reproduction. 2005; 72(5): 1268-1274. https://doi.org/10.1095/biolreprod.104.035758